32,934 results

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Hp MTAN

Plasmid

#69802

Purpose

Expression of H. pylori J99 methylthioadenosine nucleosidase

Depositor

Vern Schramm

Article

Wang et al Biochemistry. 2012 Sep 4;51(35):6892-4.

Insert

Methylthioadenosine nucleosidase

Tags

Histidine tag

Expression

Bacterial

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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NM-01

Plasmid

#69799

Purpose

Bacterial expression of Cas9 with His, MBP, and Strep tags

Depositor

Gerard Cagney

Insert

Cas9

Tags

HIS, MBP, and StrepII

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A) and Histidine 840 …

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only
pBS-UPRT

Plasmid

#69310

Purpose

expresses UPRT cDNA

Depositor

Chris Doe

Article

Gay et al Nat Protoc. 2014 Feb;9(2):410-20. doi: 1

Insert

UPRT cDNA

Tags

HA

Expression

Bacterial

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only
pBAD-rxRFP1.2

Plasmid

#67839

Purpose

redox-sensitive red fluorescent protein rxRFP1.2

Depositor

Huiwang Ai

Article

Fan et al Anal Bioanal Chem. 2016 Jan 12.

Insert

rxRFP1.2

Tags

His Tag

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARF6-V5-His6

Plasmid

#67415

Purpose

Expression of hArf6 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF6 (ARF6 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pLpp5oGFP

Plasmid

#69345

Purpose

Orthogonal GFP message under lpp5 promoter

Depositor

Michael Jewett

Article

Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

Insert

super folder GFP

Expression

Bacterial

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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p42.3A7

Plasmid

#69575

Purpose

OT-I TCR a chain cDNA in pCR1000

Depositor

Francis R. Carbone

Article

Hogquist et al Cell. 1994 Jan 14;76(1):17-27.

Insert

TCR alpha clone 149.42 (Tcra Mouse)

Expression

Bacterial

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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Kohinoor/pRSETb

Plasmid

#67770

Purpose

Kohinoor for bacterial expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pBAV-B

Plasmid

#65928

Purpose

Theophylline Riboswitch B with lacZ gene

Depositor

Justin Gallivan

Article

Topp et al Appl Environ Microbiol. 2010 Dec;76(23):

Insert

lacZ

Expression

Bacterial

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only
pBAV-A

Plasmid

#65927

Purpose

Theophylline Riboswitch A with lacZ gene

Depositor

Justin Gallivan

Article

Topp et al Appl Environ Microbiol. 2010 Dec;76(23):

Insert

lacZ

Expression

Bacterial

Promoter

T5

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only
pHis-zfCSF3b

Plasmid

#65613

Purpose

express soluble His-tagged zebrafish CSF3b/Gcsfb in E. coli

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

Insert

Csf3b (csf3b Zebrafish)

Tags

(His)6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only
130_pSKB3-LUMP-P.leioghnati

Plasmid

#65894

Purpose

Bacterial expression of P. leioghnati LUMP protein, a FRET acceptor protein emitting a cyan-colored fluorescence with an unusually long excited state lifetime

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Insert

LUMP

Expression

Bacterial

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pVHD

Plasmid

#61303

Purpose

Vanillate-inducible gene expression plasmid for Sphingomonas melonis Fr1; destination vector for Gateway cloning, allowing C-terminal fusion of proteins to HA tag

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Sci Rep. 2014 Sep 29;4:6453. doi: 10.103

Type

Empty backbone

Tags

HA

Expression

Bacterial

Promoter

PV10

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pETM6-P.accABCD

Plasmid

#66866

Purpose

Encodes accA, accB, accC, accD in Pseudo-operon configuration.

Depositor

Mattheos Koffas

Article

Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

Inserts

accA
accB
accC
accD

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK084

Plasmid

#65191

Purpose

Encodes KanR-ColE1 as a Type 8 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

KanR-ColE1

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK047

Plasmid

#65154

Purpose

Encodes GFP dropout as a Type 234r part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

GFP dropout

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pFGL822

Plasmid

#58225

Purpose

For fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.

Depositor

Naweed Naqvi

Article

Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

Insert

phosphinothricin acetyl transferase

Expression

Bacterial

Promoter

Aspergillus nidulans trpC promoter

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA5

Plasmid

#64985

Purpose

Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, P94R, D160N, D165A, K19…

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA

Plasmid

#64983

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Myt1

Plasmid

#64845

Purpose

A lentiviral vector for the expression of mouse Myt1 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Myt1 (Myt1 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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