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32,826 results

Showing: 9121 - 9150 of 32826 results

  2. pGEX6P1-human LIC1 C-terminal half

    Plasmid
    #74599
    Purpose
    used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523
    Depositor
    Ron Vale
    Article
    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554
    Insert
    human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
    Tags
    N-terminal GST and Strep II
    Expression
    Bacterial
    Mutation
    amino acids 389-523 of LIC1
    Promoter
    T7
    Available Since
    May 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    74599-plasmid-map-sequence-id-145961

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  6. MAGEA3

    Plasmid
    #73246
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAGEA3 (MAGEA3 Human)
    Tags
    His6-Thioredoxin-TEV
    Expression
    Bacterial
    Mutation
    pfam01454:MAGE 116-286
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73246-plasmid-map-sequence-id-137200

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  7. TP53BP1

    Plasmid
    #73252
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TP53BP1A (TP53BP1 Human)
    Tags
    His6-Thrombin
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73252-plasmid-map-sequence-id-136217

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  8. MAPK8

    Plasmid
    #73236
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAPK8 (MAPK8 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00069:Pkinase 26-321
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73236-plasmid-map-sequence-id-137188

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  10. pMD19T-psba1-Ppsba2-dCas9-SpR

    Plasmid
    #73220
    Purpose
    Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.
    Depositor
    Paul Hudson
    Article
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    dCas9 from S. pyogenes
    Tags
    c-myc
    Expression
    Bacterial
    Mutation
    Silent mutation at bp 1341 A->C to remove an E…
    Promoter
    PpsbA2
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73220-plasmid-map-sequence-id-134711

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  11. pCMK

    Plasmid
    #62818
    Purpose
    Bacterial constitutive S. pyogenes Cas9 expression plasmid
    Depositor
    Sébastien Rodrigue
    Insert
    Cas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available Since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    62818-plasmid-map-sequence-id-114423

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  13. pETM6-4F2-mCherry

    Plasmid
    #73421
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 4F2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P4F2 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73421-plasmid-map-sequence-id-135321

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  17. pGEX2TK-Skm1-PH

    Plasmid
    #71371
    Purpose
    bacterial expression of PH domain for phosphoinositide binding assay
    Depositor
    Mark Lemmon
    Article
    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.
    Insert
    Skm1-PH
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Pleckstrin homology (PH) domain; aa1-132
    Promoter
    tac
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. pJK266

    Plasmid
    #73326
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    violacein biosynthesis protein VioD
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73326-plasmid-map-sequence-id-135034

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  22. pJK647

    Plasmid
    #71701
    Purpose
    Produces E. coli thioredoxin reductase (EcTrxB)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin reductase
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71701-plasmid-map-sequence-id-131729

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  23. pDCAF3-IS5

    Plasmid
    #65220
    Purpose
    Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.
    Depositor
    Jeffrey Barrick
    Article
    Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.
    Inserts
    ndmA
    ndmB
    ndmC
    ndmD
    gst9
    chlR
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Changed start codon from gtg to atg and Changed s…
    Promoter
    BBa_J23100
    Available Since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    65220-plasmid-map-sequence-id-134450

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  27. pGEM4Z-OMA1

    Plasmid
    #61718
    Purpose
    human Oma1 protease in the E.coli/in vitro translation vector
    Depositor
    Oleh Khalimonchuk
    Insert
    OMA1 (OMA1 Human)
    Expression
    Bacterial
    Available Since
    Dec. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 9121 - 9150 of 32826 results