31,828 results
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Plasmid#225290PurposeE. coli-Campylobacter shuttle vector; KanRDepositorInsertReplication elements from pCC388 for pJBM3
ExpressionBacterialAvailable SinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbS5c-Maqu_3494
Plasmid#86205PurposeExpresses efflux pump in E. coli to provide resistance to biofuel (low copy)DepositorInsertEfflux pump
ExpressionBacterialPromoterATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…Available SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZE31ms2
Plasmid#25860DepositorInsertms2 binding site (x2)
ExpressionBacterialMutationnoneAvailable SinceNov. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET21-GFP-AURKA
Plasmid#99875PurposeExpression of GFP-AURKA for protein purification in bacteriaDepositorAvailable SinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYTK089
Plasmid#65196PurposeEncodes AmpR-ColE1 as a Type 8a part to be used in the Dueber YTK systemDepositorInsertAmpR-ColE1
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher EQ
Plasmid#35045DepositorInsertSpyCatcher EQ
TagsHis6ExpressionBacterialMutationDeletion of C-terminal till I113, and point mutat…Available SinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
RAB25
Plasmid#26163PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCRII-1.1-1.10a
Plasmid#69589PurposeOT-II TCR a chain cDNA in pCRIIDepositorInsertTCR alpha clone 1.1 (Tcra Mouse)
ExpressionBacterialAvailable SinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJN105-Hygro-GFP
Plasmid#41883DepositorInsertPtac::GFP-mut-2
UseBacterial broad host range vectorExpressionBacterialMutationGFP-mut-2 is GFP with Ser65Ala, Val68Leu, and Ser…PromoterPtacAvailable SinceJune 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
RS35075_pMQ30_KO_Kan
Plasmid#185390PurposeNon-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11DepositorInsertsRR42_RS35075 upstream flanking homology region
RR42_RS35075 downstream flanking homology region
nptII kanamycin resistance marker
ExpressionBacterialAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAV10.KH
Plasmid#63208PurposepCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTEV5_ RpMatB_1
Plasmid#78205PurposeExpression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensorDepositorInsertmatB (His6 C106A R286C Q457C K488A)
TagsHis6ExpressionBacterialMutationC106A R286C Q457C K488APromoterT7Available SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
PclpB riboswitch GFP pZa
Plasmid#170078PurposeGFP expression under the control of E. coli clpB promoter engineered with riboregulatorsDepositorInsertGreen fluorescent protein
UseSynthetic BiologyExpressionBacterialPromoterPclpBAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSecUAG_UTu1D
Plasmid#233867PurposeExpresses machinery necessary for selenocysteine incorporation at UAG codons in BL21-derived cells, spectinomycin resistantDepositorInsertsallo-tRNA UTu1D
thioredoxin
Selenophosphate synthase
Selenocysteine synthase
ExpressionBacterialMutationCysteine 32 switched to Selenocysteine (UAG) and …PromoterEM7, araBAD, and native As SelDAvailable SinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB117
Plasmid#228481Purpose"Empty" C-terminal HRV-3C cleavable GST tag vector for rapid cloning/DNA Assembly via NheI restriction enzyme site (Bacterial Expression)DepositorTypeEmpty backboneTagsGST (glutathione S-transferase)ExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET41c-PhTET2
Plasmid#182428PurposeExpresses P. horikoshii tetrahedral aminopeptidase 2 (TET2) in E. coliDepositorInsertTetrahedral aminopeptidase TET2 from P. hirokoshii
ExpressionBacterialAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-30
Plasmid#172754PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-30
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
PERK 4: PERKKD-pGEX4T-1
Plasmid#21817DepositorInsertPERK (537-1114) (Eif2ak3 Mouse)
TagsGST and Myc (9E10)ExpressionBacterialMutationPERK Kinase Domain. Amino acids 537-1114.Available SinceMarch 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-A4DARPin
Plasmid#198288PurposeProduces PVCs retargeted with anti-lysozyme DARPin A4DepositorInsertPVCpnf_pvc13-A4DARPin
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
EcMBP165-cpGFP.PPYF.pRSET
Plasmid#33372Purposea single-wavelength maltose sensor constructed from E. coli MalE and cpGFPDepositorInsertMBP based Maltose Biosensor
Tags6xHISExpressionBacterialMutationcpGFP146 variant from GCaMP2 inserted between AA1…PromoterT7Available SinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only