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pcDNA3-CMV-eBRIC

Plasmid

#241800

Purpose

Bioluminescence indicator for Ca2+ imaging in mammalian cells

Depositor

Huiwang Ai

Article

Tian et al ACS Sens. 2025 Jul 29. doi: 10.1021/acss

Insert

eBRIC

Expression

Bacterial

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pBad-HisB-RRvT

Plasmid

#87364

Purpose

Expresses RRvT, a tandem heterodimer red fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

RRvT

Tags

His-Tag

Expression

Bacterial

Promoter

pBad (Arabinose)

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB402-er-mCherry

Plasmid

#186288

Purpose

Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

er-mCherry

Expression

Bacterial

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOSIP-KP (KanR, Phi80)

Plasmid

#45986

Depositor

Drew Endy, Keith Shearwin

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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A1-LCD WT

Plasmid

#169713

Purpose

Bacterial expression of human hnRNPA1-A Low complexity domain including amino acids 186-320

Depositor

Tanja Mittag

Article

Martin et al Science. 2020 Feb 7;367(6478):694-699. d

Insert

hnRNPA1 (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

hnRNPA1-A Low complexity domain including amino a…

Promoter

T7

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSEVA221AID-T7RNAP

Plasmid

#167977

Purpose

Expresses fusion protein AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

AID-T7RNAP

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pQE60-RRF-His

Plasmid

#124132

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Ribosome recycling factor (frr Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-HaloTag-CNA35

Plasmid

#215457

Purpose

Encodes the bacterial collagen-binding protein CNA35 with N-terminal HaloTag.

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

CNA35

Tags

HaloTag

Expression

Bacterial

Mutation

Includes stop codon

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pBroad-T7RNAP-apraR

Plasmid

#199993

Purpose

T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) apraA

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

T7RNAP Circuit

Expression

Bacterial

Mutation

copFnull

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI

Plasmid

#116954

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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