30,875 results

1. pQCasTns(Ptr)-entry(BsaI)

   Plasmid

   #190274

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Insert

   PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(BsaI)
   

   Expression

   Bacterial

   Mutation

   no

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJUMP27-1A(sfGFP)

   Plasmid

   #126974

   Purpose

   Level 1 vector. sfGFP reporter. Origin pSC101 (low copy number)

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   None

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-GFP-AURKA

   Plasmid

   #99875

   Purpose

   Expression of GFP-AURKA for protein purification in bacteria

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   GFP

   Expression

   Bacterial

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEM-3XP3.RFP-tan

   Plasmid

   #83474

   Purpose

   RFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

   Depositor

   Patricia Wittkopp

   Article

   Lamb et al Fly (Austin). 2016 Aug 5:1-12.

   Insert

   3XP3.RFP-tan
   

   Use

   CRISPR

   Expression

   Bacterial and Insect

   Promoter

   3XP3 eye-expression Promoter

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJB007

   Plasmid

   #98995

   Purpose

   Express FtsI tagged with tagRFP-t in E coli

   Depositor

   Jie Xiao

   Article

   Yang et al Science. 2017 Feb 17;355(6326):744-747.

   Insert

   FtsI
   

   Tags

   TagRFP-T

   Expression

   Bacterial

   Promoter

   T5-lac

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBL132

   Plasmid

   #104333

   Purpose

   Expresses taz from a LacI regulated promoter under inducible IPTG control.

   Depositor

   Jeffrey Tabor

   Article

   Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

   Insert

   Taz
   

   Expression

   Bacterial

   Promoter

   PtacI

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDSG321

   Plasmid

   #115598

   Purpose

   surface-displayed Nb3, used in composability experiments; medium copy

   Depositor

   Ingmar Riedel-Kruse

   Article

   Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

   Insert

   regulated adhesin construct
   

   Expression

   Bacterial

   Available Since

   Oct. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIA1362

   Plasmid

   #166860

   Purpose

   Expression of codon optimized SARS-COV-2 Nsp7 in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

   Insert

   SARS-COV-2 Nsp7 (ORF1ab Synthetic)
   

   Tags

   His-GB1

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. USP21 (3I3T)

   Plasmid

   #25147

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   usp21.209.563 (USP21 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 6, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pLP-pTac-Tn5-chlorR

    Plasmid

    #199994

    Purpose

    Landing Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information.

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Landing Pads
    

    Expression

    Bacterial

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPath-pTac-integrase

    Plasmid

    #199999

    Purpose

    Entry vector for biosynthetic pathway. pTac-phiC31 integrase. This material contains 2 plasmids. For more information look under the "Depositor Comments" section below.

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Pathways
    

    Expression

    Bacterial

    Available Since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQlinkH-PTPIP51(36-235)

    Plasmid

    #170533

    Purpose

    Expresses Cleavable His-tagged PTPIP51_ΔTPR domain (36-235) in E. Coli

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T5 promoter

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTJV1ScKm-rpoB

    Plasmid

    #166980

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    NeoR/KanR
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-2T-RNAPII-CTD wt #14-HA

    Plasmid

    #160688

    Purpose

    Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

    Depositor

    Jeffry Corden

    Article

    Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

    Insert

    RNA Polymerase II CTD
    

    Expression

    Bacterial

    Mutation

    C terminal domain only

    Available Since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28b-FOXP2_P6

    Plasmid

    #122649

    Purpose

    Expresses truncated variant (aa271-aa500) of human FOXP2 in bacterial cells

    Depositor

    Hendrik Dietz

    Article

    Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

    Insert

    Homo sapiens forkhead box P2 (FOXP2 Human)
    

    Tags

    His-tag and Strep-tag II

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. EPB104

    Plasmid

    #68369

    Purpose

    contains human U6 promoter

    Depositor

    Eric-Paul Bennett

    Article

    Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

    Insert

    U6 promoter (RNU6-1 Human)
    

    Expression

    Bacterial

    Promoter

    U6

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. B7-1

    Plasmid

    #111489

    Purpose

    translational elongation factor G

    Depositor

    Cheemeng Tan

    Article

    Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

    Insert

    translational elongation factor G
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBabe-PI(WT)-Src(WT)-mCherry

    Plasmid

    #87356

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive ; WT: wild type

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Src (Src Mouse)
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCri-18a

    Plasmid

    #61326

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Tags

    His-tag

    Expression

    Bacterial

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

20. LB1353 (Bcl-2 promoter, no IgH enh)

    Plasmid

    #15393

    Depositor

    Linda Boxer

    Article

    Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

    Insert

    B-cell lymphoma/leukemia-2 (BCL2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Bacterial and Mammalian

    Mutation

    Bcl-2 with full promoter and no IgH enhancers MHS…

    Available Since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only