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31,879 results

Showing: 641 - 660 of 31879 results

  4. pDM068

    Plasmid
    #216811
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216811-plasmid-map-sequence-id-430142

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  7. pTO149_RFP_CauNEO

    Plasmid
    #177277
    Purpose
    Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.
    Depositor
    Teresa O'Meara
    Article
    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:
    Insert
    RFP, CauNEO
    Tags
    ENO1-RFP
    Expression
    Bacterial
    Mutation
    All CUG Leucine codons were replaced with the syn…
    Promoter
    AgTEF2
    Available Since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    177277-plasmid-map-sequence-id-347241

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  9. pDM030

    Plasmid
    #216810
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and ble selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216810-plasmid-map-sequence-id-430137

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  10. pALS2-sfGFP 150TAG

    Plasmid
    #197574
    Purpose
    Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP 150TAG
    M. alvus Pyl-tRNA (6)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    araC and lpp
    Available Since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197574-plasmid-map-sequence-id-388126

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  11. His6-pAG-RTase

    Plasmid
    #214932
    Purpose
    Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems
    Depositor
    Chuan He
    Article
    Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s
    Insert
    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…
    Promoter
    T7 promoter
    Available Since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    214932-plasmid-map-sequence-id-426479

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  15. pET-Impact-T4gp41

    Plasmid
    #228207
    Purpose
    Expression in BL21(DE3) of T4 helicase
    Depositor
    Stephen Benkovic
    Insert
    41 (41 Escherichia phage T4)
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228207-plasmid-map-sequence-id-453945

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  16. pET-Impact-T4gp59

    Plasmid
    #228206
    Purpose
    Expression in BL21(DE3) of WT T4 helicase loader
    Depositor
    Stephen Benkovic
    Insert
    59 (59 Escherichia phage T4)
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228206-plasmid-map-sequence-id-453944

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  18. pHN924

    Plasmid
    #178885
    Purpose
    Expression of GST-His8-HRV3C
    Depositor
    Kazuhiro Abe
    Insert
    human rhinovirus 3C protease
    Tags
    GST, His8
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    178885-plasmid-map-sequence-id-354971

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Showing: 641 - 660 of 31879 results