30,245 results

1. pJAK2.D

   Plasmid

   #29465

   Depositor

   Zakaria Hmama

   Article

   Sun et al Plasmid. 2009 Nov . 62(3):158-65.

   Type

   Empty backbone

   Use

   Mycobacteria expression

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Acetamide promoter

   Available since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTriEx-mCherry-LINXa3

   Plasmid

   #73614

   Purpose

   Light-induced nuclear export switch for microscopy in mammalian cells.

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

   Insert

   LINXa3
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Chicken B-Actin

   Available since

   April 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Pet15b-Endosomal Escape Control

   Plasmid

   #186552

   Purpose

   Negative Control confirming the need for a CPP peptide in mediating internalization

   Depositor

   Jean Gariepy

   Article

   Mohammed et al J Control Release. 2012 Nov 28;164(1):58

   Insert

   6H-ETA-GFP
   

   Use

   Tags

   6xHis and EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Pet15b-Internalization and Endosomal Escape

   Plasmid

   #186551

   Purpose

   Internalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cells

   Depositor

   Jean Gariepy

   Article

   Mohammed et al J Control Release. 2012 Nov 28;164(1):58

   Insert

   10R-8His-ETA-EGFP
   

   Use

   Tags

   8xHis and EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. p_SNIPR_target_WT

   Plasmid

   #139465

   Purpose

   T7 RNAP-driven expression of the cognate SNIPR trigger RNA that is complementary to the SNIPR RNA sensor

   Depositor

   Alexander Green

   Article

   Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

   Insert

   Target_WT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHH0103_SNX18-1/1

   Plasmid

   #91369

   Purpose

   Protein expression and purification of human SH3 domain construct SNX18-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SNX18-1/1 (SNX18 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. DYRK2

   Plasmid

   #39031

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   DYRK2 (DYRK2 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-GP-2-ATRX ADD

   Plasmid

   #59698

   Purpose

   Contains histone modification interacting domain (ATRX ADD)

   Depositor

   Albert Jeltsch

   Article

   Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

   Insert

   ATRX ADD (ATRX Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Oct. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAM002

   Plasmid

   #170006

   Purpose

   Encodes PpmrD-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP

   Depositor

   Jeffrey Tabor

   Article

   Brink et al bioRxiv

   Inserts

   Superfolder GFP
   PhoP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLTetO-1 and PpmrD

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Fast-GCaMP6f-RS06

    Plasmid

    #67159

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Badura et al Neurophotonics. 2014 Oct;1(2):025008.

    Insert

    Fast-GCaMP6f-RS06
    

    Use

    Tags

    6xHis, T7 tag, and Xpress tag

    Expression

    Bacterial

    Mutation

    M374Q within GCaMP6f

    Promoter

    T7

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD-D2

    Plasmid

    #37470

    Depositor

    Roger Tsien, Amy Palmer

    Article

    Palmer et al Chem Biol. 2006 May;13(5):521-30.

    Insert

    D2 second generation cameleon (calcium sensor)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ara

    Available since

    Oct. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pGBW-m4046754

    Plasmid

    #145647

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pLQ-KO-tgt50

    Plasmid

    #126578

    Purpose

    CRISPR-Cas9 based efficient genome editing in S. aureus

    Depositor

    Sheng Yang

    Article

    Liu et al Acta Biochim Biophys Sin (Shanghai). 201

    Insert

    Cas9-Pxyltet-sgRNA-Pspac-donor-tgt50
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PTPN22

    Plasmid

    #38933

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PTPN22 (PTPN22 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    G126V, V147G

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pMPMA3dPlac-PfliC-GFPLVA

    Plasmid

    #23342

    Depositor

    Olivia Steele-Mortimer

    Article

    Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

    Insert

    GFP(LVA)
    

    Use

    Tags

    GFP(LVA)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTetQCas-BsaI

    Plasmid

    #170629

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT7C-HSPH1

    Plasmid

    #177662

    Purpose

    Recombinant protein synthesis of human HSPH1

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    HSPH1 (HSPH1 Human)
    

    Use

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-tTALE-mStrawberry-15b

    Plasmid

    #206868

    Purpose

    Express tTALE-mStrawberry

    Depositor

    Kyubong Jo

    Article

    Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

    Insert

    7-mer tTALE-mStrawberry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXW109Hg(RS-RinA-E11)2

    Plasmid

    #123151

    Purpose

    Mercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, PmerT, PhrpLE, PrinA

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. HS-mKate2

    Plasmid

    #105945

    Purpose

    heat shock inducible transgenesis, cytosolic mKate2

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    mKate2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge