30,880 results

1. pSM040

   Plasmid

   #128294

   Purpose

   asymmetric plasmid partitioning E. coli - target plasmid

   Depositor

   Matthew Bennett

   Article

   Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

   Insert

   Piq - mRFP, parS
   

   Expression

   Bacterial

   Available Since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJJDuet30

   Plasmid

   #11962

   Depositor

   Hideo Iwai

   Article

   Zuger et al Nat Biotechnol. 2005 Jun . 23(6):736-40.

   Insert

   Ssp DnaE-N
   

   Tags

   GB1

   Expression

   Bacterial

   Available Since

   Sept. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJL1-mTFP1

   Plasmid

   #102635

   Purpose

   In vitro expression of mTFP1 from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   mTFP1
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOPINCDF

   Plasmid

   #41132

   Depositor

   Ray Owens

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   March 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. Fusion of Dengue2 NS2B-NS3 proteins

   Plasmid

   #204792

   Purpose

   Bacterial expression of a fusion of Dengue2 NS2B-NS3 proteins

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Dengue2 NS2B-NS3 fusion
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pDSP32

   Plasmid

   #199374

   Purpose

   Encodes dual cistronic nuclear localized jGCaMP7s and mScarlet-I optimized for C. elegans expression

   Depositor

   Hang Lu

   Insert

   NLS::jGCaMP7s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
   

   Expression

   Bacterial

   Mutation

   no mutations, except added subcellular localizati…

   Available Since

   April 25, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. 5ULO (YWHAZ)

   Plasmid

   #101258

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   YWHAZ (YWHAZ Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 31, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pEBG-NHERF2

   Plasmid

   #28292

   Depositor

   Maria-Magdalena Georgescu

   Article

   Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

   Insert

   NHERF2 (NHERF2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Sept. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

9. MERTK_HUMAN_D0

   Plasmid

   #79705

   Purpose

   This plasmid encodes the kinase domain of MERTK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   MERTK (MERTK Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pSC5GGv2

    Plasmid

    #188604

    Purpose

    pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Expression

    Bacterial and Yeast

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. RBM4_2xRRM-ZNF_pGEX

    Plasmid

    #135154

    Purpose

    Recombinant expression of RBM4 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    RBM4 (RBM4 Human)
    

    Tags

    GST-SBP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXD70DA9-Pct5-DadhABC(A2)

    Plasmid

    #191632

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    Dopamine dehydroxylase
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSNAP-CAPZ

    Plasmid

    #69948

    Purpose

    Simultaneous expression of a His- and SNAP-tagged β1 subunit and untagged α1 subunits of chicken CapZ

    Depositor

    Jeff Gelles

    Article

    Bombardier et al Nat Commun. 2015 Nov 13;6:8707. doi: 10.

    Inserts

    CapZ beta 1 (CAPZB Chicken)
    CapZ alpha 1 (CAPZA1 Chicken)
    

    Tags

    His 6 and SNAP

    Expression

    Bacterial

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-C9ORF80

    Plasmid

    #128418

    Purpose

    Express C9ORF80 in E. coli with a His tag (C-terminal)

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

    Insert

    C9ORF80 (INIP Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-mScarlet-TEV-Strep-His

    Plasmid

    #210823

    Purpose

    Bacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent protein

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    mScarlet
    

    Tags

    TwinStrep, 10xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTetR193-Wasabi

    Plasmid

    #86105

    Purpose

    mWasabi reporter gene gated by temperature-sensitive TetR I193N

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TetRts
    mWasabi
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    I193N

    Promoter

    ptetA and ptetR

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. DNMT3A

    Plasmid

    #25328

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DNMT3A_11 (DNMT3A Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pNCST-mAvicFP1

    Plasmid

    #129509

    Purpose

    Expresses mAvicFP1 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    mAvicFP1
    

    Expression

    Bacterial

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETDuet RagA(D130N)-RagC

    Plasmid

    #99642

    Purpose

    Bacterial expression of RagA(D130N)-RagC

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D130N

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFA6a-mRFP1-kanMX6

    Plasmid

    #133551

    Purpose

    Yeast fluorescent (RFP) vector with PTEF promoter.

    Depositor

    Won-Ki Huh, Erin O'Shea

    Article

    Huh et al Nature. 2003 Oct 16;425(6959):686-91. do

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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