31,059 results
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Plasmid#184556PurposeKnockdown of murine KRAS gene by targeting the promoter region via CRISPRi systemDepositorInsertKirsten rat sarcoma virus (Kras Mouse)
UseCRISPR, Lentiviral, and Mouse TargetingTagsFlagExpressionBacterialAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 2A protease; 6xHis-MBP-TEV tag, self cleaving
Plasmid#204811PurposeBacterial expresison of Enterovirus A71 2A proteaseDepositorInsertA71 2A protease
ExpressionBacterialAvailable SinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
BPK2014-Cas12i-Max
Plasmid#188276Purposeprokaryotic expressionDepositorInsertCas12i-Max
UseCRISPRExpressionBacterialMutationnonePromoterT7Available SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3187 HPV-16 E2
Plasmid#10846DepositorAvailable SinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
TS245_pNKI-2-13_EGFP-DPN7
Plasmid#200719Purposeproducing Methyl Tracer EGFP protein in bacteriaDepositorInserteGFP-DPN7
Tags6xHisExpressionBacterialAvailable SinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAND
Plasmid#49377PurposeExpression of AND gate in bacteriaDepositorInsertAND gate
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Biomolecular FeedBack (FB)
Plasmid#109391PurposeThis construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.DepositorInsertpJ23100mut-dCas9-T- phtpG1-sgRNA
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACACA
Plasmid#39083PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDD132
Plasmid#119885PurposeIntegration vector into the ROCI-3 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance markerDepositorInsertBbaJ23104_EYFP
ExpressionBacterialAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pHH0103_TP53BP2-1/1
Plasmid#91503PurposeProtein expression and purification of human SH3 domain construct TP53BP2-1/1DepositorInsertTP53BP2-1/1 (TP53BP2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKD233.7-3
Plasmid#90955PurposeExpresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutivelyDepositorInsertsTetrathionate response regulator
Superfolder GFP
mCherry
ExpressionBacterialPromoterBba_J23114, PltetO-1, and PttrB185-269Available SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHBFD1Bad
Plasmid#53564PurposeProduces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinoseDepositorInserthbfd1 cDNA
ExpressionBacterialPromoteraraBADAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-pro-IL18
Plasmid#183389PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Citroff1
Plasmid#134301PurposeBacterial expression of inverse-response citrate sensor with high binding affinityDepositorInsertCitroff1
Tags6-His tagExpressionBacterialPromoterpBADAvailable SinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4A-MMP8 Wt
Plasmid#29545DepositorAvailable SinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJCX3-ACTG2_R257C
Plasmid#228911PurposeExpresses mutant R257C smooth muscle actin gamma-2 in human cellsDepositorInsertActin gamma 2, smooth muscle (ACTG2 Human)
TagsHIS-FLAG-TEVExpressionBacterial and MammalianMutationR257CPromoterCMVAvailable SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJCX3-ACTG2_WT
Plasmid#228910PurposeExpresses wildtype smooth muscle actin gamma-2 in human cellsDepositorInsertActin gamma 2, smooth muscle (ACTG2 Human)
TagsHIS-FLAG-TEVExpressionBacterial and MammalianMutationWTPromoterCMVAvailable SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
PHB1
Plasmid#212153PurposeFor PHB production in E. coli (PT7-pMB1).DepositorInsertpoly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
ExpressionBacterialPromoterPT7Available SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC1430
Plasmid#127981PurposeAMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only