32,453 results
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Plasmid#74652PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPG1424PGA
TagsHis-6-TEVExpressionBacterialPromoterT7Available SinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9HF1-indRecA56
Plasmid#102294PurposeModified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.DepositorInsertsCas9HF1
recA56
UseCRISPRExpressionBacterialMutationN497A/R661A/Q695A/Q926APromoterpTetAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTrcHUM
Plasmid#19003DepositorInsertgamma-humulene synthase
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEN6-Ubc5 Sc
Plasmid#83234PurposepHis-par2-UbHs/Ubc5Sc/ Uba1TaDepositorAvailable SinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbS9a-RFP
Plasmid#126172PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterJ23113Available SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Nsp3 Mac1 - P43 (Oxford)
Plasmid#169210PurposeExpression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removalDepositorInsertSARS-CoV-2 Nsp3 Mac1
Tags6xHisExpressionBacterialPromoterT7Available SinceJuly 28, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CAGGS-EGFP-NLS-3XFLAG-V5
Plasmid#196090PurposeExpresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertGFP
TagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianPromoterCAGGsAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-luxKate
Plasmid#229946PurposeBacterial expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)DepositorInsertluxKate
TagsHis-tagExpressionBacterialPromoteraraBADAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFCiR5
Plasmid#149479PurposeBroad-Host range expression vector, RSF1010 origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertCinR(AM)-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPCinAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-MinD_MinE
Plasmid#133621PurposeExpression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.DepositorAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBL6
Plasmid#50549PurposeExpresses mCherry under the PLtetO-1 promoterDepositorInsertmCherry
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTCF10 HLA-B*08:01
Plasmid#180447PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-B*08:01
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHYRSF1-02
Plasmid#24765DepositorInsertNpuDnaE-IntC
TagsGB1 and HisExpressionBacterialAvailable SinceJune 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mYPet
Plasmid#103988PurposePlasmid encoding mYPetDepositorInsertmYPet
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F46L, I47L, …PromoterproCAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCK351
Plasmid#129696PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435DepositorInsertsPrhaBAD
yfp
rhaS
Terminator ECK120034435
ExpressionBacterialAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-ALFA
Plasmid#198301PurposeProduces PVCs retargeted with ALFA tagDepositorInsertPVCpnf_pvc13-ALFA
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPLN-hFluc-WT-HA-GFP11-KDEL
Plasmid#177722PurposeFirefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminusDepositorInserthFluc
TagsGFP11, HA, KDEL, and Prolactin Signal SequenceExpressionBacterial and MammalianAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ESRRA_Hormone-recep
Plasmid#109908PurposeProtein expression and purification of ESRRA_Hormone-recepDepositorInsertESRRA_Hormone-recep (ESRRA Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GAS7-1/1
Plasmid#91301PurposeProtein expression and purification of human SH3 domain construct GAS7-1/1DepositorInsertGAS7-1/1 (GAS7 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
p_SNIPR_sensor_GFP
Plasmid#139463PurposeT7 RNAP-driven expression of SNIPR RNA with a GFP-ASV reporterDepositorInsertSNIPR_GFP
ExpressionBacterialAvailable SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only