30,808 results

1. pBAD-MBP-His-ACPs

   Plasmid

   #141140

   Purpose

   Expression of holo-[acyl-carrier-protein] synthase

   Depositor

   New England Biolabs

   Insert

   acpS
   

   Tags

   MBP

   Expression

   Bacterial

   Available Since

   May 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pSU315

   Plasmid

   #169697

   Purpose

   Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.

   Depositor

   Nara Figueroa

   Article

   Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

   Insert

   1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
   

   Tags

   1xHA tag

   Expression

   Bacterial

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAN-PTet-dCas9

   Plasmid

   #62244

   Purpose

   controls the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   catalytically inactive, D10A, H840A

   Promoter

   Ptet

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET32a-DEST

   Plasmid

   #84652

   Purpose

   E. coli expression vector derived from pET32 with Gateway cassette.

   Depositor

   Neil Olszewski

   Type

   Empty backbone

   Tags

   His, S tag, and Trx Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH0103_GRB2-1/2

   Plasmid

   #91480

   Purpose

   Protein expression and purification of human SH3 domain construct GRB2-1/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   GRB2-1/2 (GRB2 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSETa-mMaple3-V157I

   Plasmid

   #98577

   Purpose

   photoconvertible fluorescent protein mMaple3-V157I in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

   Depositor

   Ulrike Endesfelder

   Article

   Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

   Insert

   mMaple3-V157I
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBXCA3H

   Plasmid

   #47072

   Purpose

   FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease site and 10x His tag

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Tags

   3C protease site (PreScission site), Avi-tag, and…

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSA1

   Plasmid

   #52261

   Purpose

   Bacterial expression of human 6His-tagged SUMO1 and SUMO-E1

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Inserts

   SUMO-1 (SUMO1 Human)
   SAE-2 (UBA2 Human)
   SAE-1 (SAE1 Human)
   

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   C-terminal gly-gly

   Promoter

   T7 and rbs

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAD12

   Plasmid

   #34832

   Depositor

   Cynthia Kenyon

   Article

   Dillin et al Science. 2002 Oct 25;298(5594):830-4.

   Insert

   None
   

   Use

   RNAi

   Expression

   Bacterial

   Available Since

   June 11, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pGEX-5X1 Ash2L full

    Plasmid

    #15549

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    Ash2L (ASH2L Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pMUT2-pTcpP-mCherry

    Plasmid

    #192861

    Purpose

    pMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    TcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids
    

    Expression

    Bacterial

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CD Ugi1

    Plasmid

    #177194

    Purpose

    Greengate entry vector pGGC000 modified to contain bladderwort insulator 1

    Depositor

    Wayne Parrott

    Article

    Park et al bioRxiv 2021.11.04.467280

    Insert

    Utricularia gibba insulator 1
    

    Expression

    Bacterial

    Available Since

    June 28, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCAT207

    Plasmid

    #134884

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. proISG15 (2-165)

    Plasmid

    #110762

    Purpose

    Bacterial expression of proISG15

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    ISG15 (ISG15 Human)
    

    Tags

    N-His6-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHH0103_ACTG2_Actin_2

    Plasmid

    #109990

    Purpose

    Protein expression and purification of ACTG2_Actin_2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    ACTG2_Actin_2 (ACTG2 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJS647

    Plasmid

    #183176

    Purpose

    PYR1 mutagenesis plasmid compatible with pJS646 Y2H vector

    Depositor

    Timothy Whitehead

    Article

    Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

    Insert

    PYR1 (PYR1 Mustard Weed)
    

    Expression

    Bacterial

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2T-CAG-SpCas9-BlastR

    Plasmid

    #107190

    Purpose

    Confers constitutive expression of SpCas9

    Depositor

    Richard Sherwood

    Article

    Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

    Insert

    SpCas9 (NEWENTRY )
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Bacterial and Mammalian

    Promoter

    Chicken ß-actin

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLJSRSF7

    Plasmid

    #64693

    Purpose

    His-MBP-Smt3-NpuDnaEN102-CBD

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Type

    Empty backbone

    Tags

    CBD, HisTag, MBP, NpuDnaE-N102, and Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSP2292

    Plasmid

    #70707

    Purpose

    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    E782K, N968K and R1015H in SaCas9

    Promoter

    T7

    Available Since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. p4027 GST-ubiquitin

    Plasmid

    #10861

    Depositor

    Peter Howley

    Article

    Scheffner et al Cell. 1993 Nov 5. 75(3):495-505.

    Insert

    ubiquitin (UBI4 Budding Yeast)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only