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31,561 results

Showing: 641 - 660 of 31561 results

  2. pHelper-CRISPRi-sgRNA

    Plasmid
    #221135
    Purpose
    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with dCas9 handle
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23119(SpeI)
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221135-plasmid-map-sequence-id-436077

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  5. pTA-Mob 2.0

    Plasmid
    #149662
    Purpose
    Conjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.
    Depositor
    Bogumil Karas
    Article
    Soltysiak et al Int J Mol Sci. 2019 Oct 21;20(20). pii:
    Type
    Empty backbone
    Use
    Synthetic Biology; Conjugation helper plasmid
    Expression
    Bacterial
    Available Since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    149662-plasmid-map-sequence-id-379005

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  7. eGFP-luciferase-C1

    Plasmid
    #163523
    Purpose
    Expresses both eGFP and firefly luciferase in mammalian cells
    Depositor
    Thomas Weber
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    eGFP
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163523-plasmid-map-sequence-id-321576

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  8. pCasSA

    Plasmid
    #98211
    Purpose
    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9
    Depositor
    Quanjiang Ji
    Article
    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available Since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    98211-plasmid-map-sequence-id-485072

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  9. pKAR2-Br512

    Plasmid
    #161875
    Purpose
    Expresses Br512 polymerase in E.coli
    Depositor
    Andrew Ellington
    Article
    Maranhao et al medRxiv
    Insert
    Modified DNA Polymerase I Large Fragment (Br512)
    Tags
    8x His tag; Villin Headpiece 47 amino acid (HP47)…
    Expression
    Bacterial
    Promoter
    T7, tetPA
    Available Since
    Nov. 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    161875-plasmid-map-sequence-id-316881

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  19. pEM526

    Plasmid
    #248740
    Purpose
    Transcriptional reporter backbone/entry vector with mGL and constitutive mScarlet-I3 in pNBU2 in pNBU2 integrative Bacteroides plasmid
    Depositor
    Carolina Tropini
    Insert
    PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-[GG insertion site]
    Expression
    Bacterial
    Available Since
    Jan. 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    248740-plasmid-map-sequence-id-496782

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Showing: 641 - 660 of 31561 results