32,218 results
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Plasmid#40739PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceNov. 14, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits
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pMINTNH3
Plasmid#110074PurposeIntegrative vector for expression in mycobacteria based on the Tet promoter. Contains an N-terminal His10 Tag followed by a 3C cleavage siteDepositorTypeEmpty backboneTagsHis10 Tag - 3C cleavage siteExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET23-CBD-(-PP)-EGFP-H246,249D
Plasmid#12598DepositorTypeEmpty backboneTags6xHis and EGFPExpressionBacterialAvailable SinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCri_L889-antiSH2-OptoMB-Triple
Plasmid#159937PurposeOpto-MB bacterial expressionDepositorInsertanti-SH2 MB with SS58 insert
ExpressionBacterialMutationAsLOV2 V416I_G528A_N538EAvailable SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAQ482A
Plasmid#128945Purposeexpresses Q482A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHL 1075
Plasmid#53004PurposepCon:fhlA::mCherry, pLlacO-1:sodB::gfpDepositorInsertsfhlA
sodB
UseSynthetic BiologyTagsGFP and mCherryExpressionBacterialMutationcontains −309 to +96 bp relative to start codon a…PromoterpCon and pLlacO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJ2076.2_cL
Plasmid#140668PurposecL control for toggle switch experimentDepositorInsertcL control for toggle switch experiment
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP1171
Plasmid#119253Purposerfp "test" strainDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1170
Plasmid#119252Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-(CBX1)3-WT
Plasmid#180212PurposeBacterial expression of engineered CBX1 fusions with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1 x3 (CBX1 Human)
Tags6xhis-tag and MBPExpressionBacterialPromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHP-1(C)-SH2
Plasmid#46494DepositorInsertSHP-1(C) (PTPN6 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 105-217PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJMP1104
Plasmid#119248Purposeknockdown pqsC in P. aeruginosaDepositorInsertsgRNA pqsC (P. aeruginosa)
ExpressionBacterialMutationD10A and H840AAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCoofy12
Plasmid#43977DepositorTypeEmpty backboneTagsOneStrepExpressionBacterialPromoterT7Available SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBTBX-3
Plasmid#26069DepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-ZFP57
Plasmid#102716PurposeExpress DNA binding domain of human Zinc finger protein 57 in E. coliDepositorAvailable SinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCS-CyRFP1
Plasmid#84546PurposeBacterial expression of CyRFP1DepositorInsertCyRFP1
TagsHisExpressionBacterialAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH29_32 series plasmid
Plasmid#120901PurposeAlso known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated deviceDepositorInsertsEc-TTL-P109
sRNA-A targeting sequence
RBS TIR=974 of GFPop1 gene
GFPop1
RBS TIR=6474 of ECF32mut gene
ECF32mut
T7 terminator
resource competitor
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZE27GFP5
Plasmid#75458PurposePlasmid for constitutive expression of gfp-pdt5DepositorInsertgfp-pdt#5
ExpressionBacterialPromoterplacIq promoterAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b-EC12WTC9A
Plasmid#47924PurposeExpresses E-cadherin EC12 WT with C9A mutation in E.Coli.DepositorInsertE-Cadherin EC12
Tags6xHis and TEV CleavageExpressionBacterialMutationchanged Cysteine 9 to AlaninePromoterT7Available SinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRG/III-hs-MBP-T43NTR-TrxA-H10
Plasmid#121677PurposeBacterial expression of neurotensin receptor fusion proteinDepositorInsertneurotensin receptor (Ntsr1 Rat)
TagsTrxA-H10ExpressionBacterialMutationdeleted amino acids 1-42PromoterlacAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMINTC3GH (Kan)
Plasmid#110076PurposeAnalogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.DepositorTypeEmpty backboneTags3C cleavage site - GFP - His10 TagExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS/KS-mId3sp
Plasmid#16045DepositorAvailable SinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_VCP_AAA
Plasmid#109964PurposeProtein expression and purification of VCP_AAADepositorInsertVCP_AAA (VCP Human)
ExpressionBacterialAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1G1
Plasmid#97325PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1G1
Tags6xHis - TEVExpressionBacterialMutationAddition of G residue after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
R989-X11-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G13D
Plasmid#159543PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G13DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-169) G13D (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationG13DAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pExp-PpiB
Plasmid#112572PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis-PpiB and Strep tagExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAL-pgi_NT
Plasmid#74067PurposepAL374 plasmid carrying the pgi (NT) sgRNA, targeting the non-template strand of pgi, SpecRDepositorInsertsgRNA pgi (NT)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterptacAvailable SinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pED14xS-1
Plasmid#134800PurposeIPTG inducible expression of S-TAGA qtRNADepositorInsertS-TAGA qtRNA
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pED14xS-WT
Plasmid#134804PurposeIPTG inducible expression of WT S tRNA scaffold tRNADepositorInsertWildtype S tRNA (decodes TCG)
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJR2956
Plasmid#122533PurposeExpression of a C. elegan codon optimized fluorescent protein (CemOrange2) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemOrange2
ExpressionBacterialPromoterPnhx-2Available SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only