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Showing: 6021 - 6040 of 29477 results

  4. p12x601

    Plasmid
    #157785
    Purpose
    EcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backbone
    Depositor
    Michael Rosen
    Article
    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi
    Insert
    pMD2.G(spei/spei)&12x601_tetO
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    157785-plasmid-map-sequence-id-357805

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  8. pAM5082

    Plasmid
    #85121
    Purpose
    PkaiB-kaiC-ecfp expressed from NSII-Gm vector constructed by seamless assembly. Fusion expressed as full length fusion protein at WT levels but is 5hr long period. Used only for co-localization exp
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    PkaiB-KaiC-ECFP
    Use
    Tags
    ECFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85121-plasmid-map-sequence-id-180106

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  12. pET15b/GgVcl 884-1066-ECFP

    Plasmid
    #46182
    Depositor
    Susan Craig
    Insert
    Vcl 884-1066 (VCL Chicken)
    Use
    Tags
    ECFP and His
    Expression
    Bacterial
    Mutation
    aa 884-1066 (tail domain)
    Promoter
    T7
    Available since
    July 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. cryS96

    Plasmid
    #67519
    Purpose
    αB-crystallin minipeptide fused to ELP (S96).
    Depositor
    Andrew MacKay
    Article
    Wang et al J Control Release. 2014 Oct 10;191:4-14.
    Insert
    mini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Human, Synthetic)
    Use
    Tags
    elastin-like polypeptide (ELP) S96
    Expression
    Bacterial
    Mutation
    mini-αB-crystallin contains residues 73–92 of aB-…
    Promoter
    T7
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. pHis17_ParM _2

    Plasmid
    #78202
    Purpose
    Expression of double-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor
    Depositor
    Martin Webb
    Article
    Kunzelmann et al ACS Chem Biol. 2010 Apr 16;5(4):415-25.
    Insert
    parM (K33A D63C T174A T175N D224C C287A)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    K33A D63C T174A T175N D224C C287A
    Promoter
    unknown
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    78202-plasmid-map-sequence-id-146031

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  17. pDB038b

    Plasmid
    #99212
    Purpose
    Accessory plasmid for PACE of PylRS variants
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII(P29*,P83*,Y184*), luxAB; PproK pylT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codons at positions 29, 83, and 184 of gIII
    Promoter
    Ppsp and PproK
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99212-plasmid-map-sequence-id-192366

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Showing: 6021 - 6040 of 29477 results