30,327 results

1. pDropVn

   Plasmid

   #184873

   Purpose

   Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   lacZalpha,ccdB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. R725-X11-566: His6-MBP-tev-Hs.LGALS3

   Plasmid

   #159699

   Purpose

   E. coli protein expression of His6-MBP-tev-Hs.LGALS3

   Depositor

   Dominic Esposito

   Article

   Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

   Insert

   His6-MBP-tev-Hs.LGALS3 (LGALS3 Human)
   

   Use

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PGR-Blue

   Plasmid

   #68374

   Purpose

   Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.

   Depositor

   Nathan Reyna

   Type

   Empty backbone

   Use

   Synthetic Biology; Golden gate assembly

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFosill-2

   Plasmid

   #89696

   Purpose

   to make Fosmids that can be converted into Illumina sequencable libraries

   Depositor

   Andreas Gnirke

   Article

   Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9-beta

   Plasmid

   #169240

   Purpose

   Cas9 and beta expression plasmid for performing gene knockouts via oligo recombineering

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Inserts

   cas9
   beta
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBbB7k-GFP

   Plasmid

   #35360

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWT018a

   Plasmid

   #107885

   Purpose

   R3 plasmid. Contains PBAD-EGFP and is part of the CAMERA system for rewritable multi-event analog recording in bacteria

   Depositor

   David Liu

   Article

   Tang et al Science. 2018 Feb 15. pii: science.aap89

   Insert

   PBAD-EGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Csy4H29AS50C

   Plasmid

   #45029

   Depositor

   Jennifer Doudna

   Article

   Lee et al Proc Natl Acad Sci U S A. 2013 Apr 2;110

   Insert

   Csy4H29AS50C
   

   Use

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   H29A mutation to abolish catalytic activity and S…

   Promoter

   T7

   Available since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pLBH532_MBP-Cas14b1 expression

   Plasmid

   #112505

   Purpose

   Expresses 10xHis-MBP fused to Cas14b1 under t7 promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14b1
   

   Use

   CRISPR

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 6x-His_A1aY1_GFP

    Plasmid

    #158753

    Purpose

    Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

    Insert

    6xHistidine_A1aY1_GFP
    

    Use

    Tags

    6x-Histidine tag, GFP, T7 leader sequence, and Th…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKEW397_AcrIIA15

    Plasmid

    #138285

    Purpose

    Expression of AcrIIA15 protein

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA15
    

    Use

    CRISPR

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKEW394_AcrIIA14

    Plasmid

    #138283

    Purpose

    Expression of AcrIIA14 protein

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA14
    

    Use

    CRISPR

    Tags

    SFGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSA2

    Plasmid

    #52262

    Purpose

    Bacterial expression of human 6His-tagged SUMO2 and SUMO-E1

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-2 (SUMO2 Human)
    SAE-2 (UBA2 Human)
    SAE-1 (SAE1 Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. AURKA_HUMAN_D0

    Plasmid

    #79739

    Purpose

    This plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

15. RALA

    Plasmid

    #55657

    Purpose

    expression of mouse RALA

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    RALA (Rala Mouse)
    

    Use

    Tags

    GST and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-GST-AGO1

    Plasmid

    #24316

    Depositor

    Carl Novina

    Article

    Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

    Insert

    human AGO1 (AGO1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pMJ824

    Plasmid

    #39314

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    GST and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIYN3

    Plasmid

    #197099

    Purpose

    To express the variant of M. jannaschii TyrRS specific for halogenated tyrosines and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.

    Depositor

    Kensaku Sakamoto

    Article

    Mukai et al Biochem Biophys Res Commun. 2011 Aug 12;

    Insert

    TyrRS variant, Nap3 tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Ala70, Thr158, Ser159, Arg286 (TyrRS)

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWaldo-GFPd_PepTSt

    Plasmid

    #58333

    Purpose

    Expresses the POT family transporter from Streptococcus thermophilus in E. coli as a C-terminally tagged GFP his tagged protein

    Depositor

    Simon Newstead

    Article

    Solcan et al EMBO J. 2012 Aug 15;31(16):3411-21. doi:

    Insert

    PepTSt
    

    Use

    Tags

    GFP-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pEPSA5Cas9syn2

    Plasmid

    #206963

    Purpose

    S. aureus genome editing vector removed these two motifs AGGNNNNNGAT, CCAYNNNNNNTGT, CCAYNNNNNNGTA, GGANNNNNNNNTCG, and GWAGNNNNNNGAT

    Depositor

    Daijie Chen

    Article

    Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge