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pWT021a

Plasmid

#107895

Purpose

W2.0 plasmid. Contains PTetO-SD8-BE2, PLac-sgRNA1 and is part of CAMERA 2.0

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PLac-sgRNA1

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT004a

Plasmid

#107883

Purpose

R1 plasmid. Contains PBAD-EGFP and is part of the CAMERA system for rewritable multi-event analog recording in bacteria

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PBAD-EGFP

Expression

Bacterial

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a- mYFP-kanMX6

Plasmid

#105147

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mYFP-kanMX6

Tags

mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-mMaple3-kanMX6

Plasmid

#105165

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-4

Insert

mMaple3-kanMX6

Tags

GlySerGly-mMaple3

Expression

Bacterial and Yeast

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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GST-AR-1-566

Plasmid

#104195

Purpose

Expresses human AR-1-566 from bacterial expression vector pGEX-5X-1 as GST fusion

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

human androgen receptor aa 1-566 (AR Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2-Katushka

Plasmid

#104008

Purpose

Plasmid encoding Katushka

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

Katushka

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (E3V, K6T, N21D, R3…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mCherry2-L

Plasmid

#104027

Purpose

Plasmid encoding 2ndVal mCherry2-L

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mCherry2-L

Use

Low copy

Expression

Bacterial

Mutation

mCherry2-L with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-coSCFP3A

Plasmid

#104015

Purpose

Plasmid encoding coSCFP3A

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

coSCFP3A

Use

Low copy

Expression

Bacterial

Mutation

Same protein sequence as SCFP3A (codon optimized …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-T-Sapphire

Plasmid

#103977

Purpose

Plasmid encoding T-Sapphire

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

T-Sapphire

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (Q69M, C70V, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTO_CHMP2B_LAP

Plasmid

#101848

Purpose

Tet-inducible expression of human CHMP2B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

Depositor

Daniel Gerlich

Article

Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

Insert

CHMP2B (CHMP2B Human)

Use

Flp-frt recombination, tet-inducible expression

Tags

Localization and Affinity Purification (LAP) tag

Expression

Bacterial and Mammalian

Mutation

silent mutation in Thr 104; A to T

Promoter

CMV

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGST-DHFR Mm

Plasmid

#83073

Purpose

Expresses the full length DHFR Mm in E.coli

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

2x DHFR (Dhfr Mouse)

Tags

GST

Expression

Bacterial

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pL2020

Plasmid

#86709

Purpose

araBAD based expression vector for inducible protein production in bacterial hosts

Depositor

Hartmut Michel

Article

Sommer et al Microb Cell Fact. 2017 Sep 20;16(1):157.

Type

Empty backbone

Tags

His10 tag

Expression

Bacterial

Promoter

araBAD

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pDB070.iodo.5

Plasmid

#99397

Purpose

Expression plasmid encoding the evolved MjTyrRS variant Iodo.5RS for amber suppression with p-iodophenylalanine

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

PN25 TetR; Ptet Iodo.5RS; PproK tyrT(Opt,CUA)

Expression

Bacterial

Promoter

PN25, Ptet, and PproK

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pJPK13

Plasmid

#80775

Purpose

Cloning vector compatible with ColE1 plasmids with high copy number

Depositor

Gregory Phillips

Article

Peterson et al Plasmid. 2008 May;59(3):193-201. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pSG25-pBAD-tetO-deGFPssrA

Plasmid

#102452

Purpose

Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

deGFP

Tags

ssrA

Expression

Bacterial

Promoter

pBAD-tetO

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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5VBD (USP09X)

Plasmid

#101260

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

USP09X (USP9X Human)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pmVirD2-GFP

Plasmid

#90315

Purpose

mVirD2-GFP expression plasmid

Depositor

Bjorn Hogberg

Article

Bernardinelli et al Nucleic Acids Res. 2017 Oct 13;45(18):e1

Insert

A. tumefaciens VirD2 from aa 1 to 204 - GFP

Tags

6xHis and GFP

Expression

Bacterial

Promoter

T7/T5 IPTG inducible

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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PEP119T

Plasmid

#98251

Purpose

Pseudomonas T3SS gateway vector with HA-sfGFP11-sfGFP11

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

Gateway cassettee-HA-sfGFP11-11

Expression

Bacterial

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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HMBP-3C-STAR.66-285

Plasmid

#100094

Purpose

expresses MBP-tagged STARD1 protein domain (residues 66-285)

Depositor

Nikolai Sluchanko

Article

Sluchanko et al Protein Expr Purif. 2016 Mar;119:27-35.

Insert

STAR protein (STAR Human)

Tags

His-tagged MBP

Expression

Bacterial

Promoter

Ptac

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pTKEI-tLOV

Plasmid

#100574

Purpose

Expression plasmid for the thermostable anaerobic fluorescent protein tLOV

Depositor

David Savage

Article

Higgins et al ACS Synth Biol. 2017 Jul 24. doi: 10.102

Insert

tLOV

Tags

6xHis

Expression

Bacterial

Promoter

pTrc

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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