30,875 results

1. pmVirD2-GFP

   Plasmid

   #90315

   Purpose

   mVirD2-GFP expression plasmid

   Depositor

   Bjorn Hogberg

   Article

   Bernardinelli et al Nucleic Acids Res. 2017 Oct 13;45(18):e1

   Insert

   A. tumefaciens VirD2 from aa 1 to 204 - GFP
   

   Tags

   6xHis and GFP

   Expression

   Bacterial

   Promoter

   T7/T5 IPTG inducible

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. BASIC_4_Cm

   Plasmid

   #68138

   Purpose

   storage vector for BASIC part: Cm

   Depositor

   Geoffrey Baldwin

   Article

   Storch et al ACS Synth Biol. 2015 Apr 16.

   Insert

   Chloramphenicol
   

   Expression

   Bacterial

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJBL2807

   Plasmid

   #71203

   Purpose

   AD1 STAR Antisense Expression Plasmid

   Depositor

   Julius Lucks

   Article

   Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

   Insert

   AD1 Antisense
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   BBa_J23119

   Available Since

   Jan. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJBL2801

   Plasmid

   #71207

   Purpose

   AD1 Sense Expression Plasmid

   Depositor

   Julius Lucks

   Article

   Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

   Insert

   AD1 Sense
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   BBa_J23119

   Available Since

   Jan. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. EPHA5

   Plasmid

   #25144

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   epha5.653.939 (EPHA5 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 6, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

6. ATX3-77Q

   Plasmid

   #184249

   Purpose

   Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)

   Depositor

   Sandra Macedo Ribeiro

   Article

   Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

   Insert

   Ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track (ATXN3 Human, Synthetic)
   

   Tags

   6x His-Tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Codon optimization for protein expression in BL2…

   Promoter

   T7 promoter

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAW-AlphaLobe

   Plasmid

   #113255

   Purpose

   E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

   Depositor

   Jennifer Doudna

   Article

   Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   S. pyogenes Cas9 alpha-helical lobe
   

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   residues 56–714; See paper for description

   Promoter

   T7

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-Neo-PuroDTK

   Plasmid

   #84036

   Purpose

   PiggyBac cassette contains Neomycin, puromycin and TK genes

   Depositor

   Yuet Wai Kan

   Article

   Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

   Type

   Empty backbone

   Expression

   Bacterial and Mammalian

   Promoter

   CAG promoter

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-4T-1 EGFP-Sumo2 GG

   Plasmid

   #13384

   Depositor

   Mary Dasso

   Article

   Azuma et al J Cell Biol. 2003 Nov 10. 163(3):477-87.

   Insert

   Sumo2 GG (SUMO2 Human)
   

   Tags

   EGFP and GST

   Expression

   Bacterial

   Mutation

   Mature SUMO2GG: diglycine at amino acid positions…

   Available Since

   Nov. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pJB158

    Plasmid

    #202601

    Purpose

    RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.

    Depositor

    Athanasios Typas

    Article

    Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. KLHL11

    Plasmid

    #39019

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KLHL11 (KLHL11 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

12. A3RXB7

    Plasmid

    #177694

    Purpose

    expresses a N-acetyl hexosamine oxidase in E. coli

    Depositor

    Martin Engqvist

    Article

    Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

    Insert

    N-acetylhexosamine oxidase
    

    Tags

    10xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRU1156

    Plasmid

    #14473

    Depositor

    Philip Poole

    Article

    Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

    Insert

    Gfp-mut3.1-GusA
    

    Expression

    Bacterial

    Available Since

    April 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. PXWABS62(E11-TEV)

    Plasmid

    #123157

    Purpose

    For TEV-based leakiness regulation. pSB4A3 carrying ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    ParsR-ABS62, Pecf11

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTWIN1-His6-Ssp-Httex1-23Q

    Plasmid

    #84349

    Purpose

    Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli

    Depositor

    Hilal Lashuel

    Article

    Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

    Insert

    Huntingtin Exon 1 (HTT Human)
    

    Tags

    N-terminal Ssp intein (His-tagged)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmCellFree_KA1 ZIKV E

    Plasmid

    #145341

    Purpose

    Cell-free/mammalian expression vector for Zika virus Envelope protein with N-term eGFP

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV Envelope
    

    Tags

    8xHis and eGFP

    Expression

    Bacterial

    Promoter

    T7- CMV/CAG

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. PARP2A

    Plasmid

    #42450

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PARP2A (PARP2 Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pTn7xTS-sfGFP

    Plasmid

    #117390

    Purpose

    Tn7 tagging vector pTn7xTS with sfGFP expression scaffold

    Depositor

    Karen Guillemin

    Article

    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Ptac

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBbS2k-RFP

    Plasmid

    #35330

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Tet

    Available Since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. HS_NAM-B1

    Plasmid

    #154064

    Purpose

    Level M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosT

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Inserts

    Hygromycin phosphotransferase
    Cre recombinase
    LoxP-flanked GUS and TtNAM-B1
    

    Use

    Cre/Lox and Synthetic Biology; Golden gate

    Expression

    Bacterial and Plant

    Mutation

    The TtNAM-B1 gene sequence was domesticated to re…

    Promoter

    HvHSP17, OsAct, and ZmUbi

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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