30,807 results

1. C-BLa 1.2

   Plasmid

   #89000

   Purpose

   BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.

   Depositor

   Dieter Langosch

   Article

   Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

   Insert

   C-BLa 1.2 fusion protein
   

   Tags

   Flag

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. AF1521-c003

   Plasmid

   #173111

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
   

   Tags

   Nanoluc

   Expression

   Bacterial

   Mutation

   KH-M1-L192

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEM-Cas9HF1-indRecA56

   Plasmid

   #102294

   Purpose

   Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Inserts

   Cas9HF1
   recA56
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   pTet

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. His6_MBP_FXR2P_P474S_P493N

   Plasmid

   #198695

   Purpose

   Expression of human FXR2 protein in E. coli

   Depositor

   Simpson Joseph

   Article

   Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

   Insert

   FXR2P (FXR2 Human)
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Changed proline 474 to serine and proline 493 asp…

   Promoter

   T7

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AF1521-c002

   Plasmid

   #173109

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   KH-M1-L192

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-parkin

   Plasmid

   #227367

   Purpose

   For bacterial expression of parkin

   Depositor

   Kalle Gehring

   Article

   Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

   Insert

   Parkin (PRKN Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression.

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZE-PalkB-GFP

   Plasmid

   #44381

   Depositor

   Hal Alper

   Article

   Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. d

   Inserts

   GFP
   PalkB
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PalkB

   Available Since

   April 17, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. ReNL_pRSETb

   Plasmid

   #89536

   Purpose

   Red color variant of bright luminescent protein for bacterial expression

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   ReNL
   

   Tags

   His X 6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJV0047

   Plasmid

   #205139

   Purpose

   Expression of 6xHis-EGFP-2xPX in bacteria

   Depositor

   Jason King

   Article

   Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

   Insert

   tandem 2xPX domain of SnxA
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pColdII-SIRPT1-Eptesicus fuscus

    Plasmid

    #78113

    Purpose

    Nt His-tag Eptesicus fuscus Sacsin 89-1368

    Depositor

    Kalle Gehring

    Insert

    Sacsin Eptesicus fuscus 89-1368
    

    Tags

    His-Tag

    Expression

    Bacterial

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)

    Plasmid

    #169191

    Purpose

    To express nsp10-nsp16 fusion protein in E. coli

    Depositor

    John Diffley

    Article

    Basu et al Biochem J. 2021 Jul 16;478(13):2481-2497

    Insert

    14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab Synthetic, SARS-CoV-2)
    

    Tags

    14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJKR-H-cdaR

    Plasmid

    #62557

    Purpose

    Glucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Inserts

    beta lactamase
    sfGFP
    CdaR
    

    Expression

    Bacterial

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZa-tetO-csgB-CmR

    Plasmid

    #89580

    Purpose

    Express csgB curli protein

    Depositor

    Urartu Seker

    Article

    Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

    Insert

    csgB
    

    Expression

    Bacterial

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. MT1G

    Plasmid

    #105704

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 1g

    Depositor

    Artur Krężel

    Insert

    Metallothionein 1g (MT1G Human)
    

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJBL711

    Plasmid

    #140381

    Purpose

    Expresses OtrR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-OtrR-T
    

    Tags

    TEV-6XHis

    Expression

    Bacterial

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDL52 (clone45)

    Plasmid

    #188578

    Purpose

    High-yield production of coenzyme F420 in E. coli

    Depositor

    Gerald Lackner

    Article

    Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

    Inserts

    ribA
    ribD
    yigB
    fbiC
    cofC
    cofD
    cofI
    cofE
    

    Expression

    Bacterial

    Mutation

    Codon optimization, synthetic ribosome binding si…

    Promoter

    T7 variants

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET11a-Z-NGFP

    Plasmid

    #52734

    Purpose

    IPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assay

    Depositor

    Lynne Regan

    Article

    Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

    Insert

    NGFP+Z-fusion
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pA5c-tesA-vhb50-8fadR

    Plasmid

    #86601

    Purpose

    expression of tesA and Vhb50 under the control of a LacUV5 promoter and fadR under the control of a pBAD promoter

    Depositor

    Yinjie Tang

    Article

    Liu et al Biotechnol Bioeng. 2016 Aug 6. doi: 10.1

    Inserts

    tesA
    Vhb50
    fadR
    

    Expression

    Bacterial

    Promoter

    LacUV5 and pBAD

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. HSPA9A

    Plasmid

    #42469

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    HSPA9A (HSPA9 Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pSLC-246

    Plasmid

    #73190

    Purpose

    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

    Depositor

    Swaine Chen

    Article

    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

    Insert

    PrhaB-tse2
    

    Expression

    Bacterial

    Promoter

    PrhaB

    Available Since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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