30,304 results
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Plasmid#34626DepositorInsertMyoglobin
UseTags6xHISExpressionBacterialMutationcodon-optimizedPromoterlppAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJUMP45-2A(sfGFP)
Plasmid#126989PurposeLevel 2 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET11a+CYP119
Plasmid#66131PurposeExpresses the wild type of CYP119DepositorInsertCYP119 (cyp119 )
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET22-Nsp10-TEV-6His
Plasmid#154957PurposeExpresses Nsp10 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coliDepositorInsertNsp10-TEV-6His (ORF1ab SARS-CoV-2)
UseTagsTEV-6HisExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-4T HHARI RBR
Plasmid#31254DepositorInsertHuman Homologue of Ariadne (HHARI) (ARIH1 Human)
UseTagsGSTExpressionBacterialMutationRBR domain residues 177-395PromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-tev-mCherry
Plasmid#117752PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP, tev cleavage site and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, tev site, mCherry, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQCasTns(Ptr)-IS186
Plasmid#175581PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(IS186)
UseTagsExpressionBacterialMutationNOPromoterAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b
Plasmid#122850PurposeExpresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coliDepositorInsertm(KS_MAT)_mouseL_ACP (Fasn Mouse)
UseTagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-VtrA-mCherry
Plasmid#192862PurposepMUT2 based vector for sensing human secondary bile acids in E. coli Nissle 1917DepositorInsertVtrA-CadC fusion protein and cognate promoter inducible by secondary bile acids
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFGM1
Plasmid#64949Purposesource of GmR FRT cassetteDepositorInsertFRT-GmR-FRT
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKB231
Plasmid#170018PurposeEncodes LacI-controlled expression of surface-displayed cecropin P1 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Cecropin P1
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-143
Plasmid#118527PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6-TEV-mCherry
Plasmid#132430PurposeExpresses His6-tagged mCherry in E. coli using T7 promoterDepositorInsertmCherry
UseTagsN-terminal His6 tag with TEV protease siteExpressionBacterialMutationCodon optimizedPromoterT7Available sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBBR5pemIKpKan
Plasmid#129271PurposeBroad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and constitutive promoterDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
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(SIM)5
Plasmid#126947PurposeBacterial expression plasmid for (SIM)5 client to assess SUMO-SIM scaffolds.DepositorInsert5 repeats of SUMO interacting motif (SIM) from PIASx, each separated by (GGS)4 (PIAS2 Human)
UseTagsExpressionBacterialMutationPromoterunknownAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
P14-pZE-SUMO-ObiH
Plasmid#204629PurposeColE1 ori, KanR, TetR, Tet promoter with a codon optimized obiH gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.DepositorInsertHis-SUMO-TEV-ObiH
UseSynthetic BiologyTagsHis Tag; SUMO Tag; TEV Protease SiteExpressionBacterialMutationPromoterPLtetO-1 promoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
SYKA-c020
Plasmid#175489PurposeProtein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.DepositorInsertSYK (SYK Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
GDAP2-c005
Plasmid#173110PurposeExpression of recombinant fusion protein in E. coliDepositorInsertGDAP2 (GDAP2 Human)
UseTagsmCeruleanExpressionBacterialMutationM1-N231PromoterAvailable sinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB224
Plasmid#170014PurposeEncodes LacI-controlled expression of surface-displayed NC and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
NC
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only