31,059 results
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Plasmid#149474PurposeBroad-Host range expression vector, pBBR origin, RhaR-RhaS activator, PrhaBAD promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertRhaS-RhaR-GFP
UseSynthetic BiologyExpressionBacterialPromoterPrhaBADAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET32a-DEST
Plasmid#84652PurposeE. coli expression vector derived from pET32 with Gateway cassette.DepositorTypeEmpty backboneTagsHis, S tag, and Trx TagExpressionBacterialPromoterT7Available SinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAB078d5
Plasmid#79204PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ppro1 434cI
ExpressionBacterialPromoterPlacZ-opt (OR1); Ppro1Available SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-mphR
Plasmid#62559PurposeMacrolide biosensor. High copy plasmid that expresses GFP proportional to macrolide concentration.DepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-araC
Plasmid#62563PurposeArabinose biosensor. High copy plasmid that expresses GFP proportional to arabinose concentration.DepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p1-GST-ArcΔCTD
Plasmid#119878PurposeBacterial Expression construct for the production and purification of Arc missing C-terminal DomainDepositorInsertActivity Regulated Cytoskeletal Protein (Arc Rat)
TagsGSTExpressionBacterialMutationDeletion of nt 832-1110PromotertacAvailable SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
N-BLa 1.2
Plasmid#88999PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.DepositorInsertN-BLa 1.2 fusion protein
TagsFlagExpressionBacterialPromoteraraBADAvailable SinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
C-BLa 1.2
Plasmid#89000PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.DepositorInsertC-BLa 1.2 fusion protein
TagsFlagExpressionBacterialPromoteraraBADAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
BPV00500 pET21GG2 His(6)-TF-BECN(1-450)
Plasmid#136684PurposeExpresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor)DepositorInsertBeclin-1 (BECN1 Human)
TagsTrigger FactorExpressionBacterialMutationDNA optimized for E. coli expressionPromoterT7Available SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mYPet
Plasmid#103988PurposePlasmid encoding mYPetDepositorInsertmYPet
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F46L, I47L, …PromoterproCAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X1 Flag WDR5
Plasmid#15553DepositorAvailable SinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin
Plasmid#227367PurposeFor bacterial expression of parkinDepositorInsertParkin (PRKN Human)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression.Available SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Gm-LacZ10
Plasmid#65026PurposeGmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 1. See Depositor Comments below for information regarding the lacZ sequence.DepositorTypeEmpty backboneTagslacZExpressionBacterialAvailable SinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC- AVITAG-CTH10STII- -GG
Plasmid#215805PurposeCloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tagDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-6xHis-mCherry(-6)
Plasmid#205045PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertmCherry(-6)
TagsMGHHHHHGGExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQH
Plasmid#48096PurposeCumate-inducible expression of HA-tagged fusion proteinDepositorTypeEmpty backboneTags3xFLAG and HAExpressionBacterialPromoterPQ5Available SinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGP-FF
Plasmid#13480DepositorTypeEmpty backboneTagsGal11PExpressionBacterialAvailable SinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKS17
Plasmid#24628DepositorInsertpdc, adhB, atfA
UseSynthetic BiologyExpressionBacterialAvailable SinceAug. 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
6BY9 (EHMT1)
Plasmid#119956PurposeBacterial expression for structure determinationDepositorAvailable SinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMM627
Plasmid#112532PurposepZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanRDepositorInsertsHrtR
ChuA
luxCDABE
ExpressionBacterialPromoterJ23107, PL(HrtO), and ProDAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only