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Bacterial Expression

30,733 results

Showing: 641 - 660 of 30733 results

  1. pHN764

    Plasmid
    #178819
    Purpose
    Expression of EndoH in E. coli
    Depositor
    Kazuhiro Abe
    Insert
    synthetic gene encoding endo-β-N-acetylglucosaminidase H
    Tags
    HIs8 and MBP
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178819-plasmid-map-sequence-id-351934

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  7. MZB211

    Plasmid
    #228494
    Purpose
    10xHis-SUMO (Smt3) tagged Ssod7-fused Pfu Polymerase with fidelity enhancing mutation (E. coli based protein expression)
    Depositor
    Richard Baker
    Insert
    pol
    Tags
    10xHis-SUMO(Smt3)
    Expression
    Bacterial
    Mutation
    A408S
    Promoter
    T7 Promoter
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228494-plasmid-map-sequence-id-455979

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  8. prhaBAD-pA-Hia5

    Plasmid
    #222303
    Purpose
    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    pA-Hia5
    Tags
    6His and protein A
    Expression
    Bacterial
    Mutation
    codon optimized for bacterial expression
    Promoter
    rhaBAD
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222303-plasmid-map-sequence-id-447746

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  10. pEVOL-pAzF[TAG]

    Plasmid
    #164579
    Purpose
    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons
    Depositor
    Ryan Mehl
    Article
    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2
    Inserts
    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    araBAD, glnS, and proK
    Available Since
    May 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    164579-plasmid-map-sequence-id-392918

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  14. pTCF48 HLA-A*11:01

    Plasmid
    #180449
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*11:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    Feb. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    180449-plasmid-map-sequence-id-355113

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  20. pMM178

    Plasmid
    #61270
    Purpose
    Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    pLtetO, native, native (respectively)
    Available Since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61270-plasmid-map-sequence-id-99577

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Showing: 641 - 660 of 30733 results