32,453 results
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Plasmid#118509Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
ExpressionBacterialAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
GLI-3 bs-2
Plasmid#16420DepositorInsertGLI3 (GLI3 Human)
ExpressionBacterialAvailable SinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI
Plasmid#115214PurposeExpresses PauCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPauCas13
TagsSUMOExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 vph
Plasmid#112893PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertvph
Tags6xHisExpressionBacterialPromoterPblaAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
5T5G (SETD8)
Plasmid#101886PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pExp-Bla
Plasmid#112561PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and Bla (beta-lactamase from the halophile Chromohalobacter sp. fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis-Bla and Strep tagExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
4RCI
Plasmid#64653PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertYTHDF1:JMC02A-E04:C231829 (YTHDF1 Human)
Tags6xHisExpressionBacterialMutationSee commentsPromoterT7Available SinceApril 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-2xMreBN-mCherry-His
Plasmid#133626PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.DepositorInsert2xMreB-mCherry-His
TagsHisExpressionBacterialAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-H391Y
Plasmid#122692PurposeExpress His-tagged PKM2 (H391Y) protein in bacteria.DepositorAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-Venus-pBluescript
Plasmid#166756PurposeFor purification of Venus fluorophoreDepositorInsertVenus
Tags6x Histidine tag, and TEV protease siteExpressionBacterialPromoterT7Available SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pATZ-HA-GFP11
Plasmid#177720PurposeER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to an HA tag and the eleventh β-strand of GFPDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
TagsGFP11 and HAExpressionBacterial and MammalianMutationE342K (ATZ mutant)Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB092a
Plasmid#79217PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (OR1+2); Ppro1Available SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_hGH
Plasmid#182410PurposeBacterial expression of human growth hormoneDepositorAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
WT USP7 TRAF
Plasmid#227226PurposeExpresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationaa 62-205PromoterT7-promoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-VtrA-mCherry
Plasmid#192862PurposepMUT2 based vector for sensing human secondary bile acids in E. coli Nissle 1917DepositorInsertVtrA-CadC fusion protein and cognate promoter inducible by secondary bile acids
ExpressionBacterialAvailable SinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
K980
Plasmid#129762PurposeExpresses Drosophila Kinesin-1 full-length for microtubule gliding assaysDepositorAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7
Plasmid#46306DepositorInsertsp19
DNA-1 site
linker
DNA-2 site
UsePro-sirnaTags6XHis and GSTExpressionBacterialPromoterTacAvailable SinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I
Plasmid#208183Purposemistranslation GFP and mScarlet-I positive controlDepositorInsertsGFPmut2
mScarlet-I
UseReporterExpressionBacterialPromoterPtet+dnaK P1Available SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSUB7
Plasmid#169698PurposeTemplate plasmid for generating C-terminal 6-His epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert6xHis epitope tag adjacent to FRT-flanked aph (KanR) gene
Tags6xHis tagExpressionBacterialAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_LAMA-F98 (for GFP)
Plasmid#130714PurposePlasmid encoding the bacterial expression of LAMA-F98 for GFPDepositorInsertLAMA-F98
TagsHisx10ExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only