30,808 results

1. pWaldo-GFPe-SH1446

   Plasmid

   #112507

   Purpose

   Expresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.

   Depositor

   Simon Newstead

   Article

   Minhas et al Elife. 2018 Jul 3;7. pii: 34995. doi: 10

   Insert

   POT family transporter
   

   Tags

   C terminal TEV GFP octa-His affinity tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFE-Selo-FI-prk

   Plasmid

   #162698

   Purpose

   Expresses S. elongatus Form IB rubisco and S. elongatus Prk

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   S. elongatus Form IB rubisco and S. elongatus Prk
   

   Tags

   N terminal 6x His tag on PRK

   Expression

   Bacterial

   Promoter

   PLtet0-1 promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCB004

   Plasmid

   #134147

   Purpose

   Bacterial expression of human Ska3

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   human Ska3 (SKA3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. p7XNH3

   Plasmid

   #47064

   Purpose

   FX cloning E.coli expression vector with T7 promoter and N-terminal 10x His tag and 3C protease cleavage site

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Tags

   3C protease site (PreScission site) and decaHis (…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBBR1-kan-Ptet-plu-gamma-ET_rhi145

   Plasmid

   #180427

   Purpose

   Genome engineering of Agrobacterium

   Depositor

   Jun Fu

   Article

   Bian et al Appl Environ Microbiol. 2022 Mar 8;88(5)

   Insert

   plu-gamma-ET_rhi145
   

   Expression

   Bacterial

   Available Since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. UBC80

   Plasmid

   #25267

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   ubc80.229.304 (TSG101 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 13, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pCDH-EF1a-HygroR-T2A-TetOn3G

   Plasmid

   #179887

   Purpose

   Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   TetOn3G
   

   Use

   Lentiviral

   Tags

   Hygromycin resistance

   Expression

   Bacterial and Mammalian

   Promoter

   EF1a

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLS.491

   Plasmid

   #184956

   Purpose

   Test effect of a1/a2 length on editing rpoB using retron recombineering

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12
   

   Expression

   Bacterial

   Mutation

   rpoB donor T1534C

   Promoter

   T7/lac

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. p42.3A7

   Plasmid

   #69575

   Purpose

   OT-I TCR a chain cDNA in pCR1000

   Depositor

   Francis R. Carbone

   Article

   Hogquist et al Cell. 1994 Jan 14;76(1):17-27.

   Insert

   TCR alpha clone 149.42 (Tcra Mouse)
   

   Expression

   Bacterial

   Available Since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAH01Sp::Cas9D10A

    Plasmid

    #128161

    Purpose

    Ptet-controlled expression of S. pyogenes Cas9D10A variant

    Depositor

    Calvin Henard

    Article

    Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

    Insert

    Sp Cas9
    

    Expression

    Bacterial

    Mutation

    D10A

    Promoter

    Ptet

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXTK066 [Con-Pro_nptII-Broad_PartType8]

    Plasmid

    #229303

    Purpose

    XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_nptII-Broad_PartType8

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Insert

    nptII Promoter (Broad)
    

    Expression

    Bacterial

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBCG10

    Plasmid

    #232323

    Purpose

    Make mazF a counterselection marker to replace sacB in pZP06C

    Depositor

    Chenggang Wu

    Insert

    mazF-tetR
    

    Expression

    Bacterial

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. PCYT2A

    Plasmid

    #42415

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PCYT2A (PCYT2 Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. ScSpe1 pET-20b(+)

    Plasmid

    #117145

    Purpose

    Expresses the His tagged Saccharomyces cerevisiae Ornithine Decarboxylase in Escherichia coli BL21

    Depositor

    Markus Ralser

    Article

    Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

    Insert

    SPE1
    

    Tags

    Histidine

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-Sangamo-HIS

    Plasmid

    #40787

    Purpose

    for e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokI

    Depositor

    Gang Bao

    Insert

    Transcription Activator Like Effector Nuclease
    

    Use

    TALEN

    Tags

    C Terminal 6xHIS

    Expression

    Bacterial

    Mutation

    +63 C Terminus,Codon Optimized FokI Endonuclease

    Promoter

    T7

    Available Since

    Dec. 12, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pXZ13lacZalpha

    Plasmid

    #160229

    Purpose

    places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates

    Depositor

    Ian Duncan

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFosill-1

    Plasmid

    #89695

    Purpose

    to make Fosmids that can be converted into Illumina sequencable libraries

    Depositor

    Andreas Gnirke

    Article

    Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. JMJD5

    Plasmid

    #39113

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JMJD5 (KDM8 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

19. BRD3

    Plasmid

    #38940

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BRD3 (BRD3 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pETconNK

    Plasmid

    #81169

    Purpose

    pETconNK Empty Backbone

    Depositor

    Timothy Whitehead

    Article

    Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

    Type

    Empty backbone

    Tags

    c-myc

    Expression

    Bacterial and Yeast

    Promoter

    GAL1

    Available Since

    Feb. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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