30,234 results

1. pFAB808

   Plasmid

   #47853

   Purpose

   BIOFAB reporter plasmid for measuring rrnB T1 termination efficiency

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   rrnB T1 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLTetO8

   Available since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDuet1-His-TEV-OTC_Ub

   Plasmid

   #172134

   Purpose

   Scaffold design for studying ubiquitin interacting proteins by cryo-EM.

   Depositor

   Shang-Te Danny Hsu

   Article

   Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

   Insert

   E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-FLAG-SpCas9-HF1

   Plasmid

   #126770

   Purpose

   Expression of increased fidelity SpCas9-HF1 in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   T7

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. SRpHi4

   Plasmid

   #102544

   Purpose

   Bacterial expression of EYFP fused to Epidermal Growth Factor with a GST tag for purification

   Depositor

   Stefan Hell

   Article

   Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

   Insert

   EYFP-EGF
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMU102

   Plasmid

   #61805

   Purpose

   Cloning and expression vector for Clostridium thermocellum

   Depositor

   Lee Lynd

   Article

   Olson et al J Biol Eng. 2012 May 11;6(1):5. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. RNF168

   Plasmid

   #25298

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   rnf168.0001.0113 (RNF168 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 16, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pET28a_Pro-IL-36γ

   Plasmid

   #214345

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL36G (IL36G Human)
   

   Use

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. EcAvs4

   Plasmid

   #188794

   Purpose

   Avs4 from Escherichia coli NCTC11132

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

   Insert

   EcAvs4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-4T-1-RIPK3

   Plasmid

   #78827

   Purpose

   To purify GST-tagged recombinant RIPK3 protein

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK3 (RIPK3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD_Ub11TCA

    Plasmid

    #174517

    Purpose

    Recoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitin

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Insert

    Ubiquitin
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Recoded with TCA replacing codon 11 of wt ubiquit…

    Promoter

    araBAD

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMBP-LbCas12a D832A

    Plasmid

    #113517

    Purpose

    Expresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nuclease

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    LbCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    D832A

    Promoter

    T7

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas_5+7_gfp

    Plasmid

    #60595

    Purpose

    encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Cas_5+7_gfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas_2+5

    Plasmid

    #60594

    Purpose

    encodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Cas_2+5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFVTd5

    Plasmid

    #167520

    Purpose

    Broad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, tdKatushka2, Gentamicin Resistance Marker

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    VanRAM-tdKatushka2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PVanCC

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSV272-PfMBP-HypDAE

    Plasmid

    #110296

    Purpose

    Expresses t-4-hydroxy-L-proline dehydratase activating enzyme in E. coli BL21(DE3)

    Depositor

    Emily Balskus

    Article

    Levin et al Science. 2017 Feb 10;355(6325). pii: 355

    Insert

    t-4-Hydroxy-L-proline Dehydratase Activating Enzyme
    

    Use

    Tags

    His6 and Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pColdII-SIRPT1-Xenopus (Silurana) tropicalis

    Plasmid

    #75355

    Purpose

    Nt His-tag Xenopus (Silurana) tropicalis Sacsin 87-1376

    Depositor

    Kalle Gehring

    Insert

    Sacsin Xenopus (Silurana) tropicalis 87-1376
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEBG-NHERF2

    Plasmid

    #28292

    Depositor

    Maria-Magdalena Georgescu

    Article

    Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

    Insert

    NHERF2 (NHERF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pMC051-HisMS2_PLP_pac_LC

    Plasmid

    #128234

    Purpose

    Lower Copy expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

    Depositor

    Paul Freemont

    Article

    de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

    Inserts

    Maturation Protein
    Coat Protein Dimer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 15, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pET30b_T7Sav

    Plasmid

    #138588

    Purpose

    T7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori

    Depositor

    Markus Jeschek

    Article

    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

    Insert

    T7-tagged streptavidin variant with native C-terminus
    

    Use

    Synthetic Biology

    Tags

    T7-tag

    Expression

    Bacterial

    Mutation

    Promoter

    PT7

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGGA_RT_pLexA

    Plasmid

    #136961

    Purpose

    Goldengate/Greengate module for generation of beta-estradiole inducible genes.

    Depositor

    Klaus Theres

    Insert

    pLexA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge