32,167 results
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Plasmid#62938PurposeExpression of (+36)GFP-GGS9-Cre-6xHis in bacterial cellsDepositorInsert(ps36)GFP-9xGGS-Cre
Tags(pos36)GFP and 6xHis tagExpressionBacterialPromoterT7Available SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2B-57
Plasmid#177927PurposeExpresses yeGFP under control of doxycycline-responsive TET3 promoter (contains three rtTA binding sites)DepositorArticleInsertEGFP
ExpressionBacterial and YeastPromoterTET3 (contains three rtTA binding sites)Available SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
KLHL11
Plasmid#39019PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXPR-SF61
Plasmid#163916PurposetRNA synthetase/tRNA pair for the in vivo incorporation of para-Pentafluorosulfanyl-Phenylalanine, into proteins in E. coli in response to the amber (TAG) codonDepositorInsertSF5Phe tRNA synthetase
ExpressionBacterialPromoteraraBADAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP5537
Plasmid#128730PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInsertHopAO1
ExpressionBacterialAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNP1 eforRed toehold switch
Plasmid#107353PurposeExpresses eforRed (an RFP derivative) in Ecoli off a T7 promoter when pNP1 eforRed trigger is co-expressedDepositorInserttoehold switch 1 + eforRed
ExpressionBacterialPromoterT7Available SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-p40R57Q PX
Plasmid#19009DepositorInsertp40phox (NCF4 Human)
TagsGSTExpressionBacterialMutationaa1-148 (PX domains) site directed mutagenesis: …Available SinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-154
Plasmid#118536PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmOrange2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4
Plasmid#87733PurposeBacterial expression of full-length S. cerevisiae Vps4DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
Tags6xHis-V5-TEVExpressionBacterialPromoterT7Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBTK564
Plasmid#110613PurposeBTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by PA1 promoterDepositorInsertNanoluc
ExpressionBacterialAvailable SinceAug. 10, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD/HisB-PhoCl-MBP
Plasmid#87689PurposeExpress PhoCl-MBP in E.coliDepositorInsertPhoCl fused to Maltose-Binding Protein
ExpressionBacterialAvailable SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
CFP-FRBx5-His
Plasmid#103784Purposeexpresses His-tagged iPOLYMER component in E. coli for protein purificationDepositorInsertCFP-FRBx5-His
ExpressionBacterialPromoterT7Available SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET14b PHAS-I
Plasmid#15679DepositorInsertPHAS-I (Eif4ebp1 Rat)
Tags6xHisExpressionBacterialMutationNucleotides 1-354 of rat PHAS-I plus 450bp of 3…Available SinceSept. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-Stx3(1-265)
Plasmid#100573PurposeExpresses cytoplasmic domain of Stx3(1-265) with a GST tag on the N-term.DepositorInsertStx3-1-265 (Stx3 Rat)
TagsGSTExpressionBacterialMutationNo transmembrane domain, residues 4-264 present.PromotertacAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFAB808
Plasmid#47853PurposeBIOFAB reporter plasmid for measuring rrnB T1 termination efficiencyDepositorInsertrrnB T1 terminator
UseSynthetic BiologyExpressionBacterialPromoterpLTetO8Available SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCol1a1-frt (FVB)
Plasmid#63575PurposeTargeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB.DepositorInsertCol1A-frt-hygro-pA
UseMouse Targeting; Frt/flpeExpressionBacterial and MammalianPromoterNoneAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mMaple3-kanMX6
Plasmid#105165PurposeYeast gene targetingDepositorInsertmMaple3-kanMX6
TagsGlySerGly-mMaple3ExpressionBacterial and YeastAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
MERTK_HUMAN_D0
Plasmid#79705PurposeThis plasmid encodes the kinase domain of MERTK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Top7 V48V
Plasmid#12393DepositorInsertTop7 V48V
Tags6xHisExpressionBacterialAvailable SinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pZ001 (AacC2c1 locus)
Plasmid#70166PurposeExpresses AacC2c1 full locusDepositorInsertAacC2c1 locus
ExpressionBacterialAvailable SinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB
Plasmid#110547PurposeCRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.DepositorInsertdCas9
ExpressionBacterialMutationD10A, H840APromoterpBADAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBP-C_ccdB
Plasmid#204114PurposeType 0 CDS partDepositorInsertToxic DNA gyrase inhibitor ccdB
ExpressionBacterialMutationNoneAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B split mCherry N-link/link-C
Plasmid#61965PurposeNegative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)DepositorInsertsN-mCherry-link
link-C-mCherry
ExpressionBacterialPromoterPBAD and PLtetOAvailable SinceFeb. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS6-GST-TEV-GG
Plasmid#215801PurposeCloning of target gene with N terminal His6 GST tag and TEV cleavageDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-6P1-Ataxin3Q22
Plasmid#22117DepositorAvailable SinceNov. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAtCHYb1Δ129N
Plasmid#53367PurposeContains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the product of which converts beta-carotene primarily to monocyclic beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi.DepositorInsertchyB1 (BETA-OHASE 1 Mustard Weed)
ExpressionBacterialMutationDeleted codons for the first 129 amino acidsPromoterTrcAvailable SinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltaCTD
Plasmid#157871Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal domainDepositorAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHS1398
Plasmid#205971PurposeMWEE01000013 Cas5-HNH crRNA expression in pCOLADuet-1DepositorInsertCas5-HNH crRNA
ExpressionBacterialPromoterT7Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS mouse c-Myc
Plasmid#14972DepositorAvailable SinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
L1-1180
Plasmid#13265DepositorAvailable SinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only