30,235 results

1. pMRE-Tn5-154

   Plasmid

   #118536

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mOrange2
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pXDC61

   Plasmid

   #21841

   Depositor

   Howard Shuman

   Article

   Charpentier et al PLoS Pathog. 2009 Jul . 5(7):e1000501.

   Insert

   blaM
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 2, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCD8-GFP in pBS

   Plasmid

   #17745

   Depositor

   Liqun Luo

   Article

   Lee et al Neuron. 1999 Mar . 22(3):451-61.

   Insert

   mouse CD8-GFP (Cd8a Mouse)
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 28, 2008

   Availability

   Academic Institutions and Nonprofits only

4. Top7 V48V

   Plasmid

   #12393

   Depositor

   David Baker

   Article

   Kuhlman et al Science. 2003 Nov 21. 302(5649):1364-8.

   Insert

   Top7 V48V
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 13, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)

   Plasmid

   #181788

   Purpose

   Expresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pWzyE-distrepII

   Plasmid

   #196667

   Purpose

   Expression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   WzyE
   

   Use

   Tags

   TEV-cleavable di-strep tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. UMG-SP-1_pET29b

   Plasmid

   #215433

   Purpose

   Expression construct for UMG-SP-1 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   UMG-SP-1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. UMG-SP-2_pET29b

   Plasmid

   #215434

   Purpose

   Expression construct for UMG-SP-2 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   UMG-SP-2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mouse ptc probe M2-3

   Plasmid

   #58701

   Purpose

   makes mouse ptc probe for in situ hybridization

   Depositor

   Matthew Scott

   Insert

   ptc (Ptch1 Mouse)
   

   Use

   In situ probe

   Tags

   Expression

   Bacterial

   Mutation

   only EcoRI fragment (5' end)

   Promoter

   T7

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

10. PTPRK

    Plasmid

    #38968

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PTPRK (PTPRK Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

11. RanBP2-RB3-4-pET23a

    Plasmid

    #85769

    Purpose

    bacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tag

    Depositor

    Frauke Melchior

    Article

    Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

    Insert

    RanBP2 fragment (RANBP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    fragment (amino acids 2304-3062)

    Promoter

    T7

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET14b_tev_PDZ1

    Plasmid

    #59739

    Purpose

    expresses PDZ1 domain of human ZO-1 in bacteria

    Depositor

    Alan Fanning

    Insert

    zonula occludens-1 (TJP1 Human)
    

    Use

    Tags

    HIS tag and thrombin clvg

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pBAD/HisB-PhoCl

    Plasmid

    #87688

    Purpose

    Express PhoCl in E. coli

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PhoCl
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p2B-57

    Plasmid

    #177927

    Purpose

    Expresses yeGFP under control of doxycycline-responsive TET3 promoter (contains three rtTA binding sites)

    Depositor

    Vadim Gladyshev

    Article

    Barre et al (unpublished)

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TET3 (contains three rtTA binding sites)

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-3a_mb2m

    Plasmid

    #211368

    Purpose

    Expresses murine b2m

    Depositor

    Adnane Achour

    Insert

    b2m (B2m Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pQCasTns(Vch)-entry(BsaI)

    Plasmid

    #190273

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

    Insert

    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(BsaI)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    no

    Promoter

    Available since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTG131

    Plasmid

    #198640

    Purpose

    Thermosensitive pSC101-based plasmid expressing a sgRNA under the control of the J23119 promoter and Cas9 under the control of the DAPG-inducible PhlF promoter

    Depositor

    David Bikard

    Article

    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pPhlF

    Available since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGI3EM09

    Plasmid

    #135482

    Purpose

    Agrobacterium T-DNA with SpunHsp70pr driving hph-GFP

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    SpunHsp70pr hph-GFP ADH1t
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunHsp70pr

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTU1-A-pdh_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid

    #107576

    Purpose

    B. megaterium DSM319 pdh promoter, GFP+ with malachite green mRNA aptamer in Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

    Depositor

    Paul Freemont

    Article

    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

    Insert

    GFP+
    

    Use

    Synthetic Biology

    Tags

    B. megaterium DSM319 xylA leader sequence (MTSSKI…

    Expression

    Bacterial

    Mutation

    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

    Promoter

    pdh promoter Bacillus megaterium DSM319

    Available since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJBL7025

    Plasmid

    #128810

    Purpose

    Fluoride riboswitch-regulated catechol 2,3-dioxygenase

    Depositor

    Julius Lucks

    Article

    Thavarajah et al ACS Synth Biol. 2019 Dec 12. doi: 10.102

    Insert

    Fluoride riboswitch-regulated catechol 2,3-dioxygenase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge