30,234 results

1. pMyCA-mCherry

   Plasmid

   #84272

   Purpose

   mCherry fluorescence protein expression using M. smegmatis

   Depositor

   Young-Hwa Song

   Article

   Magana Vergara et al Protein Sci. 2017 Aug 31. doi: 10.1002/p

   Insert

   mCherry
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Acetamidase

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL-1

   Plasmid

   #180422

   Purpose

   Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a low level RFP promoter.

   Depositor

   Nancy Moran

   Article

   Li et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   Red Chromprotein gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDE43-MCK

   Plasmid

   #49523

   Purpose

   Kanamycin and Chloramphenicol resistant; destination vector engineered to integrate into the mycobacteriophage att-L5 site and designed to be used with the multisite Gateway cloning system

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6CDG (GID4)

   Plasmid

   #119961

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   GID4 (C17orf39 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 124-289

   Promoter

   T7

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pHL 246

   Plasmid

   #53014

   Purpose

   AmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1

   Depositor

   Han Lim

   Article

   Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   fimS (off orientation)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 14, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pET-51b(+)-Caprola_09

   Plasmid

   #194670

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_09 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_09
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

   Plasmid

   #101217

   Purpose

   Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…

   Promoter

   T7

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNRB150

   Plasmid

   #36451

   Depositor

   Douglas Bishop

   Article

   Hong et al J Biol Chem. 2001 Nov 9;276(45):41906-12

   Insert

   DMC1 (DMC1 Budding Yeast)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCoofy31

   Plasmid

   #43993

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Use

   Tags

   His6 and OneStrep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTE-mcs

    Plasmid

    #20320

    Depositor

    Dirk Schnappinger

    Article

    Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

    Insert

    multiple cloning site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETM6-Amp-T7-roGFP

    Plasmid

    #202464

    Purpose

    Expresses redox sensitive GFP (roGFP) with T7 induction

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    reduction-oxidation sensitive green fluorescent protein
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACYCDUET-StrepSUMO-DaPFOR

    Plasmid

    #217819

    Purpose

    Expresses DaPFOR in E. coli

    Depositor

    Pamela Silver

    Article

    Ng et al ACS Chem Biol. 2024 Feb 16;19(2):380-391

    Insert

    pACYCDUET-StrepSUMO-DaPFOR
    

    Use

    Tags

    StrepSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. p4286

    Plasmid

    #198152

    Purpose

    bacterial expression of sseJ::HaloTag fusion

    Depositor

    Michael Hensel

    Insert

    PsseJ sseJ::L16::HaloTag::HA (sseJ Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    HaloTag-HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDH42(CUG/A)

    Plasmid

    #154132

    Purpose

    Expresses engineered (23S-5S phusion) rRNAs operon in bacterial cells

    Depositor

    Alexander Mankin

    Article

    Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

    Insert

    engineered bacterial rRNAs operon under control of pL promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT7C-HSPA1L

    Plasmid

    #177660

    Purpose

    Recombinant protein synthesis of human HSPA1L

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    HSPA1L (HSPA1L Human)
    

    Use

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lapronic/pRSETB

    Plasmid

    #140755

    Purpose

    FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

    Depositor

    Luis Felipe Barros

    Article

    Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

    Insert

    Lapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PIAS2

    Plasmid

    #40743

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PIAS2 (PIAS2 )
    

    Use

    Tags

    6xHis TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pQTEV-PSMD10

    Plasmid

    #31332

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    PSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin) (PSMD10 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBR322-Pdp1_NTD-ZFD_R

    Plasmid

    #198277

    Purpose

    For loading R half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Right-G1397-C_DddA); also contains PVC regulatory genes

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Pdp1_NTD-ZFD_R
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJKR-O-acuR

    Plasmid

    #62569

    Purpose

    Acrylate biosensor. Medium copy plasmid that expresses CFP proportional to acrylate concentration. Compatible with all other pJKR plasmids.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    CFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge