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pRL542

Plasmid

#70282

Purpose

ColK-based vector; provides a nicking function to permit conjugal transfer and bears a polylinker for insertion of methylase-encoding genes

Depositor

Peter Wolk

Article

Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pDM_T7_HisLacZomega

Plasmid

#118815

Purpose

T7 RNAP-driven expression of N-terminal His-tagged lacZ omega subunit.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

lacZ_

Tags

histag

Expression

Bacterial

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pOPINB-HOIL-1 full-length (1–510)

Plasmid

#193858

Purpose

Expression of human His-tagged HOIL-1 (RBCK1) full-length (1–510) codon optimized for E. coli

Depositor

Bernhard Lechtenberg

Article

Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:

Insert

HOIL-1 (RBCK1 Human)

Tags

3C protease cleavage site and 6x-His tag

Expression

Bacterial

Mutation

Codon optimised for expression in E. coli

Promoter

T7

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPBAD-yhjH

Plasmid

#111613

Purpose

Arabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjH

Depositor

Tim Tolker-Nielsen

Article

Christensen et al Infect Immun. 2013 Aug;81(8):2705-13. do

Insert

yhjH

Expression

Bacterial

Mutation

A52V

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-Cdk9 T186A

Plasmid

#28101

Depositor

Andrew Rice

Article

Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

Insert

Cdk9 (CDK9 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Contains a T186A mutation in Cdk9

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2-HA-GAAZFP-FokI-RR

Plasmid

#18754

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

zinc finger nuclease

Expression

Bacterial

Mutation

RNA expression vector for expressing the zinc fin…

Available Since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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pLG021 (AVAST type 5)

Plasmid

#157899

Purpose

Expresses the AVAST type 5 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

AVAST type 5 (STAND ATPase with N-terminal SIR2)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pdCas9-omega

Plasmid

#114632

Purpose

Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

Depositor

Anushree Chatterjee

Article

Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

Insert

dCas9-omega

Use

CRISPR

Expression

Bacterial

Promoter

Tet promoter

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV WT SPOP 28-359

Plasmid

#194115

Purpose

Wild type His-SUMO SPOP expression plasmid

Depositor

Tanja Mittag

Article

Thomasen et al Elife. 2023 Mar 1;12:e84147. doi: 10.755

Insert

SPOP (SPOP Human)

Tags

6His-SUMO-TEV

Expression

Bacterial

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2E1

Plasmid

#15787

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2E1 (UBE2E1 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pGEX SH3(2)-3R

Plasmid

#112091

Purpose

Bacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-3R (NCK1 Human)

Tags

GST

Expression

Bacterial

Promoter

Lac

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-iRFP720

Plasmid

#45460

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Expression

Bacterial

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

araBAD

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pSF-CjPglB-LpxE

Plasmid

#128389

Purpose

Expression of C. jejuni PglB and F. tularensis LpxE in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Inserts

PglB oligosaccharylktransferase
LpxE phosphatase

Tags

Flag tag

Expression

Bacterial

Promoter

Ptac and pBAD

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBB528

Plasmid

#27390

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

lacIq

Expression

Bacterial

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046785

Plasmid

#145684

Purpose

Bacterial Expression plasmid for SARS-CoV-2 N (nucleocapsid)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;StrepII

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-IMX-DogTag

Plasmid

#113765

Purpose

Expresses IMX-DogTag in bacterial cells for modular antigen multimerization using SnoopLigase

Depositor

Mark Howarth

Article

Andersson et al Sci Rep. 2019 Mar 15;9(1):4625. doi: 10.

Insert

IMX-DogTag

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSBY3_ligD-HygR

Plasmid

#104625

Purpose

CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.

Depositor

Sheng Yang

Article

Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

Insert

FnCpf1

Use

CRISPR

Expression

Bacterial

Mutation

FnCpf1 codon-optimized C.glutamicum

Promoter

hsp60

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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mKO2-pBAD

Plasmid

#54555

Purpose

Localization: Protein Expression Vector , Excitation: 551, Emission: 565

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Sakaue-Sawano et al Cell. 2008 Feb 8;132(3):487-98. doi: 10.

Type

Empty backbone

Tags

6xHis and mKO2

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196, G175R)-Strep

Plasmid

#196211

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

G175R

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196, G189E)-Strep

Plasmid

#196212

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

G189E

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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