30,327 results
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Plasmid#112780PurposeEncodes catechol-2,3-dioxygenase, from Pseudomonas putida TOL pWW0.DepositorArticleInsertxylE open reading frame
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOGG005
Plasmid#113980PurposeLevel 1 high copy number vector for E. coli expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)
Plasmid#114069PurposeT7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tagDepositorInserthuman codon optimized AsCas12a with C-terminal NLS and His-tag
UseTagsNLS(nucleoplasmin) and 6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
SNRPA_Full-length_pGEX
Plasmid#135134PurposeRecombinant expression of SNRPA with dual-affinity tagDepositorInsertSNRPA (SNRPA Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceFeb. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pBbB8k-csg-amylase
Plasmid#166859PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to α-amylase via a flexible linker. Derived from https://www.addgene.org/35363.DepositorInsertcsgBACEFG, with α-amylase fused to CsgA via flexible linker
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSKB-
Plasmid#62540PurposeCloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate CloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-tetA-Ptet-Rtet
Plasmid#189855PurposeCre-inducible activation of tetA for tetracycline resistance.DepositorInsertPtet-loxP-TT-loxP-Rtet-tetA
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DNAJB1_DNAJ
Plasmid#109918PurposeProtein expression and purification of DNAJB1_DNAJDepositorInsertDNAJB1_DNAJ
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJL1-mTagBFP2
Plasmid#102638PurposeIn vitro expression of mTagBFP2 from the T7 promoterDepositorInsertmTagBFP2
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-His6-UBCH7
Plasmid#212711PurposeBacterial expression of His6-UBCH7DepositorInsertUBCH7
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pSLC-255
Plasmid#124292PurposeExpression of vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.DepositorInsertvsfGFP-9
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Fusion of Dengue2 NS2B-NS3
Plasmid#204800PurposeBacterial expression of a fusion of Dengue2 NS2B-NS3 proteinsDepositorInsertDengue2 NS2B-NS3 fusion
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJUMP18-Histag_C
Plasmid#127035PurposeBasic Part C- C-terminus; polyhistidine tagDepositorInsertPart His6_C
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SARA-no stop
Plasmid#67347PurposehSara without stop codon in Gateway Entry vectorDepositorInsertSARA (SAR1B Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTU422
Plasmid#178982Purpose13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)DepositorInsertsgRAMP
CRISPR array
UseTagsStrep-Tag II SUMOExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG6s
Plasmid#62809PurposepPBC-LG6s expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6s under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP6s-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only