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MacroH2A.2-c002

Plasmid

#173089

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROH2A2 (MACROH2A2 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

K167-K372

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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MacroH2A1.2-c002

Plasmid

#173088

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROH2A1 (MACROH2A1 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

Q162-N372, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a(+) 6xHis-TEV-IFIT1

Plasmid

#53557

Purpose

bacterial expression of IFIT1

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT1 (IFIT1 Human)

Tags

6xHIS and TEV

Expression

Bacterial

Promoter

T7

Available Since

May 28, 2014

Availability

Academic Institutions and Nonprofits only
AWP-031

Plasmid

#213967

Purpose

Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for constitutive CRISPR array expression.

Depositor

Alexander Westermann

Article

Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121

Insert

Cas12a (cas12a Segatella bryantii, Synthetic)

Tags

3XFLAG

Expression

Bacterial

Mutation

D875A to inactivate target nuclease activity

Promoter

PcfxA, IPTG inducible

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-iRFP682

Plasmid

#45454

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP682

Expression

Bacterial

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180V, Y193…

Promoter

araBAD

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pUMV19

Plasmid

#67161

Purpose

inducible operation of the mevalonate pathway in E. coli

Depositor

Tomohisa Kuzuyama

Article

Takagi et al J Bacteriol. 2000 Aug;182(15):4153-7.

Insert

the mevalonate pathway genes

Expression

Bacterial

Promoter

lacZ

Available Since

Oct. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pSBLego

Plasmid

#219967

Purpose

The pSBLego vector enables sybodies for the Legobody method with a PelB signal for periplasmic expression and a "SLEHHHHHH" motif for purification and Legobody Fab binding.

Depositor

Markus Seeger

Article

Ackle et al Sci Rep. 2025 Mar 19;15(1):9460. doi: 10

Type

Empty backbone

Tags

SLEHHHHHH and pelB signal sequence

Expression

Bacterial

Promoter

pBAD

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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PET-B-evoSpCas9-NLS-6xHis

Plasmid

#207390

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

Promoter

T7

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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SNX32A-c014

Plasmid

#175486

Purpose

Protein expression in bacterial cells. PX domain, S17-M197

Depositor

Opher Gileadi

Insert

SNX32 (SNX32 Human)

Tags

His6-SUMO

Expression

Bacterial

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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3DAX

Plasmid

#51333

Depositor

Cheryl Arrowsmith

Insert

3DAX

Expression

Bacterial

Mutation

Truncation: See Comments

Promoter

T7

Available Since

Feb. 24, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pCsy4

Plasmid

#44252

Depositor

Adam Arkin, Stanley Qi

Article

Qi et al Nat Biotechnol. 2012 Oct;30(10):1002-6.

Insert

csy4 gene

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLtetO-1

Available Since

April 5, 2013

Availability

Academic Institutions and Nonprofits only

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pFG036

Plasmid

#137996

Purpose

cI repressor expression plasmid

Depositor

Sébastien Rodrigue

Article

Neil et al Commun Biol. 2020 Sep 22;3(1):523. doi:

Insert

cI

Use

Synthetic Biology

Expression

Bacterial

Mutation

Thermosensitive

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pQTEV-CDO1

Plasmid

#31292

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

cysteine dioxygenase, type I (CDO1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pFPV25

Plasmid

#20667

Depositor

Raphael Valdivia

Article

Valdivia et al Mol Microbiol. 1996 Oct . 22(2):367-78.

Type

Empty backbone

Tags

GFP mut3

Expression

Bacterial

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pET28a-MBP-CBX1-eReader

Plasmid

#135089

Purpose

Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

Depositor

Marcey Waters

Article

Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

Insert

CBX1 chromobox protein 1, engineered

Tags

6xhis-tag and MBP

Expression

Bacterial

Mutation

lysine 43 mutated to alanine; aspartic acid 59 mu…

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pBbB19c-GFP

Plasmid

#126139

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBS xenopus Islet1 (DM#219)

Plasmid

#15015

Depositor

Douglas Melton

Article

Kelly et al Dev Dyn. 2000 Aug . 218(4):615-27.

Insert

islet1

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pQE-30-RexYFP

Plasmid

#141070

Purpose

Bacterial expression of genetically encoded fluorescent indicator for measuring NAD+/NADH ratio

Depositor

Vsevolod Belousov

Article

Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

Insert

RexYFP

Tags

6xHis

Expression

Bacterial

Mutation

changed Leucine 169 to Proline, changed Tyrosine …

Promoter

T5

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_RRM2

Plasmid

#157866

Purpose

express His tagged hnRNPA2 RRM2

Depositor

Nicolas Fawzi

Article

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pYTK081

Plasmid

#65188

Purpose

Encodes CEN6/ARS4 as a Type 7 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

CEN6/ARS4

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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