30,808 results

1. pET-NtC60αESNtLC60β

   Plasmid

   #160878

   Purpose

   Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, and E. coli groES in E. coli.

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   rbcL, cpn60α, cpn60β, groES
   

   Expression

   Bacterial

   Promoter

   rbcL: T7, chaperones: T7

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-NtLNtC60αESC60β

   Plasmid

   #160875

   Purpose

   Expression of N. tabacum Rubisco large subunit (rbcL), Rubisco chaperone protiens cpn60α, cpn60β, and E. coli groES in E. coli

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   rbcL, cpn60α, cpn60β, groES
   

   Expression

   Bacterial

   Promoter

   rbcL: pBAD, chaperones: T7

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a(+) NRP1 b1b2 (273-586)

   Plasmid

   #162733

   Purpose

   Bacterial expression the b1b2 tandem domains from the rat Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

   Depositor

   Brett Collins

   Article

   Daly et al Science. 2020 Oct 20. pii: science.abd30

   Insert

   Human NRP1 b1b2 domains (residues 273-586)
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Codon optimised

   Available Since

   March 29, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pDD04neo pmyo2::gfp

   Plasmid

   #26393

   Depositor

   Denis Dupuy

   Article

   Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

   Type

   Empty backbone

   Expression

   Bacterial and Worm

   Available Since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-6P-1 Stu2 1-306

   Plasmid

   #38315

   Depositor

   Anthony Hyman

   Article

   Widlund et al Mol Biol Cell. 2012 Sep 19.

   Insert

   5' 918 bp from STU2 ORF (STU2 Budding Yeast)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX6P1-DEST-Strep2

   Plasmid

   #119753

   Purpose

   Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Strep-II tag.

   Depositor

   Andrew Jackson, Martin Reijns

   Type

   Empty backbone

   Tags

   GST and Strep-tag II fusion tag

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. P14-pZE-SUMO-ObiH

   Plasmid

   #204629

   Purpose

   ColE1 ori, KanR, TetR, Tet promoter with a codon optimized obiH gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.

   Depositor

   Aditya Kunjapur

   Article

   Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:

   Insert

   His-SUMO-TEV-ObiH
   

   Use

   Synthetic Biology

   Tags

   His Tag; SUMO Tag; TEV Protease Site

   Expression

   Bacterial

   Promoter

   PLtetO-1 promoter

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. SYKA-c020

   Plasmid

   #175489

   Purpose

   Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.

   Depositor

   Opher Gileadi

   Article

   Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

   Insert

   SYK (SYK Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHH0103_RANBP1_Ran-BP1

   Plasmid

   #109872

   Purpose

   Protein expression and purification of RANBP1_Ran-BP1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   RANBP1_Ran-BP1 (RANBP1 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-OptoPB

    Plasmid

    #113935

    Purpose

    Express a LOV2 fusion protein to enable light-dependent cytosol-to-PM translocation and dissect protein-phosphoinositide interactions

    Depositor

    Yubin Zhou

    Article

    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

    Insert

    OptoPB
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. LACTB2

    Plasmid

    #39115

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    LACTB2 (LACTB2 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pSucCD2

    Plasmid

    #83324

    Purpose

    expresses E. coli succinyl-CoA synthetase

    Depositor

    Robert Hausinger

    Insert

    sucCD
    

    Expression

    Bacterial

    Available Since

    Sept. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpyCatcher002-oHept

    Plasmid

    #124671

    Purpose

    Expresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oHept
    

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pB3.P11

    Plasmid

    #69577

    Purpose

    OT-I TCR beta chain cDNA in pCRScript

    Depositor

    Francis R. Carbone

    Article

    Hogquist et al Cell. 1994 Jan 14;76(1):17-27.

    Insert

    TCR beta cDNA clone B3 (Tcrb Mouse)
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOGG005

    Plasmid

    #113980

    Purpose

    Level 1 high copy number vector for E. coli expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pH6HTC_IGF2BP3-Halo

    Plasmid

    #175310

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    IGF2BP3 (IGF2BP3 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ftz

    Plasmid

    #11243

    Depositor

    Robin Reed

    Article

    Luo et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

    Insert

    Ftz (ftz Fly)
    

    Expression

    Bacterial

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pET16b-Trx CC32/35SS

    Plasmid

    #36946

    Depositor

    Anna Blom

    Article

    King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

    Insert

    Thioredoxin 1 (TXN Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    both active site cysteines (32,35) mutated to ser…

    Promoter

    T7

    Available Since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pHH0103_VDR_Hormone-recep

    Plasmid

    #109962

    Purpose

    Protein expression and purification of VDR_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    VDR_Hormone-recep
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMAL-EFSep

    Plasmid

    #34627

    Depositor

    Jesse Rinehart, Dieter Söll

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    EF-Sep (tufB E. coli)
    

    Tags

    6xHIS and MBP

    Expression

    Bacterial

    Mutation

    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

    Available Since

    March 1, 2012

    Availability

    Academic Institutions and Nonprofits only