33,449 results
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Plasmid#126600Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only
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"AND-gate" receiver plasmid
Plasmid#127676PurposeEncodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.DepositorInsertLuxR, GFP
UseSynthetic BiologyExpressionBacterialPromoterpLlacO-1 for LuxR, pLux for GFPAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP26-1A(sfGFP)
Plasmid#126973PurposeLevel 1 vector. sfGFP reporter. Origin p15A (medium copy number)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT29-splitD
Plasmid#122599Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
ExpressionBacterialAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBT100.106b
Plasmid#122601Purposeclone APOBEC1 phage by SapI Golden GateDepositorInsertAPOBEC1
ExpressionBacterialAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
Reb_206 aka PAS629
Plasmid#71234PurposeExpresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherryDepositorPublicationInsertReb locus
TagsmNeonGreen to second copy RebB, soluble mCherry a…ExpressionBacterialPromoterT7Available sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP45-2A(sfGFP)
Plasmid#126989PurposeLevel 2 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP25-1A(sfGFP)
Plasmid#126972PurposeLevel 1 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET_MBP-RGG-RGG
Plasmid#124946PurposeExpression of MBP-RGG-RGGDepositorAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
nifF in pET21b
Plasmid#124520PurposeAzotobacter vinelandii flavodoxin II of NitrogenaseDepositorAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
p15aC-GFP
Plasmid#107862PurposeControl plasmid expressing GFPDepositorInsertGFP
TagsmycExpressionBacterialPromoterproDAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTn7xTS-sfGFP
Plasmid#117390PurposeTn7 tagging vector pTn7xTS with sfGFP expression scaffoldDepositorInsertsfGFP
UseSynthetic BiologyExpressionBacterialPromoterPtacAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p1-GST-ArcΔCTD
Plasmid#119878PurposeBacterial Expression construct for the production and purification of Arc missing C-terminal DomainDepositorInsertActivity Regulated Cytoskeletal Protein (Arc Rat)
TagsGSTExpressionBacterialMutationDeletion of nt 832-1110PromotertacAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12i2
Plasmid#120883PurposeExpresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.DepositorInsertCas12i2
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMM627
Plasmid#112532PurposepZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanRDepositorInsertsHrtR
ChuA
luxCDABE
ExpressionBacterialPromoterJ23107, PL(HrtO), and ProDAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-Myc
Plasmid#119757PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Myc tag.DepositorTypeEmpty backboneTagsGST and MycExpressionBacterialPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOGG011
Plasmid#113985PurposeVector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectorsDepositorInsertpBBR1
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOGG010
Plasmid#113984PurposeVector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectorsDepositorInsertRK2 (IncP)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJMP1354
Plasmid#119275Purposefor cloning new sgRNAsDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTn7xTS-dTomato
Plasmid#117391PurposeTn7 tagging vector pTn7xTS with dTomato expression scaffoldDepositorInsertdTomato
UseSynthetic BiologyExpressionBacterialPromoterPtacAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by