31,797 results
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Plasmid#98514PurposeBB1 with insert: promoter PAOX1DepositorInsertAOX1 promoter
ExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARL8B
Plasmid#67383PurposeExpression of native hArl8b in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAD-PSmOrange
Plasmid#31886DepositorInsertPSmOrange
ExpressionBacterialMutationS21T, Q63L, F100Y, L125M, K166R, P192S (mutations…Available SinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDG04163
Plasmid#216373PurposexhNup214-Nb1t (+2Cys) Nanobody productionDepositorInsertxhNup214-Nb1t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-scSUMOExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
peSCBE3-NG-Hypa
Plasmid#218165PurposeThis plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in StreptomycesDepositorInsertsescbe3-NG-Hypa
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionExpressionBacterialMutationMutation in the portion of deaminase hAPOBEC3A : …PromoterrpsL promoterAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pProEx N-Ras(G12C) CL
Plasmid#224262PurposeProtein purification of N-Ras (G12C)DepositorAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pProEx H-Ras(G12C) CL
Plasmid#224261PurposeProtein purification of H-Ras (G12C)DepositorAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET14b – lambda exonuclease
Plasmid#104531Purposebacterial expression of lambda exonucleaseDepositorInsertλ exonuclease
TagsHis6 followed by a thrombin siteExpressionBacterialPromoterT7Available SinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDA303
Plasmid#195199PurposeOverexpressing 6His-TetR in E. coli.DepositorInsertTetR
Tags6xHisExpressionBacterialPromoterT5 promoterAvailable SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET14-GFP30_Encap
Plasmid#86406PurposeExpresses sfGFP tagged with a 30 amino acid CLP and encapsulin as an operonDepositorInsertssfGFP with a 30aa CLP tag
Encapsulin
Tags6x HistidineExpressionBacterialPromoterT7Available SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
LB1554 (Bcl-2 promoter with MHS1234, Cdx mut)
Plasmid#15384DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterial and MammalianMutationBcl-2 full promoter with IgH full enhancer MHS123…Available SinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
p40phox-PX (1-144)
Plasmid#119123PurposeBacterial expression of human phox homology (PX) domain, p40phox-PX (1-144)DepositorInsertp40phox-PX (1-144)
TagsGSTExpressionBacterialPromotertacAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPPC010.AAV
Plasmid#171175PurposeExpression of Sp.pCas9-dCas9, BBa_J23107-MCP-SoxS(R93A/S101A), and hAAVS1 scRNA on pBBR1-KmR plasmidDepositorInsertshAAVS1 scRNA
Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterBBa_J23119 and Sp.pCas9, BBa_J23107Available SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAX1
Plasmid#117397Purposeallelic exchange vector with GFP merodiploid tracker and temperature-sensitive origin of replicationDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Omega 1
Plasmid#118046PurposeGoldenBraid2.0 Omega 1 destination vectorDepositorTypeEmpty backboneUseSynthetic Biology; Assembly of gb2.0 transcriptio…ExpressionBacterial, Insect, and Mamm…Available SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGT-B3
Plasmid#122581PurposeMAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)DepositorInsertsfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-Cas12a-recX
Plasmid#158714PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. marinum and recXDepositorInsertrecX
UseCRISPRExpressionBacterialPromoterPmycAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28C-SARS-CoV-2-Nucleocapsid
Plasmid#204402PurposeE. coli expression of full-length SARS-CoV-2 nucleocapsidDepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
KLHL12
Plasmid#38908PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -