32,218 results

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pT2-cryR;lmo2CE1-P1Egfp

Plasmid

#90153

Purpose

contains lmo2 enhancer sequence, and lmo2 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

lmo2E1P1Egfp (lmo2 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-MK3

Plasmid

#30188

Depositor

Bruce Tempel

Insert

Kcna3 (Kcna3 Mouse)

Expression

Bacterial

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pZE-P9-egfp

Plasmid

#201790

Purpose

the promoter can be recognized by PadR

Depositor

Yajun Yan

Article

Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

Insert

P9

Expression

Bacterial

Mutation

WT

Available Since

July 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pHH0103_LYN-1/1

Plasmid

#91536

Purpose

Protein expression and purification of human SH3 domain construct LYN-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

LYN-1/1 (LYN Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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Sup35 ScNM A230C His7

Plasmid

#15598

Depositor

Susan Lindquist

Article

Tessier et al Nature. 2007 May 31. 447(7144):556-61.

Insert

Sup35 NM (SUP35 Budding Yeast)

Tags

7xHis

Expression

Bacterial

Mutation

A230C. Alanine 230 mutated to cysteine.

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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PDLIM7

Plasmid

#38878

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PDLIM7 (PDLIM7 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pET15b-Opt12

Plasmid

#61697

Purpose

Expresses PTDH mutant Opt12

Depositor

Huimin Zhao

Article

McLachlan et al Biotechnol Bioeng. 2008 Feb 1;99(2):268-

Insert

Opt12

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2015

Availability

Academic Institutions and Nonprofits only
pETM30-ARC105

Plasmid

#133426

Purpose

Human ARC105 coding sequence in vector for in vitro transcription and protein expression, with T7 promoter.

Depositor

Gerhard Wagner

Article

Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

Insert

PCQAP (MED15 Human)

Expression

Bacterial

Mutation

Contains amino acids 5-78 fused to C-terminal of …

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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GST-U1C

Plasmid

#1840

Depositor

Philip Leder

Article

Bedford et al Proc Natl Acad Sci U S A 1998 Sep 1;95(1

Insert

U1C (SNRPC Human)

Tags

GST

Expression

Bacterial

Available Since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
pBAD-O-GECO1

Plasmid

#113938

Purpose

Bacterial expression of orange fluorescent Ca2+ sensor O-GECO1

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

O-GECO1

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMPMA3dPlac-PsopB-GFPLVA

Plasmid

#23341

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

Insert

GFP(LVA)

Tags

GFP(LVA)

Expression

Bacterial

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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Citrine-pBAD

Plasmid

#54772

Purpose

Localization: Protein Expression Vector , Excitation: 516, Emission: 529

Depositor

Robert Campbell, Michael Davidson, Oliver Griesbeck, Roger Tsien

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Type

Empty backbone

Tags

6xHis and Citrine

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pTP443

Plasmid

#216361

Purpose

xNup98-Nb1t (+1Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup98-Nb1t (+1Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pKLD-CAG-DYSF-CFL

Plasmid

#131457

Purpose

Encodes Dysferlin protein (Therapeutic gene) along with Follistatin protein to cure LGMD2B along with luciferase enzyme for in vivo live imaging

Depositor

Michele Calos

Article

Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

Insert

DYSF and Follistatin (DYSF, FST Human)

Expression

Bacterial and Mammalian

Mutation

R15C (in DYSF) and Q2579H (in CFL)

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pIOM17

Plasmid

#20870

Depositor

Carmen Beuzón

Article

Ortiz-Martin et al J Microbiol Methods. 2006 Dec . 67(3):39

Insert

aphA::cat

Expression

Bacterial

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pGEX6P1-GST-Laforin

Plasmid

#84673

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pETM30-C9ORF78_F8A

Plasmid

#193369

Purpose

Expression of C9ORF78 F8A variant in E.coli with N-terminal His6-GST-tag which is cleavable with TEV protease

Depositor

Markus Wahl

Article

Bergfort et al Nat Commun. 2022 Mar 3;13(1):1132. doi:

Insert

C9ORF78_F8A (C9orf78 Human)

Tags

His6-GST-TEV

Expression

Bacterial

Mutation

F8A, the mutation is located in the binding inter…

Promoter

T7

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ915v2+Nterm Spe1 +Cterm Age1 site

Plasmid

#88914

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pGEMT-Map7-1333-end

Plasmid

#46070

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7-1333-end (Map7 Mouse)

Expression

Bacterial

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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CHD1 (CHD1A-c107)

Plasmid

#110253

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

CHD1 (CHD1 Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7 - lacO

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE150

Plasmid

#118500

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-PP-Spastin(87-616)DeltaExon4

Plasmid

#128794

Purpose

Bacteiral expression of Spastin M87 varient without exon 4

Depositor

Chris Hill

Article

Han et al J Biol Chem. 2019 Nov 25. pii: AC119.009

Insert

Spastin (SPAST Human)

Use

`

Tags

GST

Expression

Bacterial

Mutation

residues 87-616 without exon 4.

Promoter

T7

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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4QQ6

Plasmid

#61946

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

SMN1:JMC047-F11:C221089 (SMN1 Human)

Tags

6xHis and MHHHHHHSSGRENLYFQG

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available Since

March 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pQZ083

Plasmid

#106417

Purpose

Express Complexin-1 fragment (1-83) in E. coli

Depositor

Axel Brunger

Article

Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

Insert

complexin-1 fragment 1-83 (Cplx1 Synthetic, Rat)

Tags

GST tag

Expression

Bacterial

Mutation

Fragment 1-83, Codon optimization

Promoter

T7

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;dll4CE1-P1Egfp

Plasmid

#90146

Purpose

contains dll4 enhancer 1 sequence, and dll4 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dll4E1P1Egfp (dll4 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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ptetmCherry_prey_plasmid-2

Plasmid

#34617

Depositor

Lingchong You

Article

Song et al Nat Chem Biol. 2009 Dec;5(12):929-35. Ep

Insert

mCherry

Expression

Bacterial

Promoter

ptet

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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pJR2955

Plasmid

#122550

Purpose

Expression of a C. elegan codon optimized florescent protein (CemNeptune2.5) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_CemNeptune2.5

Expression

Bacterial

Promoter

Pnhx-2

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJJS010

Plasmid

#188333

Purpose

PCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

Linker::mKate2::mIAA7

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJS004

Plasmid

#188327

Purpose

PCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

Linker::mCherry::mIAA7

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJGL004

Plasmid

#180608

Purpose

Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX+Region1 insertion

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Insert

PlmCasX with DpbCasX R1 loop

Use

CRISPR

Tags

10xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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