30,875 results

1. pFA6a- mYFP-kanMX6

   Plasmid

   #105147

   Purpose

   Yeast gene targeting

   Depositor

   Thomas Pollard, Jian-Qiu Wu

   Article

   Wu et al Dev Cell. 2003 Nov;5(5):723-34.

   Insert

   mYFP-kanMX6
   

   Tags

   mYFP

   Expression

   Bacterial and Yeast

   Mutation

   A206K

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJL1-YPet

   Plasmid

   #102633

   Purpose

   In vitro expression of YPet from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   YPet
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pShCAST(Cas12k-TnsC)-sgRNA_entry (CJT28)

   Plasmid

   #181789

   Purpose

   Expresses 3-component ShCAST containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ShTnsB, ShTniQ, ShCas12k-ShTnsC
   

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCON1-ProB.mCherry

   Plasmid

   #112520

   Purpose

   ProB driven mCherry expression with synTT

   Depositor

   Olivia Steele-Mortimer

   Article

   Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

   Insert

   ProB mCherry TT
   

   Expression

   Bacterial

   Promoter

   ProB

   Available Since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. POLN_pDEST17

   Plasmid

   #64885

   Purpose

   bacterial expression of POLN for purification

   Depositor

   Richard Wood

   Article

   Takata et al Nucleic Acids Res. 2010 Jun;38(10):3233-

   Insert

   POLN (POLN Human)
   

   Tags

   Flag and His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. TOPO Cre-2A-GFP

   Plasmid

   #68450

   Purpose

   TOPO construct expressing Cre-2A-GFP for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Cre-2A-GFP
   

   Use

   Gmap

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. INCENP

   Plasmid

   #39185

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   INCENP (INCENP Human)
   

   Tags

   GST-Tev

   Expression

   Bacterial

   Mutation

   Complex with AURKBA-c004

   Available Since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

8. GST-CHMP4A (pGEX2T(TEV))

   Plasmid

   #80630

   Purpose

   Bacterial Expression Vector; TEV cleavable GST-tag Internal ID: WISP06-197

   Depositor

   Wesley Sundquist

   Article

   McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

   Insert

   CHMP4A (CHMP4A Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Residues 1-222

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-KG-mTmod3-WT

   Plasmid

   #70753

   Purpose

   mouse Tropomodulin3, WT for bacterial expression

   Depositor

   Weiping Han

   Article

   Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

   Insert

   Tropomodulin3 (Tmod3 Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSUMO-β′-COPI-WD40

    Plasmid

    #180753

    Purpose

    Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40 (residues 1-301).

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    β′-COPI-WD40 (SEC27 Budding Yeast)
    

    Tags

    N-terminal strep tag, His-tag, and SUMO tag

    Expression

    Bacterial

    Mutation

    Encodes residues 1-301; deleted 302-889; mutated …

    Promoter

    T7

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-45b-HP1a-mCherry

    Plasmid

    #185012

    Purpose

    Bacterial Expression of 6xHis CBX5 (HP1a) mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    CBX5 (HP1a) (Cbx5 Mouse)
    

    Expression

    Bacterial

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. LaM8-AK74

    Plasmid

    #159594

    Purpose

    Opto-Nanobody

    Depositor

    Jose Avalos, Jared Toettcher

    Article

    Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

    Insert

    LaM8 with AsLOV2 at AK74
    

    Expression

    Bacterial

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. UBC32

    Plasmid

    #25213

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ubc32.001.158 (UBE2K Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 12, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pET_BGL2

    Plasmid

    #101911

    Purpose

    Expresses a β-glucosidase gene from Thermobaculum terrenum in E. coli under the control of the pET system.

    Depositor

    Steve Shih

    Article

    Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944

    Insert

    β-glucosidase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7+LacO

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMU-4

    Plasmid

    #172353

    Purpose

    CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA

    Depositor

    Eriko Takano

    Article

    Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

    Insert

    D4-TheoR-cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    D4

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. OmpA-mNG-mCh

    Plasmid

    #124216

    Purpose

    Periplasmic OM bound mNeongreen mCherry tandem, ~16 % energy transfer. pNM004 - pTHV037-OmpA-SA1-177-(SA-1)-LEDPPAEL-mNG-mCh

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al MBio. 2017 Sep 12;8(5). pii: mBio.01089-

    Insert

    OmpA-mNG-mCh
    

    Expression

    Bacterial

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEB1-T-Sapphire

    Plasmid

    #103977

    Purpose

    Plasmid encoding T-Sapphire

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    T-Sapphire
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (Q69M, C70V, …

    Promoter

    proC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET24b-BR1.0

    Plasmid

    #199435

    Purpose

    expresses BAmRed1.0 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMRed
    

    Tags

    his tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXD69m1-TetW

    Plasmid

    #191246

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid with tetracycline resistance in E. lenta

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pH3U3-Zif268

    Plasmid

    #12610

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

    Insert

    Zif268 binding site in pH3U3
    

    Expression

    Bacterial

    Available Since

    Sept. 26, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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