30,222 results

1. pCPP5588

   Plasmid

   #128708

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   hopD1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCPP5053

   Plasmid

   #128707

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   hopC1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCPP5530

   Plasmid

   #128705

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   hopA1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET30a-FluB-NP

   Plasmid

   #165093

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Influenza B virus(B/Georgia/05/2019) nucleocapsid protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 11, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pBS hBAF57

   Plasmid

   #17877

   Depositor

   Jerry Crabtree

   Article

   Chi et al Nature. 2002 Jul 11. 418(6894):195-9.

   Insert

   BAF57 (SMARCE1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pMAL-c4E

   Plasmid

   #75293

   Purpose

   Expresses proteins in the cytoplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

   Depositor

   Paul Riggs

   Article

   Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

   Type

   Empty backbone

   Use

   Tags

   maltose-binding protein

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 19, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. PERK 4: PERKKD-pGEX4T-1

   Plasmid

   #21817

   Depositor

   David Ron

   Article

   Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

   Insert

   PERK (537-1114) (Eif2ak3 Mouse)
   

   Use

   Tags

   GST and Myc (9E10)

   Expression

   Bacterial

   Mutation

   PERK Kinase Domain. Amino acids 537-1114.

   Promoter

   Available since

   March 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pH3U3-Zif268

   Plasmid

   #12610

   Depositor

   Scot Wolfe

   Article

   Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

   Insert

   Zif268 binding site in pH3U3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 26, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUCIDT-attL1-Human ABeta-attR5

   Plasmid

   #160436

   Purpose

   Entry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombination

   Depositor

   Nicholas Tolwinski

   Article

   Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

   Insert

   Homo sapiens amyloid beta precursor protein (APP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJUMP18-Uac

    Plasmid

    #126959

    Purpose

    Universal Acceptor Plasmid. The fusion sites of the basic part generated are introduced during domestication. sfGFP reporter gene. High copy number oriV #8.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET3a-His-DJ1

    Plasmid

    #51488

    Purpose

    Inducible expression of human His-DJ1 in E.coli

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 31, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. dCas9-CRY2Clust-FUSN-mut

    Plasmid

    #200970

    Purpose

    induction of phase-separated condensate mediated by FUSN-IDR mutant

    Depositor

    Jian Xu

    Article

    Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

    Insert

    FUSNmut
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    15 Tyr-to-Ser substitutions

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-GST-AGO4

    Plasmid

    #24319

    Depositor

    Carl Novina

    Article

    Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

    Insert

    AGO4 (AGO4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pEMI91

    Plasmid

    #74888

    Purpose

    Shuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more information

    Depositor

    Piotr Marszalek

    Article

    Scholl et al Biomacromolecules. 2016 Jun 8.

    Insert

    Titin I27 x 9 (TTN Human)
    

    Use

    Tags

    His-tag and Strep-tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTriEx-mVenus-PA-Rac1-T17N

    Plasmid

    #22017

    Purpose

    Expression of photoactivatable Rac1 (dominant-negative mutant)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
    

    Use

    Tags

    6X His and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Rac1 starts at I4 and contains mutation T17N.

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. p5084

    Plasmid

    #168179

    Purpose

    bacteria reporter

    Depositor

    Michael Hensel

    Article

    Schulte et al Commun Biol. 2021 May 4;4(1):520. doi: 1

    Insert

    PEM7::dsred PmsrA::sfgfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBT3037

    Plasmid

    #122544

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian p62 (sqst-1) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_sqst-1::CemOrange2 (T12G3.1 Synthetic, Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pnhx-2

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET51b-NAD-cpLuc2

    Plasmid

    #135629

    Purpose

    Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V2)

    Depositor

    Kai Johnsson

    Article

    Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

    Insert

    NAD-cpLuc2 sensor
    

    Use

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBEL1092

    Plasmid

    #195635

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOPINO_H3

    Plasmid

    #171927

    Purpose

    Bacterial expression of nanobody VHH_H3, which binds to receptor binding domain of the spike protein of SARS-CoV-2

    Depositor

    Ray Owens

    Article

    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

    Insert

    VHH_H3
    

    Use

    Tags

    His6

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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