30,810 results

1. pDSG320

   Plasmid

   #115597

   Purpose

   surface-displayed Nb2, used in composability experiments; medium copy

   Depositor

   Ingmar Riedel-Kruse

   Article

   Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

   Insert

   regulated adhesin construct
   

   Expression

   Bacterial

   Available Since

   Oct. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINK-OTUD1 (OTU+UIM, aa 287-481)

   Plasmid

   #61406

   Purpose

   Expresses human OTUD1 (OTU+UIM domains) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD1 (OTUD1 Human)
   

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-286.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Dead Streptavidin-SpyTag

   Plasmid

   #59548

   Purpose

   pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

   Insert

   Dead Streptavidin-SpyTag
   

   Tags

   SpyTag

   Expression

   Bacterial

   Mutation

   The construct lacks final K of SpyTag

   Promoter

   T7

   Available Since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pPEPX-Plac

   Plasmid

   #122632

   Purpose

   BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae

   Depositor

   Jan-Willem Veening

   Article

   Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. SRF WT pGEX4T

   Plasmid

   #121100

   Purpose

   Expression of SRF as a GST fusion protein

   Depositor

   Paul Herring

   Article

   Herring et al J Biol Chem. 2001 Apr 27;276(17):14482-9

   Insert

   SRF (SRF Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. NSUN6

   Plasmid

   #188060

   Purpose

   Bacterial expression plasmid for human NSUN6; for activity testing

   Depositor

   Tanja Schirmeister

   Article

   Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

   Insert

   NSUN6 (NSUN6 Human)
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPEPY

   Plasmid

   #122633

   Purpose

   BglFusion compatible integration vector for Streptococcus pneumoniae

   Depositor

   Jan-Willem Veening

   Article

   Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. human p53-(1-360)

   Plasmid

   #24865

   Depositor

   Cheryl Arrowsmith

   Article

   Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

   Insert

   p53 (TP53 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains aa 1-360.

   Available Since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pLZL2386

   Plasmid

   #48682

   Purpose

   pET29a_PPP CODA PFV Integrase with C-terminal His tag (GPG removed from pLZL2373)

   Depositor

   Suzanne Sandmeyer

   Article

   Qi et al PLoS One. 2013 May 17;8(5):e63957. doi:

   Insert

   PFV Integrase
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. GEV 2

    Plasmid

    #12616

    Depositor

    John Louis

    Article

    Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

    Insert

    GB1 domain of protein G as N terminal tag, with IgT antigen as insert (SV40gp6 SV40)
    

    Tags

    G protein and His

    Expression

    Bacterial

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pDropVn

    Plasmid

    #184873

    Purpose

    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pET28b-HRASLS3-1-132

    Plasmid

    #100725

    Purpose

    Expression of N-terminal region of human HRASLS3 in E.coli

    Depositor

    Byung Il Lee

    Article

    Han et al Protein Expr Purif. 2010 May;71(1):103-7

    Insert

    HRASLS3 (PLAAT3 Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7 promotor

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYSD099

    Plasmid

    #212923

    Purpose

    A MoClo Yeast Toolkit LEU2, CEN/ARS empty vector (GFP dropout). Contains ConL1, part234 GFP dropout, ConR2, LEU2, CEN6/ARS4, AmpR-ColE1.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCA528 AMSH 1-178

    Plasmid

    #160050

    Purpose

    Expresses MIT domain (residues 1-178) of AMSH as a HIS-SUMO fusion protein. Internal ID: WISP18-22.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    AMSH residues 1-178 (STAMBP Human)
    

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-178

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOPINB-HOIL-1 helix-RBR (233–510)

    Plasmid

    #193859

    Purpose

    Expression of human His-tagged HOIL-1 (RBCK1) helix-RBR (aa 233–510) codon optimized for E. coli

    Depositor

    Bernhard Lechtenberg

    Article

    Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:

    Insert

    HOIL-1 (RBCK1 Human)
    

    Tags

    3C protease cleavage site and 6x-His tag

    Expression

    Bacterial

    Mutation

    Residues 233–510, codon optimised for expression …

    Promoter

    T7

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. KG#901

    Plasmid

    #110936

    Purpose

    Expresses Emerald-RAB-3 in the cholinergic motor neuron DA9 for imaging synaptic vesicles in a single neuron in a living animal

    Depositor

    Kenneth Miller

    Article

    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

    Inserts

    mig-13 promoter
    Emerald-RAB-3
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Expression

    Bacterial

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBbB0c-RFP

    Plasmid

    #35366

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. BB1_12_pGAP

    Plasmid

    #98498

    Purpose

    BB1 with insert: promoter PGAP

    Depositor

    Brigitte Gasser, Diethard Mattanovich, Michael Sauer

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Insert

    GAP promoter
    

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. 3521 pET15b Bid p15

    Plasmid

    #8782

    Depositor

    Stanley Korsmeyer

    Article

    Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

    Insert

    Bid p15 (Bid Mouse)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pGEX PLCg1(NC)-SH2

    Plasmid

    #46471

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    PLCg1(NC) (PLCG1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 542-759

    Promoter

    Tac

    Available Since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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