30,235 results

1. pHAGE2-TetOminiCMV-Sox2

   Plasmid

   #136612

   Purpose

   Dox-inducible lentiviral vector expressing mouse Sox2

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Sox2 (Sox2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetO-miniCMV (dox-inducible)

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28b-miniR-DBCO

   Plasmid

   #181984

   Purpose

   Used to label proteins with the MiniE genetically encoded tag

   Depositor

   Kimberly Beatty

   Article

   Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

   Insert

   MiniR-DBCO
   

   Use

   Tags

   DBCO-Tag, His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBbB11a-GFP

   Plasmid

   #126114

   Purpose

   Constitutive expression vector for Escherichia coli. BglBrick compatible.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

   Insert

   GFPuv
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23150

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-2T-GST-RNMT

   Plasmid

   #112709

   Purpose

   Expresses N-terminally GST-tagged human RNMT in bacterial cells

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   RNMT (RNMT Human)
   

   Use

   Tags

   GST and thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEB1-mClover3

   Plasmid

   #103994

   Purpose

   Plasmid encoding mClover3

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mClover3
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (S30R, Y39N, …

   Promoter

   proC

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

   Plasmid

   #128404

   Purpose

   Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Exotoxin A (toxA P. aeruginosa)
   

   Use

   Tags

   6xHis tag and DsbA signal sequence for periplasmi…

   Expression

   Bacterial

   Mutation

   242DNNNS246 and 384DQNRT388 engineered glycosylat…

   Promoter

   trc

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR201-Eps8L2

   Plasmid

   #196526

   Purpose

   Entry vector for Gateway with Eps8L2

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Eps8L2-FL (EPS8L2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   CMV promoter; T7 promoter

   Available since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKW08-Lx-Int

   Plasmid

   #25015

   Depositor

   Brian Robertson

   Article

   Williams et al Plasmid. 2010 Sep;64(2):69-73. doi: 10.1

   Insert

   pKW08-Lx-Int
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-Tight-Puro_Nkx6.2

   Plasmid

   #64846

   Purpose

   A lentiviral vector for the expression of mouse Nkx6.2 under doxycyline control

   Depositor

   Paul Tesar

   Article

   Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

   Insert

   Nkx6.2 (Nkx6-2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET22B_PcrA_2Bchimera

    Plasmid

    #102998

    Purpose

    Express a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA

    Depositor

    Martin Webb

    Insert

    PcrA_2Bchimera
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Bacilllus stearothermophilus PcrA in which the 2B…

    Promoter

    T7

    Available since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. CuGFP-ATG8(416)

    Plasmid

    #49423

    Purpose

    Expresses ATG8 fused at the N terminus to GFP under the control of the CUP1 promoter.

    Depositor

    Daniel Klionsky

    Article

    Wang et al J Biol Chem. 2012 Nov 2;287(45):37964-72

    Insert

    autophagy-related 8 (ATG8 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pET28a-SUMO-SpyTag003

    Plasmid

    #216282

    Purpose

    Expresses SUMO with a C-terminal SpyTag003 in bacterial cells

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SUMO-SpyTag003
    

    Use

    Tags

    His6 and SpyTag003

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNIC-HIS6-THRX-TEV-GG

    Plasmid

    #215796

    Purpose

    Cloning of target gene with N terminal His6 Thioredoxin tag and TEV cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. DNMT3A

    Plasmid

    #25328

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DNMT3A_11 (DNMT3A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pETNKI-his-SUMO-RNF168ring (1-189)

    Plasmid

    #63570

    Purpose

    Expression of human RNF168 ring domain (residues 1-189) tagged with His-SUMO

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    6xHis and SUMO3

    Expression

    Bacterial

    Mutation

    Ring domain only (residues 1-189)

    Promoter

    T7

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pFP.R272

    Plasmid

    #138221

    Purpose

    Expresses the split mCherry reporter interrupted by the SspGyrB artificially mini-intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(AGG).SspGyrB.(SAK)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX5X.1 PINK1 KD

    Plasmid

    #13322

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 kinase domain (PINK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Amino acids 112-496; Kinase dead: K219D/D362A/D3…

    Promoter

    Available since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

18. mTagRFP-pBAD

    Plasmid

    #54539

    Purpose

    Localization: Protein Expression Vector , Excitation: 555, Emission: 584

    Depositor

    Dmitriy Chudakov, Dorus Gadella, Michael Davidson

    Article

    Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

    Type

    Empty backbone

    Use

    Tags

    6xHis and mTagRFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pJJDuet30

    Plasmid

    #11962

    Depositor

    Hideo Iwai

    Article

    Zuger et al Nat Biotechnol. 2005 Jun . 23(6):736-40.

    Insert

    Ssp DnaE-N
    

    Use

    Tags

    GB1

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmCellFree_KA1_ZIKV NS5

    Plasmid

    #145348

    Purpose

    Cell-free/ mammalian expression vector for Zika virus NS5 protein with N-term eGFP

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS5
    

    Use

    Tags

    8xHis and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7- CMV/CAG

    Available since

    March 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge