30,256 results

1. pCas3cRh

   Plasmid

   #133773

   Purpose

   Expresses all necessary components of Type I-C CRISPR-Cas system

   Depositor

   Joseph Bondy-Denomy

   Article

   Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

   Inserts

   RhaR (rhaR )
   RhaS (rhaS )
   pRhaBAD
   I-C crRNA
   Cas3
   Cas5
   Cas8
   Cas7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKM208

   Plasmid

   #13077

   Depositor

   Kenan Murphy

   Article

   Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

   Insert

   lacI repressor (lacI E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAIDA1

   Plasmid

   #79180

   Purpose

   A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags

   Depositor

   Gen Larsson

   Article

   Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi

   Insert

   Adhesin Involved in Diffuse Adherence
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBT1-proD-mCherry

   Plasmid

   #65823

   Purpose

   Const. mCherry expression , proD promoter, E. coli Expression

   Depositor

   Michael Lynch

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   proD E . coli Constitutive Promoter

   Available since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21a-Streptavidin-Dead

   Plasmid

   #20859

   Purpose

   Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).

   Depositor

   Alice Ting

   Article

   Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

   Insert

   Streptavidin-Dead
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Triple mutant of wt Streptavidin N23A,S27D,S45A

   Promoter

   Available since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTECH-chAcK3RS(IPYE)

   Plasmid

   #104069

   Purpose

   Expression plasmid encoding chAcK3RS(IPYE) and pylT

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Plpp chAcK3RS(IPYE); PproK pyl
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains activating mutations V31I, T56P, H62Y, A…

   Promoter

   Plpp and PproK

   Available since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19_CDC45_dTAG

   Plasmid

   #232733

   Purpose

   Generates CDC45 dTAG C ternimal knock-in cell lines as donor

   Depositor

   Junjie Chen

   Insert

   CDC45 (CDC45 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Synonymous mutation

   Promoter

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX459_MCM2_sgRNA

   Plasmid

   #232730

   Purpose

   Generates MCM2 dTAG C ternimal knock-in cell lines

   Depositor

   Junjie Chen

   Insert

   MCM2 (MCM2 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   NO

   Promoter

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459_GINS4_gRNA

   Plasmid

   #232734

   Purpose

   Generates GINS4 dTAG C ternimal knock-in cell lines

   Depositor

   Junjie Chen

   Insert

   GINS4 (GINS4 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   NO

   Promoter

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459_CDC45_sgRNA

    Plasmid

    #232732

    Purpose

    Generates CDC45 dTAG C ternimal knock-in cell lines

    Depositor

    Junjie Chen

    Insert

    CDC45 (CDC45 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    NO

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19_GINS4_dTAG

    Plasmid

    #232735

    Purpose

    Generates GINS4 dTAG C ternimal knock-in cell lines as donor

    Depositor

    Junjie Chen

    Insert

    GINS4 (GINS4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Synonymous mutation

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19_MCM2_dTAG

    Plasmid

    #232731

    Purpose

    Generates MCM2 dTAG C ternimal knock-in cell lines as donor

    Depositor

    Junjie Chen

    Insert

    MCM2 (MCM2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Synonymous mutation

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJL1-sfGFP

    Plasmid

    #102634

    Purpose

    In vitro expression of sfGFP from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

    Insert

    sfGFP
    

    Use

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21a-Traptavidin

    Plasmid

    #24753

    Depositor

    Mark Howarth

    Article

    Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

    Insert

    Traptavidin
    

    Use

    Tags

    His-6

    Expression

    Bacterial

    Mutation

    Streptavidin with 2 amino acid mutations: S52G an…

    Promoter

    Available since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC18

    Plasmid

    #50004

    Purpose

    pUC cloning vector

    Depositor

    Joachim Messing

    Article

    Norrander et al Gene. 1983 Dec;26(1):101-6.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMBP-MS2

    Plasmid

    #65104

    Purpose

    can be used to isolate RNA binding complexes

    Depositor

    Josep Vilardell

    Article

    Macias et al Mol Cell. 2008 Jun 20;30(6):732-42. doi:

    Insert

    MS2
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    double mutation (V75Q and A81G) that prevents oli…

    Promoter

    tac

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. mEGFP-NbALFA

    Plasmid

    #159986

    Purpose

    For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    mEGFP-NbALFA
    

    Use

    Tags

    8histidine-HRV3C protease cleavage site

    Expression

    Bacterial

    Mutation

    3GS linker between mEGFP and NbALFA

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-45b(+)-Tn5

    Plasmid

    #112112

    Purpose

    Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

    Depositor

    Frank Pugh

    Article

    Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

    Insert

    Tn5
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E54K E110K P242A L372P

    Promoter

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSIBR-Int4-Cas9-NT

    Plasmid

    #235166

    Purpose

    Expresses the SIBR-Int4-Cas9 system and a NT sgRNA

    Depositor

    Constantinos Patinios

    Article

    Della Valle et al bioRxiv 2024.11.24.625072; doi: 10.1101/

    Insert

    SIBR-Int4-Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. HP17_KO7

    Plasmid

    #120346

    Purpose

    Helper plasmid for production of phagemid ssDNA, based on M13KO7

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

    Insert

    Coding part of the M13KO7 helper phage genome
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    native phage promoters

    Available since

    Aug. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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