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pET22-His-TEV-DOT1L

Plasmid

#89610

Purpose

Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416

Depositor

Leemor Joshua-Tor

Publication

Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

Insert

Dot1L (DOT1L Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCAS_gpyrG1

Plasmid

#90276

Purpose

(also pMST621-BB3_gpyrG1_cas9) CRISPR/Cas9 plasmid with gRNA for pyrG1, Cas9

Depositor

Michael Sauer

Publication

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

gRNA (pyrg1)

Use

A. niger

Expression

Bacterial

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBEST-p70a-UTR3-deGFP-6xHis-T500

Plasmid

#92224

Purpose

Expression of deGFP using the p70a promoter and UTR3

Depositor

Lital Alfonta

Publication

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

deGFP

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70a

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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PUC57_BAG3P209L-eGFP

Plasmid

#98183

Purpose

Codes for full length, GFP tagged mutant Bag3

Depositor

Robert Bryson-Richardson

Publication

Ruparelia et al Acta Neuropathol. 2014 Dec;128(6):821-33

Insert

BAG3P209L-eGFP (BAG3 Human)

Tags

GFP

Expression

Bacterial

Mutation

P209L

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b/BOA

Plasmid

#82041

Purpose

For E.coli expression of 6xHis-Tagged BOA (Burkholderia oklahomensis agglutinin)

Depositor

Angela Gronenborn

Publication

Whitley et al FEBS J. 2013 May;280(9):2056-67. doi: 10

Insert

Burkholderia oklahomensis agglutinin

Tags

6x His

Expression

Bacterial

Available since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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mEos3-caRac1

Plasmid

#92041

Purpose

Expresses mEos3 fusion with caRac1 (Q61L)

Depositor

Ji Yu

Publication

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RAC1 (RAC1 Human)

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Mutation

Q61L (constitutively active)

Promoter

CMV

Available since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEThT-CAHS3

Plasmid

#90028

Purpose

To express His-tagged CAHS3 in bacteria

Depositor

Takekazu Kunieda

Publication

Yamaguchi et al PLoS One. 2012;7(8):e44209.

Insert

CAHS3

Tags

His

Expression

Bacterial

Promoter

T7

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19-T7-SdB-T

Plasmid

#87307

Purpose

Expresses the fluorescence-activating Stabilized dimeric Broccoli (SdB) aptamer under the control of the T7 promoter.

Depositor

Donald Burke

Publication

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Stabilized dimeric Broccoli aptamer

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19-Split-Broccoli-Toehold-Switch

Plasmid

#87316

Purpose

Expresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.

Depositor

Donald Burke

Publication

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry

Use

Synthetic Biology

Expression

Bacterial

Promoter

P70a

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm_P1T_DP-B41

Plasmid

#90321

Purpose

Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS B41

Depositor

Andrew Goodman

Publication

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Type

Empty backbone

Expression

Bacterial

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pPBC-G6f

Plasmid

#62811

Purpose

pPBC-G6f expresses PiggyBac inverted terminal repeat-flanked GCaMP6f under the control of the CAG promoter.

Depositor

Karen Wilcox

Publication

Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

Insert

ITR-CAG-GCaMP6f-ITR

Expression

Bacterial and Mammalian

Promoter

CAG

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - SodA

Plasmid

#89612

Purpose

Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Publication

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Superoxide dismutase A

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - TR

Plasmid

#89621

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Publication

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Thioredoxin

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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N-BLa 1.1

Plasmid

#88994

Purpose

BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.

Depositor

Dieter Langosch

Publication

Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

Insert

N-BLa 1.1 fusion protein

Tags

Flag

Expression

Bacterial

Promoter

araBAD

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3(D3A: D175A D28A D9A)

Plasmid

#90090

Purpose

Expresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cells

Depositor

Clay Clark

Publication

Bose et al Biochemistry. 2003 Oct 28;42(42):12298-3

Insert

Caspase 3 (CASP3 Human)

Tags

His tag

Expression

Bacterial

Mutation

D175A D28A D9A

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET22b-csgB

Plasmid

#89582

Purpose

Express csgB curli protein with GS linker and Histaq

Depositor

Urartu Seker

Publication

Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

Insert

csgB (csgB )

Tags

3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…

Expression

Bacterial

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET30a-YedK

Plasmid

#86884

Purpose

Bacterial expression of E. coli SRAP ortholog YedK

Depositor

Douglas Feldman

Publication

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

YedK (yedK Eschericia coli)

Tags

6xHis, HA tag, and S-Tag

Expression

Bacterial

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe-PI(WT)-Rac1(WT)-mCherry

Plasmid

#87550

Purpose

PI(WT): Photo-inhibitable/blue light sensitive Rac1

Depositor

Klaus Hahn

Publication

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

LOV2
Rac1 (RAC1 Human)

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pYUB28b-MCS-optimized

Plasmid

#89179

Purpose

mycobacteria shuttle vector - pYUB28b with a single change to the MCS

Depositor

Ted Baker, Ghader Bashiri

Publication

Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

Type

Empty backbone

Use

Mycobacteria shuttle vector

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pYUB28b-MBP

Plasmid

#89178

Purpose

mycobacteria shuttle vector

Depositor

Ted Baker, Ghader Bashiri

Publication

Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

Type

Empty backbone

Use

Mycobacteria shuttle vector

Tags

His-MBP-TEV

Expression

Bacterial

Promoter

T7

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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