30,877 results

1. pDule2-IBBN (G2)

   Plasmid

   #85501

   Purpose

   Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

   Insert

   IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Expression

   Bacterial

   Mutation

   Y32G L65E F108W Q109M D158S R162K

   Promoter

   lpp (constitutive)

   Available Since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSB1C3-H3

   Plasmid

   #109381

   Purpose

   Arabinose inducible VioB-mCherry expression (weaker RBS). Used to test burden response in E. coli

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

   Insert

   pARABAD-VioB-mCherry
   

   Expression

   Bacterial

   Available Since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRE-Tn5-131

   Plasmid

   #118517

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mTurquoise2
   

   Use

   Minitn5 delivery vector

   Expression

   Bacterial

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. phage UbiC SNAP-KDEL

   Plasmid

   #104982

   Purpose

   Lentiviral vector expressing SNAP-KDEL for ER labeling

   Depositor

   Jeffrey Chao

   Article

   Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

   Insert

   SNAP-KDEL
   

   Use

   Lentiviral

   Tags

   Secretion signal

   Expression

   Bacterial and Mammalian

   Promoter

   UbiC

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET29b-clbBpks

   Plasmid

   #114154

   Purpose

   Expresses the PKS module of ClbB

   Depositor

   Emily Balskus

   Article

   Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

   Insert

   clbB
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Only contains the PKS module of ClbB

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSPB845

   Plasmid

   #128296

   Purpose

   asymmetric plasmid partitioning E. coli - regulatory plasmid

   Depositor

   Matthew Bennett

   Article

   Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

   Insert

   PLlacO1 - lp-cfp-parB, BBa_J23110 - lacI
   

   Expression

   Bacterial

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. SBC015866

   Plasmid

   #226280

   Purpose

   Expresses BsSfp, MsCAD, and SrCAR from trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.

   Depositor

   Rainer Breitling

   Article

   Hanko et al Microb Cell Fact. 2023 Nov 18;22(1):238.

   Inserts

   4'-phosphopantetheinyl transferase Sfp
   Cinnamyl alcohol dehydrogenase
   Carboxylic acid reductase
   

   Expression

   Bacterial

   Available Since

   Nov. 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pH6HTC_IGF2BP1-Halo

   Plasmid

   #175300

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   IGF2BP1 (IGF2BP1 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDM4-GLY

   Plasmid

   #49808

   Purpose

   Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.

   Depositor

   Mattheos Koffas

   Article

   Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

   Inserts

   phosphoglycerate kinase
   glyceraldehyde-3-phosphate dehydrogenase
   pyruvate dehydrogenase subunit E1
   dihydrolipoamide acetyltransferase
   dihydrolipoamide dehydrogenase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGV3340

    Plasmid

    #170278

    Purpose

    Expression of mutant HIV-2 RT p51 reverse transcriptase

    Depositor

    Gregory Van Duyne

    Article

    Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

    Insert

    HIV-2 RT p51 (mutant)
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Promoter

    T7-lac

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-pelB-LaG-41

    Plasmid

    #172757

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-41
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. KG#203

    Plasmid

    #110880

    Purpose

    Expresses the C. elegans pde-4 (isoform d) cDNA pan-neuronally

    Depositor

    Kenneth Miller

    Article

    Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1

    Inserts

    rab-3 promoter
    pde-4d (isoform d) cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Expression

    Bacterial

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. 6NM4

    Plasmid

    #162257

    Purpose

    Bacterial Expression

    Depositor

    Cheryl Arrowsmith

    Insert

    PRDM9 (PRDM9 Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pQO18-TEV

    Plasmid

    #27399

    Depositor

    Jonathan Javitch

    Article

    Zhao et al Nature. 2010 May 13. 465(7295):188-93.

    Insert

    leuT
    

    Tags

    HisX10 and TEV

    Expression

    Bacterial

    Available Since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPBO.ACT003

    Plasmid

    #182713

    Purpose

    aTc inducible dCasRx-IF3

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    dCasRx
    

    Use

    CRISPR

    Tags

    3x(GGGS)-Escherichia Coli Initiation Factor 3

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pTet

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET51b-NAD-cpLuc2

    Plasmid

    #135629

    Purpose

    Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V2)

    Depositor

    Kai Johnsson

    Article

    Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

    Insert

    NAD-cpLuc2 sensor
    

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNIC-CH10-GG

    Plasmid

    #215807

    Purpose

    Cloning of target gene with C terminal non cleavable His10 tag

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pQStrep2-ARPC3

    Plasmid

    #31580

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    Actin related protein 2/3 complex, subunit 3 (ARPC3 Human)
    

    Tags

    His and StrepII-tag

    Expression

    Bacterial

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEN12A-P1

    Plasmid

    #27366

    Depositor

    Dirk Schnappinger

    Article

    Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

    Insert

    Pmyc1tetO
    

    Use

    Gateway donor vector

    Expression

    Bacterial

    Mutation

    N/A

    Available Since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET30b_T7Sav

    Plasmid

    #138588

    Purpose

    T7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori

    Depositor

    Markus Jeschek

    Article

    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

    Insert

    T7-tagged streptavidin variant with native C-terminus
    

    Use

    Synthetic Biology

    Tags

    T7-tag

    Expression

    Bacterial

    Promoter

    PT7

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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