30,535 results

1. QUAS-5-T7-GFP_ColE1

   Plasmid

   #171653

   Purpose

   QUAS five base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   QUAS-5-T7
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. QUAS-10-T7-GFP_ColE1

   Plasmid

   #171654

   Purpose

   QUAS ten base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   QUAS-10-T7
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. QUAS-15-T7-GFP_ColE1

   Plasmid

   #171655

   Purpose

   QUAS fifteen base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   QUAS-15-T7
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK642

   Plasmid

   #72391

   Purpose

   Produces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)

   Depositor

   T. J. Kappock

   Insert

   glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Mutated tyrosine-397 to phenylalanine to block ad…

   Promoter

   T7

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKD3_mCherry

   Plasmid

   #187385

   Purpose

   To chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRT

   Depositor

   Roi Avraham

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Human PNP his-tagged

   Plasmid

   #64076

   Purpose

   Expression of human Purine Nucleoside Phosphorylase

   Depositor

   Vern Schramm

   Article

   Lewandowicz et al J Biol Chem. 2003 Aug 22;278(34):31465-8

   Insert

   Purine Nucleoside Phosphorylase (PNP Human)
   

   Use

   Tags

   Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pAWP78-PVCpnf_pvc13-antiMouseMHCIINb

   Plasmid

   #198292

   Purpose

   Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc13-antiMouseMHCIINb
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET29b-AK1-His

   Plasmid

   #124135

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Adenylate kinase (AK1 Chicken)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD24-sfGFPx2

   Plasmid

   #51559

   Purpose

   Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)

   Depositor

   Francisco Malagon, Sankar Adhya

   Article

   Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

   Insert

   superfolder GFP
   

   Use

   Tags

   GLESTCRHASLAVLADERRFSA and MARARA

   Expression

   Bacterial

   Mutation

   Contains additonal aa in the N and C terminus (in…

   Promoter

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. ICMAB - pET22b T7 EncFtn Mms6

    Plasmid

    #225538

    Purpose

    Construction of intracellular magnetite accumulating bacterial systems.

    Depositor

    Urartu Seker

    Article

    Yavuz et al Small. 2022 Jul;18(26):e2200537. doi: 10

    Inserts

    Iron binding protein
    Ferroxidase
    Material binding peptite
    Iron transporter protein
    

    Use

    Tags

    no

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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