30,875 results

1. pC029 - Lw2Cas13a from Leptotrichia wadei F0279

   Plasmid

   #91919

   Purpose

   Expresses Lw2Cas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   Lw2Cas13a and guide expression cassette
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. DT035A

   Plasmid

   #159397

   Purpose

   Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tac promoter

   Depositor

   Sheref Mansy

   Article

   Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

   Insert

   pr.tac-sfGFP
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SEPX1

   Plasmid

   #39048

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   SEPX1 (MSRB1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   SeCys95-S

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pMGA-ptet-sfGFP

   Plasmid

   #139935

   Purpose

   aTc-inducible sfGFP expression on M. magneticum/E. coli shuttle vector

   Depositor

   Christopher Voigt

   Article

   Furubayashi et al Adv. Funct. Mater. 2021, 31, 2004813

   Insert

   TetR-Ptet-sfGFP
   

   Expression

   Bacterial

   Available Since

   July 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. RhoC FLARE.sc mCer, mVenus - CA

   Plasmid

   #65424

   Purpose

   RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L

   Depositor

   Klaus Hahn

   Article

   Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

   Insert

   RBD, mCerulean, mVenus, RhoC
   

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   T153M in mVenus, Q63L in RhoC

   Available Since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

6. RhoC FLARE.sc mCer, mVenus - DN

   Plasmid

   #65423

   Purpose

   RhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N

   Depositor

   Klaus Hahn

   Article

   Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

   Insert

   RBD, mCerulean, mVenus, RhoC
   

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   T153M in mVenus, T19N in RhoC

   Available Since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pMCSGC-GB1

   Plasmid

   #186786

   Purpose

   E. coli expression vector containing N-terminal 6His-GB1-TEV fusion.

   Depositor

   Alexei Savchenko

   Article

   Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMTTH+mEGFP-H1.4

   Plasmid

   #157799

   Purpose

   Expression of mEGFP and human linker histone H1.4 fusion protein

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   mEGFP-H1.4 (H1-4 Human)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Promoter

   pTAC

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDB038a

   Plasmid

   #99211

   Purpose

   Accessory plasmid for PACE of PylRS variants

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Ppsp [SD8] gIII(P29*,Y184*), luxAB; PproK pylT
   

   Expression

   Bacterial

   Mutation

   amber codons at positions 29 and 184 of gIII

   Promoter

   Ppsp and PproK

   Available Since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SIRT5.1

    Plasmid

    #13738

    Depositor

    John Denu

    Article

    Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

    Insert

    SIRT5 (SIRT5 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pLyGo-Ec-7

    Plasmid

    #163135

    Purpose

    pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Nanobody, C-IgAP autotransporter

    Expression

    Bacterial

    Promoter

    P(rhaB)

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYH74

    Plasmid

    #217382

    Purpose

    pTrc99A-mNeonGreen-mcdB for bacterial expression

    Depositor

    Anthony Vecchiarelli

    Article

    Hoang et al Nat Commun. 2024 Apr 15;15(1):3222. doi:

    Insert

    mcdB
    

    Tags

    NeonGreen

    Expression

    Bacterial

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBSV2G_PflaB-msfYFPBb

    Plasmid

    #118233

    Purpose

    Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfYFP

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    flagellin promoter driven msfYFP
    

    Expression

    Bacterial

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSB1C3 I0500 LuxI

    Plasmid

    #107965

    Purpose

    Arabinose-inducible 3OC6HSL sender plasmid

    Depositor

    Jim Haseloff

    Article

    Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

    Insert

    luxI
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mOrange pHis-//

    Plasmid

    #53304

    Purpose

    Empty vector to express N-terminal His-mOrange-tagged fusion protein.

    Depositor

    John Lis

    Article

    Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

    Type

    Empty backbone

    Tags

    His and mOrange

    Expression

    Bacterial

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOGG024

    Plasmid

    #113991

    Purpose

    Level 1 golden gate cloning vector for medium copy number free-living expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOPINTRX

    Plasmid

    #41116

    Depositor

    Ray Owens

    Article

    Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

    Type

    Empty backbone

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    March 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBS hBAF57

    Plasmid

    #17877

    Depositor

    Jerry Crabtree

    Article

    Chi et al Nature. 2002 Jul 11. 418(6894):195-9.

    Insert

    BAF57 (SMARCE1 Human)
    

    Expression

    Bacterial

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pET28a-B4E

    Plasmid

    #162067

    Purpose

    For expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4E

    Depositor

    Karen Polizzi

    Article

    Moya-Ramirez et al Nucleic Acids Res. 2020 Dec 16;48(22):e1

    Insert

    ampR-eIF4E fusion protein
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    eIF4E incorporating the K119A mutation, which is …

    Promoter

    T7

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIKM1

    Plasmid

    #51054

    Purpose

    Kanamycin and erethromycin resistance

    Depositor

    Lee Lynd, Juergen Wiegel

    Article

    Tyurin et al Appl Environ Microbiol. 2004 Feb;70(2):8

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 19, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge