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31,065 results

Showing: 6181 - 6200 of 31065 results

  2. MSP2292

    Plasmid
    #70707
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available Since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    70707-plasmid-map-sequence-id-127770

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  8. MT1G

    Plasmid
    #105704
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 1g
    Depositor
    Artur Krężel
    Insert
    Metallothionein 1g (MT1G Human)
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Available Since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    105704-plasmid-map-sequence-id-203324

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  9. pMSR

    Plasmid
    #161983
    Purpose
    Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile 630. Uses inducible expression of CD2517.1 toxin as a counter-selection marker
    Depositor
    Olga Soutourina
    Article
    Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi:
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Oct. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    161983-plasmid-map-sequence-id-321920

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  18. pPEPZ-sgRNAclone

    Plasmid
    #141090
    Purpose
    This vector is designed for efficient cloning of sgRNAs by Golden Gate assembly. The sgRNA insertion leads to replacement of mCherry, resulting in loss of red color of E. coli colony.
    Depositor
    Jan-Willem Veening
    Article
    Liu et al Cell Host Microbe. 2021 Jan 13;29(1):107
    Insert
    mCherry
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    p3
    Available Since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    141090-plasmid-map-sequence-id-330475

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  19. SHMT2

    Plasmid
    #25479
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SHMT2 (SHMT2 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    A269T
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25479-plasmid-map-sequence-id-168679

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Showing: 6181 - 6200 of 31065 results