33,526 results
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Plasmid#74076PurposegBT-I TCR a chain cDNA in pGEM-TDepositorInsertTCR alpha clone HSV2.3 (Tcra Mouse)
ExpressionBacterialAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK234
Plasmid#73324PurposeProduces Janthinobacterium lividum violacein biosynthesis proteins VioA, VioB, and VioCDepositorInsertsviolacein biosynthesis protein VioA
violacein biosynthesis protein VioB
violacein biosynthesis protein VioC
ExpressionBacterialPromoterT7 and n/aAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-4A6
Plasmid#73434PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6.DepositorInsertRepressor 4A6 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKS014
Plasmid#65467PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and beta-galactosidaseDepositorInsertNtag-beta galactosidase
UseSynthetic BiologyTagsFLAGExpressionBacterialPromoterpl-TetOAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Num1-PH
Plasmid#71369Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorPublicationInsertNum1-PH
TagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa2563-2692PromotertacAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK367
Plasmid#72406PurposeProduces Acetobacter aceti 1023 Est06DepositorInsertlipase
ExpressionBacterialMutationlacks Met1 and Thr2 in annotated (v1) sequencePromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pR6Kcat-rdxA-rpsL
Plasmid#45471DepositorInsertcat-rdxA-rpsL
ExpressionBacterialAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22-HT/prsA
Plasmid#73624PurposeProduces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tagDepositorInsertribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)
TagsHis6ExpressionBacterialPromoterT7Available sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TOPO CFP
Plasmid#68489PurposeTOPO construct expressing CFP for generation of GMAP-compatible geneDepositorInsertCyan Fluorescent Protein
UseGmapExpressionBacterialPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK225
Plasmid#71124PurposeExpression of Acetobacter aceti 1023 citrate synthase (AaCSH6) in E. coliDepositorInsertcitrate synthase
TagsHis6ExpressionBacterialPromoterT7Available sinceJan. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TSV (linearized in VP2)
Plasmid#70032PurposepUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding regionDepositorInsertTSV full genome, linearized in VP2 gene
UseCloning vectorExpressionBacterialAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-VCPIP (OTU, aa 25-561)
Plasmid#61583PurposeExpresses human VCPIP (OTU domain) in E. coli.DepositorInsertVCPIP (VCPIP1 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-24 and aa 562-1222.PromoterT7Available sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-HsSENP6
Plasmid#71469PurposeEncodes human full length SENP6 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 6 (SENP6 Human)
TagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-STRNDHFR
Plasmid#63978PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneTagsStrep II (STR); TEV cleavableExpressionBacterialPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKS913.CB18.31
Plasmid#69569PurposeTCR beta chain genomic transgenic construct for creating OT-1 miceDepositorPublicationAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT588
Plasmid#68907PurposeNanoLuc expressed constiutively from PBT1311 and GH023 RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
ExpressionBacterialAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT937
Plasmid#68936PurposeRhamnose-inducible Int12 expression vector with rpiL*C51 RBS, pNBU2 backbone, AmpRDepositorInsertInt12
ExpressionBacterialAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
4YWQ
Plasmid#69953PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRC3H1:YTC019-B07:C232791 (RC3H1 Human)
TagsHis and TEV cleavage siteExpressionBacterialPromoterT7-lacOAvailable sinceOct. 7, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pDONR221-ARL8B
Plasmid#67334PurposehArl8b with stop codon in Gateway Entry vectorDepositorAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SAR1
Plasmid#67344PurposehSar1 with stop codon in Gateway Entry vectorDepositorAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only