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p15A-OptoCre-cat-P-R

Plasmid

#189853

Purpose

Cre-inducible activation of cat for chloramphenicol resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P-loxP-TT-loxP-R-cat

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

P (T7 phage gene 10)

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD14

Plasmid

#208266

Purpose

Production of syn-pimara-7,15-diene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL4

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_DNAJB1_DNAJ

Plasmid

#109918

Purpose

Protein expression and purification of DNAJB1_DNAJ

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DNAJB1_DNAJ

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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GST-FOXO6

Plasmid

#24381

Depositor

Anne Brunet

Article

Greer et al J Biol Chem. 2007 Oct 12. 282(41):30107-

Insert

FOXO6 (FOXO6 Mouse)

Tags

GST

Expression

Bacterial

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only
pNtCp-rbcL-PHLS-GPPS-aadA-accD

Plasmid

#52471

Purpose

Inserts the beta-phellandrene synthase gene, the geranyl diphosphate synthase gene, and aadA resistance in the rbcL-accD intergenic region in Nicociana tabacum

Depositor

Anastasios Melis

Insert

rbcL-PHLS-GPPS-aadA-accD

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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GST-CHMP4A (pGEX2T(TEV))

Plasmid

#80630

Purpose

Bacterial Expression Vector; TEV cleavable GST-tag Internal ID: WISP06-197

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4A (CHMP4A Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 1-222

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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ALC1-c005

Plasmid

#173092

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

CHD1L (CHD1L Human)

Tags

mCerulean

Expression

Bacterial

Mutation

L613-K879, codon optimized

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pUltra-FPheKRS

Plasmid

#136631

Purpose

Genetic Incorporation 4-fluorophenyl carbamate lysine (FPheK) into proteins

Depositor

Han Xiao

Article

Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

Insert

FPheKRS

Expression

Bacterial

Promoter

Tac1 promoter

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_gpN:SnoopTag

Plasmid

#225195

Purpose

Used for adding C-terminal SnoopTag to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SnoopTag

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19-HDRT-CD3z-truncCARgsg(anti-CD19)

Plasmid

#215759

Purpose

HDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2 (enhanced expression by GSG-2A linker)

Depositor

Dimitrios Wagner

Article

Kath et al Blood. 2024 Mar 17:blood.2023020973. doi

Insert

CD247-truncCD19CAR-GSG (CD19, CD247 Human)

Tags

IgG1 CH3

Expression

Bacterial

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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EV-A71 2A protease; aliases: Enterovirus EV71 2A protease, 6xHis-SUMO-TEV tag, self cleaving

Plasmid

#204810

Purpose

Bacterial expresison of Enterovirus A71 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

A71 2A protease

Expression

Bacterial

Available Since

Dec. 18, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pHH0103_GRB2_SH3

Plasmid

#110015

Purpose

Protein expression and purification of GRB2_SH3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GRB2_SH3 (GRB2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAN3945

Plasmid

#74699

Purpose

Circuit plasmid: 0x41

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

Circuit 0x41

Expression

Bacterial

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-lldR

Plasmid

#74095

Purpose

L-lactate repressor (LldR) from E. coli MG1655

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

lldR

Use

Synthetic Biology

Expression

Bacterial

Promoter

None

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET26_MraYaa

Plasmid

#100166

Purpose

recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Chung et al Science. 2013 Aug 30;341(6149):1012-1016

Insert

mraY (phospho-MurNAc-pentapeptide translocase)

Tags

MBP-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-I48V_AnaCNJKFGW_Bba-B0015

Plasmid

#202023

Purpose

Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coli

Depositor

Mikhail Shapiro

Article

Hurt et al bioRxiv 2024.03.30.587094

Insert

Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant)

Expression

Bacterial

Mutation

GvpA T6A-I48V

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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HS-aPKC-CAAX-mKate2

Plasmid

#105950

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

aPKC-CAAX

Tags

mKate2

Expression

Bacterial

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-mCherryGGBsMTS

Plasmid

#133625

Purpose

Expression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.

Depositor

Petra Schwille

Article

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

mCherryGGBsMTS

Expression

Bacterial

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pOGG043

Plasmid

#133123

Purpose

Level 0 PU module synthesised as a fragment (pL0M-PU-PsNifH). Synthetic consensus promoter to drive nodule-specific expression in biovars and strains of R. leguminosarum.

Depositor

Philip Poole

Article

Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

PsNifH Synthetic consensus nifH promoter

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pETDUET-GST AO7REwt

Plasmid

#213935

Purpose

GST tagged AO7 encoding aa 126-258 for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Tags

GST

Expression

Bacterial

Mutation

AO7 cloned into the first multiple cloning site o…

Promoter

T7 promoter-1

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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