34,238 results
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Plasmid#103976PurposePlasmid encoding SapphireDepositorInsertSapphire
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (S72A, Y145F,…PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-moxCerulean3
Plasmid#103975PurposePlasmid encoding moxCerulean3DepositorInsertmoxCerulean3
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F64L, Y66W, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mTurquoise2
Plasmid#103974PurposePlasmid encoding mTurquoise2DepositorInsertmTurquoise2
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F64L, Y66W, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mCerulean ME
Plasmid#103971PurposePlasmid encoding mCerulean MEDepositorInsertmCerulean ME
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F64L, S65T, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH514
Plasmid#102667PurposeConstitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
SRpHi3
Plasmid#102543PurposeBacterial expression of sepHluorin fused to the cell penetrating peptide TAT with a GST tag for purificationDepositorInsertTat-sepHluorin
TagsGSTExpressionBacterialPromotertacAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA-RagC(D181N)
Plasmid#99643PurposeBacterial expression of RagA-RagC(D181N)DepositorAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-HRASLS3-1-132
Plasmid#100725PurposeExpression of N-terminal region of human HRASLS3 in E.coliDepositorAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP974-1
Plasmid#99497PurposeTEV::linker::tagRFP::3XFlagDepositorInsertTEV::linker::tagRFP::3XFlag
ExpressionBacterialAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mEos3.1 (mEosFP3.1-A69T)
Plasmid#99221PurposeBacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).DepositorInsertpr-mEos3.1
ExpressionBacterialMutationA69T for primed conversion applicationsPromoterT7Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDB036a
Plasmid#99215PurposeAccessory plasmid for PACE of MjTyrRS variants; negative selectionDepositorInsertPpsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)
ExpressionBacterialMutationamber codons at positions 12 and 203 of T7 RNA po…PromoterPpsp, PproK and PproDAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-ffDronpa-F
Plasmid#101193PurposeA fast switching Dronpa variantDepositorInsertffDronpa-F
Tags6xHis, T7 tag, and Xpress tagExpressionBacterialMutationK45I, V60A, F173VPromoterT7Available sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pXPA-fapO-RgTALsyn
Plasmid#100946PurposeExpression of Tyrosine Ammonia Lyase from constitutive GAP promoterDepositorInsertRgTALsyn
UseSynthetic BiologyExpressionBacterialMutationSilent mutation to remove internal NdeI site on T…PromoterGAP (Constitutive)Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
Keasling-2337
Plasmid#100168PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertlacIq-Ptrc-ADS
ExpressionBacterialPromotertrcAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
Keasling-2187
Plasmid#100167PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertMevT and MBIS operon
ExpressionBacterialPromoterlacUV5Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRIM33 (TRIM33B-c100)
Plasmid#98243PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTRIM33B (TRIM33 Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00628:PHD 889-932, pfam00439:Bromodomain 963-…PromoterT7Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
CFL1 (CFL1A-c002)
Plasmid#98237PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCFL1A (CFL1 Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00241:Cofilin_ADF 27-148PromoterT7Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
HisGb1-STAT1(710-750) + CBP TAZ2 (1764-1855)
Plasmid#99342PurposeBacterial expression of mouse CBP TAZ2 domain by coexpression with HisGB1 tagged STAT1 TADDepositorAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-NCBD + ACTR
Plasmid#99335PurposeBacterial coexpression of CBP NCBD and NCoA3 binding domainsDepositorExpressionBacterialPromoterT7Available sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-beta2(616-951)
Plasmid#100743PurposeBacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice formDepositorAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by