32,934 results
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Plasmid#109422PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and mycExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
B5-5
Plasmid#111484Purposetranslational initiation factor 3DepositorInserttranslational initiation factor 3
Tags6xHisExpressionBacterialAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Human RNF144A (16-228)
Plasmid#110752PurposeBacterial Expression of RNF144A RBR domainDepositorAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
Met1 diUb (1-152)
Plasmid#110766PurposeBacterial expression of Met1 diUbDepositorAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM343
Plasmid#71487PurposeContains loxP-hyg-cat-loxP cassette for recombineering; CamR, HygR.DepositorInsertloxP-hyg-cat-loxP
UseSource of loxp-hyg-cat- loxp for pcr amplificatio…ExpressionBacterialAvailable SinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP113 (pRS406-PRC1p-PRC1-GFP-ADH1t)
Plasmid#106464PurposeExpresses Prc1 with a C-terminal GFP tag in yeast cellsDepositorInsertPRC1
TagsGFPExpressionBacterial and YeastPromoterPRC1Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMP4639
Plasmid#107771PurposeExpression of EYFPDepositorInsertpLacZ-EYFP in pME6010
TagspLacZ-EYFPExpressionBacterialPromoterpLacZAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
HS-OS-Plk4-mKate2
Plasmid#105944Purposeheat shock inducible transgenesis, non phoshorylable version of Plk4DepositorInsertPlk4 (Plk4 Mouse)
TagsmKate2ExpressionBacterialMutation1002 to 3704 of Plk4/ deletion of S282-S305.Available SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-H3
Plasmid#109381PurposeArabinose inducible VioB-mCherry expression (weaker RBS). Used to test burden response in E. coliDepositorInsertpARABAD-VioB-mCherry
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDHC29
Plasmid#80781PurposeCloning vector compatible with ColE1 plasmids with high copy numberDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-b (p15A origin)
Plasmid#74089PurposeSecondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin
UseSynthetic BiologyExpressionBacterialPromoterNoneAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS52
Plasmid#104334PurposeExpresses sfGFP from the OmpR regulated PompF promoter.DepositorInsertsuperfolder GFP
ExpressionBacterialPromoterPompFAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GGG-HIS-CBM-Xmod-DocIII
Plasmid#91698PurposeCBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGGDepositorInsertGGG-HIS-CBM-Xmod-DocIII
TagsHIS-Tag and Sortase-TagExpressionBacterialMutationCysteine deletion in CBM C63SPromoterT7Available SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9HF1-indRecA56
Plasmid#102294PurposeModified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.DepositorInsertsCas9HF1
recA56
UseCRISPRExpressionBacterialMutationN497A/R661A/Q695A/Q926APromoterpTetAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mKikGR1 (mKikGR1-V69T)
Plasmid#99227PurposeBacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).DepositorInsertpr-mKikGR1
ExpressionBacterialMutationmonomeric variant with mutation V69T (Eos notatio…PromoterT7Available SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3 I0500 LasI
Plasmid#107966PurposeArabinose-inducible 3OC12HSL sender plasmidDepositorInsertlasI
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG_N2N3-HIS-ybbr
Plasmid#101238PurposeSdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilizationDepositorInsertSdrG_N2N3
TagsH3V 3C, HIS, and ybbrExpressionBacterialPromoterT7Available SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET_BGL1
Plasmid#101910PurposeExpresses a β-glucosidase gene from Thermomicrobium roseum in E. coli under the control of the pET system.DepositorInsertβ-glucosidase
UseSynthetic BiologyExpressionBacterialPromoterT7+LacOAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_FYN-1/1
Plasmid#91413PurposeProtein expression and purification of human SH3 domain construct FYN-1/1DepositorInsertFYN-1/1 (FYN Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-mEos3.2-A69T
Plasmid#98571Purposephotoconvertible fluorescent protein mEos3.2-A69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coliDepositorInsertmEos3.2-A69T
Tags7xHisExpressionBacterialPromoterT7Available SinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only