32,669 results
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Plasmid#48123PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promoterDepositorInsertsignal peptide of Pac
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NR3C2_Hormone-recep
Plasmid#109848PurposeProtein expression and purification of NR3C2_Hormone-recepDepositorInsertNR3C2_Hormone-recep (NR3C2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-SynTetOff-GFP
Plasmid#190230PurposeGateway entry vector, high-level expression of EGFP in neuronal cellsDepositorInsertEGFP
ExpressionBacterialAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEK19
Plasmid#210578PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdBIP1pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastPromoterBdBIP1proAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSG318
Plasmid#115595Purposesurface-displayed Ag2, used in composability experiments; medium copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
P70a-deGFP-sc101-kan
Plasmid#184841PurposeExpression of deGFPDepositorInsertgreen fluorescent protein
UseSynthetic BiologyExpressionBacterialPromoterpromoter OR2-OR1Available SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-BB0323-ss
Plasmid#137067PurposeE. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66700.1
TagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature BB0323: lacks the BB0323 signal peptidePromoterT7lacAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKD227
Plasmid#90954PurposeExpresses S. baltica TtrS under the Ptac promoterDepositorInsertTetrathionate sensor kinase
ExpressionBacterialAvailable SinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
PCRT7 tetR pLtetO MBP-MEK1 XX Amp
Plasmid#53225Purposehuman MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesisDepositorInsertMEK1 (MAP2K1 Human)
UsePhosphoprotein synthesisTagsHIS and MBPExpressionBacterialMutationCodon optimized for E.coli, both codons for Ser21…PromoterpLtetOAvailable SinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-8
Plasmid#172772PurposeBacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-8
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC0062 RanCas13 (B06) His6-TwinStrep-SUMO-BsaI
Plasmid#115213PurposeExpresses RanCas13b from a SUMO-tagged bacterial expression constructDepositorInsertRanCas13
TagsSUMOExpressionBacterialAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSSK p85 alpha (delta iSH2)
Plasmid#13432DepositorInsertp85 alpha (delta iSH2) (PIK3R1 Human)
TagsflagExpressionBacterialMutationDominant negative p85 alpha. The p110 alpha bindi…Available SinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAWPSG-Ptet-nCas9-BE
Plasmid#195739PurposeExpress APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coliDepositorArticleInsertTet repressor protein / APOBEC1-nCas9(D10A)-UGI
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPtet(Tetracycline inducible protein)Available SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGDP4 ermC
Plasmid#112884PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIOM15
Plasmid#20869DepositorInsertaphA::aadA
ExpressionBacterialAvailable SinceJuly 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCON1-ProD.gfp
Plasmid#112518PurposeProD driven gfpmut3 expression with synTTDepositorInsertProD gfpmut3 TT
ExpressionBacterialPromoterProDAvailable SinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPhlF-P3
Plasmid#74687PurposePhlF-P3 gateDepositorInsertPTac-RBSP3-PhlF-PPhlF-YFP
ExpressionBacterialAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30a-FluA-NP
Plasmid#165092Purposeprotein expression in E. coliDepositorInsertInfluenza A virus(A/Georgia/03/2019(H1N1) nucleocapsid protein
ExpressionBacterialAvailable SinceFeb. 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPbcsx28
Plasmid#89909PurposeExpresses csx28 from P. buccae ATCC 33574 in bacteria. Expression driven from the Lac promoterDepositorInsertcsx28
ExpressionBacterialPromoterLacAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -