30,410 results

1. pCold-TF-hRubicon

   Plasmid

   #64147

   Purpose

   For protein expression in E. coli

   Depositor

   Tamotsu Yoshimori

   Article

   Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

   Insert

   Rubicon (RUBCN Human)
   

   Use

   Tags

   6xHis-tag and Trigger Factor (TF)

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pDEST17-UbE2A

   Plasmid

   #15780

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2A (UBE2A Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28b-ULP1-405-621

   Plasmid

   #229224

   Purpose

   For expression of ULP1 protein

   Depositor

   Andy LiWang

   Insert

   ULP1 (ULP1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   405-621 (truncated)

   Promoter

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFW1000

   Plasmid

   #224210

   Purpose

   E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVP91A-pcaHG

   Plasmid

   #113766

   Purpose

   Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.

   Depositor

   John Lipscomb

   Article

   Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11

   Inserts

   β subunit of Protocatechuate 3,4- Dioxygenase
   α subunit of Protocatechuate 3,4- Dioxygenase
   

   Use

   Tags

   Met-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T5 promoter

   Available Since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD/HisB-iRFP670

   Plasmid

   #45453

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP670
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

   Promoter

   araBAD

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-6P-1 SARS-CoV 3CLpro

   Plasmid

   #61692

   Purpose

   inducible expression of SARS 3CLpro protease

   Depositor

   Michael Johnson

   Article

   Lee et al Bioorg Med Chem. 2014 Jan 1;22(1):167-77

   Insert

   SARS 3CLpro protease
   

   Use

   Tags

   GST and HIS

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   April 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. 6xHis-MBP-huMb2Cas12a

   Plasmid

   #174676

   Purpose

   Expresses 6xHis-MBP tagged Mb2Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huMb2Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSF-T20S

   Plasmid

   #110805

   Purpose

   expresses Thermoplasma acidophilium 20S proteasome in E. Coli

   Depositor

   Yifan Cheng

   Article

   Yu et al Nat Commun. 2020 Sep 11;11(1):4580. doi:

   Inserts

   PsmB
   PsmA
   

   Use

   Tags

   tev-His

   Expression

   Bacterial

   Mutation

   N2D and Q2E

   Promoter

   T7 and T7 promoter

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. TOPO mCherry

    Plasmid

    #68490

    Purpose

    TOPO construct expressing mCherry for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    mCherry
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge