30,307 results

1. pCDF_LuxR

   Plasmid

   #140039

   Purpose

   Constutitive expression of LuxR protein.

   Depositor

   Yolanda Schaerli

   Article

   Barbier et al Mol Syst Biol. 2020 Jun;16(6):e9361. doi

   Insert

   pLuxL and LuxR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MS2_SC2_S_Delta

   Plasmid

   #175950

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)

   Depositor

   Paul Freemont

   Inserts

   Maturation Protein
   Coat Protein Dimer
   S gene (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Delta (Amino Acid 319-869)

   Promoter

   T7

   Available since

   Oct. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pET28b-HRASLS3-1-132

   Plasmid

   #100725

   Purpose

   Expression of N-terminal region of human HRASLS3 in E.coli

   Depositor

   Byung Il Lee

   Article

   Han et al Protein Expr Purif. 2010 May;71(1):103-7

   Insert

   HRASLS3 (PLAAT3 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEDJ333

   Plasmid

   #177272

   Purpose

   GAL1p:Cas9:CYC1t

   Depositor

   Michael Jensen

   Article

   Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJM251 (miniCTX1-araC-ParaBAD)

   Plasmid

   #110567

   Purpose

   Integration vector for Pseudomonas aeruginosa with araC-ParaBAD inducible promoter

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   araC-ParaBAD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araC-ParaBAD

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTU424

   Plasmid

   #178983

   Purpose

   pACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)

   Depositor

   Stan Brouns

   Article

   van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

   Insert

   TPR-CHAT
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. 6BHD (SETDB1)

   Plasmid

   #119955

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   SETDB1 (SETDB1 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 190-410

   Promoter

   T7

   Available since

   Feb. 1, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. mEmerald-pBAD

   Plasmid

   #54220

   Purpose

   Localization: Protein Expression Vector , Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   6xHis and mEmerald

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

9. Terminase_T4

   Plasmid

   #188857

   Purpose

   Large terminase from phage T4

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

   Insert

   Terminase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 4R3H

    Plasmid

    #64652

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    YTHDC1:JMC092-F09:C231531 (YTHDC1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available since

    April 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

11. Dual opposing T7 promoters reporter plasmid

    Plasmid

    #156457

    Purpose

    A mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Insert

    NeoR/KanR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    Em7

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pASR103

    Plasmid

    #122637

    Purpose

    BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)

    Depositor

    Jan-Willem Veening

    Article

    Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. BRD3

    Plasmid

    #38940

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BRD3 (BRD3 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. ALIX-His(1-868, P801G)

    Plasmid

    #186808

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    ALIX-His (1-868, P801G) (PDCD6IP Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Proline 801 to Glycine

    Promoter

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. ScSpe1 pET-20b(+)

    Plasmid

    #117145

    Purpose

    Expresses the His tagged Saccharomyces cerevisiae Ornithine Decarboxylase in Escherichia coli BL21

    Depositor

    Markus Ralser

    Article

    Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

    Insert

    SPE1
    

    Use

    Tags

    Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTrcHisA-PPARalpha(aa101-468)

    Plasmid

    #13574

    Depositor

    Martin Scanlon

    Insert

    human peroxisome proliferator activated receptor alpha A/B domain deletion construct (aa101-468) (PPARA Human)
    

    Use

    Tags

    x6His N-terminal, enterokinase cleavage site

    Expression

    Bacterial

    Mutation

    deleted residues 1-100.

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21a-hAIM2-His6

    Plasmid

    #51532

    Purpose

    Express His6-tagged hAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    lac operator

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET22b-mEB1-eGFP-6xHis

    Plasmid

    #130981

    Purpose

    Bacterial expression of mouse EB1-GFP-6xHis for protein purification.

    Depositor

    Anne Straube

    Article

    Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

    Insert

    EB1-GFP-6xHis (Mapre1 Mouse)
    

    Use

    Tags

    eGFP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBSKrnaSeph

    Plasmid

    #67506

    Purpose

    Express the tRNALys scaffold with the sephadex aptamer tag

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

    Insert

    modified tRNALys with sephadex aptamer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pJK407

    Plasmid

    #72421

    Purpose

    Produces Acetobacter aceti 1023 Est06, C-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    lipase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    lacks Met1 and Thr2 in annotated (v1) sequence

    Promoter

    T7

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge