32,934 results
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Plasmid#197102PurposeTo express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.DepositorInsertTyrRS variant, amber suppressor tRNA
ExpressionBacterialMutationGly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)Available SinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBR1.cas9-NT
Plasmid#197871PurposeConstitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)DepositorInsertstrancCRNA
Cas9
crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
ExpressionBacterialAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-21a-PE2-His
Plasmid#201973PurposeExpress His-tagged PE2 in bacteriaDepositorInsertPE2-HIS
TagsHis and c-myc NLSExpressionBacterialAvailable SinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
TS245_pNKI-2-13_EGFP-DPN7
Plasmid#200719Purposeproducing Methyl Tracer EGFP protein in bacteriaDepositorInserteGFP-DPN7
Tags6xHisExpressionBacterialAvailable SinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
SUMO_TwinStrep_Csx30
Plasmid#197611PurposeFor protein purification of Csx30DepositorInsertSUMO_TwinStrep_Csx30
ExpressionBacterialAvailable SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-RFP entry
Plasmid#192278PurposeEntry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.DepositorInsertRFP flanked by type V-M CRISPR repeat sequences
UseCRISPRExpressionBacterialPromoterPJ23119Available SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHG4
Plasmid#188252PurposeIntegrating plasmid contains the TG1 int/attP locus and the apramycin-resistance gene (aac(3)IV) for selectionDepositorTypeEmpty backboneExpressionBacterialPromoteract1p/actII-orf4Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
His6_MBP_FXR1P
Plasmid#198694PurposeExpression of human FXR1 protein in E. coliDepositorAvailable SinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF-CjPglB
Plasmid#199305PurposepSN18 derivative encoding C.jejuni PglB with C-terminal FLAG epitope tagDepositorInsertCjPglB with flag tag
TagsFlag tagExpressionBacterialPromoterpBADAvailable SinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAKgfplux3
Plasmid#199304PurposeFluorescence and bioluminescence expression in Gram-negative bacteriaDepositorInsertChloramphenicol acetyltransferase
ExpressionBacterialPromoterT7Available SinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
luxB:Venus+luxA/pRSETB Duet
Plasmid#197582PurposeBRET lux luciferase with Venus for bacterial expressionDepositorInsertLuxA LuxBVenus
UseE.coli expressionTagsHis X 6ExpressionBacterialPromoterT7Available SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-Lck-GFP
Plasmid#177333PurposeExpresses GFP on the plasma membrane of neurons via an Lck localization sequenceDepositorInsertLck-GFP
UseAAVTagsGFPExpressionBacterialPromoterhSyn1Available SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Glt1-eGFP
Plasmid#176862PurposeExpresses Glt-1 fused with GFP at the C-terminus in astrocytesDepositorInsertGlt1-eGFP
UseAAVTagsGFPExpressionBacterialPromotergfaABC1DAvailable SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-LifeAct-GFP
Plasmid#190199PurposeExpresses the small actin binding peptide, LifeAct, with a GFP reporterDepositorInsertLifeAct-eGFP
UseAAVTagsGFPExpressionBacterial and MammalianPromotergfaABC1DAvailable SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-Ad5RGDPK7_Δpvc10
Plasmid#198449PurposeProduces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions); also contains truncated spike tip protein (Pvc10).DepositorInsertPVCpnf_pvc13-Ad5RGDPK7_Δpvc10
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-antiHistoneNb
Plasmid#198293PurposeProduces PVCs retargeted with A9 anti-histone nanobodyDepositorInsertPVCpnf_pvc13-antiHistoneNb
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-WWP2
Plasmid#186807PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-WWP2.DepositorAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDx_mScarlet-I3-NCwt-SYFP2
Plasmid#189765PurposeDual expression vector for bacteria and mammalian cells producing mScarlet-I3-NCwt-SYFP2 fusion protein for FRET analysisDepositorInsertmScarlet-I3-NCwt-SYFP2
Tags6xHisExpressionBacterial and MammalianPromoterCMV & RhaAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pHMattR3-attR4-GFP
Plasmid#196247PurposepHM1 derived plasmid containing the Gateway cassette attR3-attR4 and GFP geneDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only