30,535 results

1. peSCBE3-NG-Hypa

   Plasmid

   #218165

   Purpose

   This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   escbe3-NG-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutation in the portion of deaminase hAPOBEC3A : …

   Promoter

   rpsL promoter

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. IGF2BP1_2xRRM-4xKH_pGEX

   Plasmid

   #135122

   Purpose

   Recombinant expression of IGF2BP1 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   IGF2BP1 (IGF2BP1 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBSV2G_PflaB-msfGFPBb

   Plasmid

   #118231

   Purpose

   Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfGFP

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Insert

   flagellin promoter driven msfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. lacI-PT5-gusA-pBAV1k

   Plasmid

   #30501

   Depositor

   Ichiro Matsumura

   Article

   Murin et al Appl Environ Microbiol. 2011 Oct 21.

   Insert

   beta-glucuronidase (gusA Escherichia coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMAL-p5X

   Plasmid

   #150814

   Purpose

   Expresses proteins in the periplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

   Depositor

   Paul Riggs

   Type

   Empty backbone

   Use

   Tags

   malE A313V

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   July 22, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pProEx N-Ras(G12C) CL

   Plasmid

   #224262

   Purpose

   Protein purification of N-Ras (G12C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   N-Ras (NRAS Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G12C

   Promoter

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pProEx H-Ras(G12C) CL

   Plasmid

   #224261

   Purpose

   Protein purification of H-Ras (G12C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   H-Ras (HRAS Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G12C

   Promoter

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDiGi

   Plasmid

   #59323

   Purpose

   Inducible expression of dsRed and GFP in bacteria

   Depositor

   David Holden, Sophie Helaine

   Article

   Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

   Inserts

   EGFP
   dsRed
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pbad and Plac

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSCW01

   Plasmid

   #72300

   Purpose

   Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

   Depositor

   Peggy Hsieh

   Article

   Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

   Type

   Empty backbone

   Use

   Derivative of puc19

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAKlux2.1

    Plasmid

    #14081

    Depositor

    Attila Karsi

    Article

    Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

    Insert

    Bacterial luciferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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