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pET28a-Ec.coaE (pESC124)

Plasmid

#50390

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

Depositor

Tadhg Begley, Erick Strauss

Article

Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

Insert

DPCK

Tags

6XHIS tag

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pGal4x5-E4T-G210

Plasmid

#171077

Purpose

The resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permission

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

Gal4-E4-Gless-cassette

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET-X(4130)B1

Plasmid

#168018

Purpose

Expresses X(4130)B1-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

X(4130)B1-6xHis

Tags

Hisx6

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pQexp

Plasmid

#25820

Depositor

George Church

Article

Tolonen et al Mol Microbiol. 2009 Dec . 74(6):1300-13.

Insert

pQexp

Expression

Bacterial

Available Since

Sept. 14, 2010

Availability

Academic Institutions and Nonprofits only

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MAPK8

Plasmid

#73236

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

MAPK8 (MAPK8 Human)

Tags

His-6-TEV

Expression

Bacterial

Mutation

pfam00069:Pkinase 26-321

Promoter

T7

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

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REM1

Plasmid

#55681

Purpose

expression of mouse REM1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

REM1 (Rem1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pPath-Violacein-pT7native

Plasmid

#200002

Purpose

Violacein pathway. pT7-native CDS sequence

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Expression

Bacterial

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pYTK004

Plasmid

#65111

Purpose

Encodes ConL2 as a Type 1 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

ConL2

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pBSSK WT p85 alpha flag

Plasmid

#13429

Depositor

Ronald Kahn

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (PIK3R1 Human)

Tags

flag

Expression

Bacterial

Available Since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pET-PhoCl1-6His

Plasmid

#164033

Purpose

Expressed PhoCl1 in a pET expression vector

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

PhoCl1

Tags

6xHis Tag

Expression

Bacterial

Promoter

T7 promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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bfloGFPa1

Plasmid

#60749

Purpose

Bacterial expression of Amphioxus GFPa1

Depositor

Dimitri Deheyn

Article

Bomati et al Sci Rep. 2014 Jun 27;4:5469. doi: 10.103

Insert

GFPa1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pWN172

Plasmid

#215291

Purpose

Expresses sfGFP under the Anderson J23113 promoter with an RSF1010 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Tags

His

Expression

Bacterial

Promoter

J23113

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBS FGF3 isoform 1

Plasmid

#19230

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Fibroblast growth factor receptor 3 isoform 1 (FGFR3 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
R711-X04-566: His6-MBP-tev-Hs.NF1iso2 (1198-1530)

Plasmid

#159579

Purpose

E. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)

Depositor

Dominic Esposito

Article

Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

Insert

His6-MBP-tev-Hs.NF1 DEF (1198-1530) (NF1 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pJCSUP35

Plasmid

#1088

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Tags

HIS tag

Expression

Bacterial

Available Since

May 26, 2005

Availability

Academic Institutions and Nonprofits only
pBbS8k-RFP_N99D

Plasmid

#109410

Purpose

RepA Mutant

Depositor

Jay Keasling

Article

Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

Insert

repA

Tags

RFP

Expression

Bacterial

Mutation

N99D repA mutation to increase copy number

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEB1-wtGFP

Plasmid

#103978

Purpose

Plasmid encoding wtGFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

wtGFP

Use

Low copy

Expression

Bacterial

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pBA1015

Plasmid

#189589

Purpose

T7gp1.7 deletion HR vector 250bp HR Arms (T7 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

T7gp1.7 Locus 250bp Homology Arms

Use

Synthetic Biology

Expression

Bacterial

Mutation

Encodes for a full deletion of T7gp1.7

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pOPS0381

Plasmid

#133231

Purpose

Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

Depositor

Philip Poole

Article

Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

constructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1-HiBiT

Plasmid

#198310

Purpose

Loads HiBiT-tagged Pdp1 into PVCs; for payload loading assays; also contains Pnf

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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