32,838 results
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Plasmid#146265PurposeBacterial Expression of PfAgoDepositorInsertPfAgo
ExpressionBacterialMutationone silent T1704C and one non silent mutation A90…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-PfAgo_F
Plasmid#146270PurposeBacterial Expression of PfAgoDepositorInsertPfAgo
ExpressionBacterialMutationone non silent mutation A907G (I303V) and one sil…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
P-BT1763
Plasmid#188111PurposepBolux harboring the promoter region upstream of BT1763DepositorInsertThe B. thetaiotaomicron BT1763 promoter cloned into pBolux
ExpressionBacterialPromoterBT1763Available SinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a-ncOGT-TPR
Plasmid#190822PurposeFor expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags6x HisExpressionBacterialMutationC467X. Also codon optimized for expression in E.c…PromoterT7Available SinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXD70CT5.1-PxylR-CymR, Pveg(2CuO)-LacZ
Plasmid#191628PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1 (tandem CymR binding sites)-LacZDepositorInsertlacZ
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70DA9-Pct5-DadhABC(A2)
Plasmid#191632PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strainDepositorInsertDopamine dehydroxylase
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
cr3Dd
Plasmid#184862Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination systemDepositorInsertCas9, bet, exo
TagsCas9 ssrA degradation tagExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-GpA-G83I
Plasmid#191239PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
Tagsnuclease A from S. aureus fused to the TM domain …ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and Glycin…PromoterT7 promoterAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-GpA
Plasmid#191238PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
Tagsnuclease A from S. aureus fused to the TM domain …ExpressionBacterialMutationChanged Alanine 112 to CysteinePromoterT7 promoterAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6
Plasmid#192788PurposeaGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labelingDepositorInsertantiGFP nanobody enhancer
TagsH6 and ybbRExpressionBacterialAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6
Plasmid#192789PurposeaGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labelingDepositorInsertantiGFP nanobody minimizer
TagsH6 and ybbRExpressionBacterialAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-TEV-AtPCO4-1(wt)
Plasmid#191801PurposeFull length wt AtPCO4 with Nt-His tagDepositorInsertAtPCO4-1
Tags6xHis, TEV cleavage siteExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_iRFP670
Plasmid#191749PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
TagsNCU04502 promoter and iRFP670ExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_vsfGFP9
Plasmid#191747PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
TagsNCU04502 promoter and vsfGFP-9ExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
csr-1_iRFP670_son-1_GpdToxA_bml_mTagBFP2
Plasmid#191751PurposeMicrotubule marker together with nuclear envelope marker driven by bidirectional promoterDepositorTagsGdp1 promoter, ToxA promoter, iRFP670, and mTagBF…ExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRnc-gGFP161
Plasmid#189536PurposeExpresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFPDepositorInsertsRNase III
gUTR-161-GFP
ExpressionBacterialMutationN/APromoterJ23119 and pBADAvailable SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCSG28-SH3-588
Plasmid#185920PurposeExpresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferaseDepositorInsertSH3-588
Tags6x HIS tagExpressionBacterialMutationDesigned enzyme with novel interface between huma…PromoterT7Available SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2-sfCherry2_SEPT6
Plasmid#180313Purposebacterial co-expression of human SEPT2 fused to superfolder Cherry2 and of human SEPT6DepositorTagsHis6-TEV and sfCherry2ExpressionBacterialAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-pHmGL
Plasmid#191257PurposeBacterial expression plasmids for pH sensitive fluorescent protein pHmGLDepositorInsertpHmGL
TagsHis tagExpressionBacterialAvailable SinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15A-YFP
Plasmid#186563Purposep15A ori, promotorless, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
TagsNoneExpressionBacterial and MammalianMutationNonePromoterPromotorlessAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQnl_Pcphy23
Plasmid#191354PurposeClostridium plasmid expressing NanoLuc reporter from moderate, constitutive promoterDepositorInsertPcphy23-NanoLuc
ExpressionBacterialPromoterPcphy23Available SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQmod2E-GG
Plasmid#191345PurposeClostridium expression vector (pBP1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
ExpressionBacterialPromoterPlacAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQmod3E-GG
Plasmid#191348PurposeClostridium expression vector (pCB102 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
ExpressionBacterialPromoterPlacAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQmod4E-GG
Plasmid#191351PurposeClostridium expression vector (pCD6 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
ExpressionBacterialPromoterPlacAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pATmin-GG
Plasmid#191352PurposeClostridium expression vector (pAMB1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
ExpressionBacterialPromoterPlacAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE065
Plasmid#176822PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert704
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE050
Plasmid#176807PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert601
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-SPW-RHH
Plasmid#190630PurposeBacterial expression of genetically encoded protease indicator SPW-RHHDepositorInsertSPW-RHH
ExpressionBacterialAvailable SinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnxABE-flgn
Plasmid#187932PurposeA-to-G base editorDepositorInsertecTadA(8e)-nxCas9 (D10A)
UseCRISPRExpressionBacterialAvailable SinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPgiCas13b
Plasmid#184833PurposeInducible expression of PgiCas13b nuclease in bacteria.DepositorInsertCas13b nuclease from Porphyromonas gingivalis
UseCRISPRExpressionBacterialPromoterT7 promoterAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only