30,304 results
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Plasmid#136943PurposeMalachite green aptamer, T7 promoterDepositorInsertmalachite green aptamer (MGA)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pATmin-GG
Plasmid#191352PurposeClostridium expression vector (pAMB1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pPP076
Plasmid#158794PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purificationDepositorInsertCasPhi-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAH121
Plasmid#164901PurposeThe pAH121 plasmid [Haldimann and Wanner 2001] is the phi21 helper plasmid necessary for genomic integration of the pCYMR expression plasmid.DepositorInsertphi21 Integrase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-pro-sumo1
Plasmid#25101DepositorInsertpro-SUMO1 (SUMO1 Human)
UseTagsHisExpressionBacterialMutationChanged Serine 2 to AlaninePromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pDonorGFP(Ptr)
Plasmid#175578PurposeDNA donor for transpositionDepositorInsertMini-Tn, GFP cassette
UseTagsExpressionBacterialMutationNOPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTW358f
Plasmid#227935PurposeExpress hIAPP-T7nDepositorInserthIAPP-T7n
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBL066c
Plasmid#227937PurposeExpress Aβ42-GFPDepositorInsertAβ42
UseTagsGFP (F64L, S65T)ExpressionBacterialMutationF64L and S65T in GFPPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB_syb25
Plasmid#197992PurposeExpressed sybody 25 as a C-terminal myc-His-tagged constructDepositorInsertSybody 25
UseTagsMyc-HisExpressionBacterialMutationPromoterArabinoseAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAP973-1
Plasmid#99496PurposeTEV::linker::meGFP:3XFlagDepositorInsertTEV::linker::meGFP::3XFlag
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCPP5233
Plasmid#128715PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcM-hopM1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
TS_pLuxLac
Plasmid#140426PurposeToggle switch circuit responsive to AHL gradient.DepositorInsertsGFP
LacI
TetR
mCherry
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSL0828 (pQCascade_crRNA-4)
Plasmid#130637PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5910
Plasmid#128717PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcO-hopO1-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5058
Plasmid#128710PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcF-hopF2
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM11-ACS
Plasmid#108943Purposeheterologous expression of ACS of E. coli in E. coliDepositorInsertAcetyl-CoA synthetase (ACS)
UseTagsHis6-TagExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
ASB9A-c009
Plasmid#53670Purposebacterial expression plasmidDepositorInsertASB9A
UseTagsHis6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEAT8-137 M1V
Plasmid#173807PurposeExpression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cellsDepositorInsertSERPiNA1 (SERPINA1 Human)
UseTagsExpressionBacterialMutationE1M Otherwise this plasmid encodes the most commo…PromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only