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pMRE-Tn7-161

Plasmid

#118573

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSMT-MjtRNA-GFP(47TAG)

Plasmid

#193251

Purpose

Expresses the M. jannaschii tRNATyr (MjtRNA) and GFP(47TAG) (aka GFP151TAG)

Depositor

Peter Schultz, Jessica Seeliger

Article

Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

Inserts

GFP(47TAG)
MjtRNA

Expression

Bacterial

Mutation

Codon for Tyr47 replaced with amber stop codon TA…

Promoter

hsp60 and metU

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMV361_MjTyrRS

Plasmid

#193250

Purpose

Expresses the M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS)

Depositor

Peter Schultz, Jessica Seeliger

Article

Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

Insert

MjTyrRS

Expression

Bacterial

Promoter

hsp60

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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H2BK120C

Plasmid

#75332

Purpose

Bacteria expression of H2BK120C

Depositor

Cynthia Wolberger

Article

Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

Insert

H2BK120C (h2bc11.S Frog)

Tags

none

Expression

Bacterial

Mutation

truncated; K120C

Promoter

T7

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pdCas9-J23111

Plasmid

#113149

Purpose

Plasmid constitutively expressing dCas9 protein

Depositor

Chong Zhang

Article

Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

Insert

dCas9

Expression

Bacterial

Mutation

D10A & H840A

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET-His-SCov2-nsp8

Plasmid

#154758

Purpose

Expression of SARS-CoV-2 nsp8

Depositor

Patrick Cramer

Article

Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

Insert

SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAD-sfGFP(Sp)-Pman

Plasmid

#133833

Purpose

Fluorescent protein sfGFP(Sp); under control of mannose inducible promoter.

Depositor

Oscar Kuipers

Article

Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

Insert

sfGFP(Sp)

Use

E.coli/bacillus spp. shuttle vector

Expression

Bacterial

Promoter

Pman

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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hDHFR

Plasmid

#184707

Purpose

bacterial expression of human DHFR

Depositor

Peter Wright

Article

Bhabha et al FEBS Lett. 2011 Nov 16;585(22):3528-32.

Insert

DHFR (DHFR Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_H1_alpha

Plasmid

#184865

Purpose

pSwap plasmid part with H1_alpha homology arms

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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IVT-MCP-Phi29

Plasmid

#207456

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

MCP-Phi29

Expression

Bacterial

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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