30,880 results

1. Human _TFAM_NoMTS_Dimer_pET28a+

   Plasmid

   #60013

   Purpose

   Expresses TFAM No MTS dimerization mutant in bacterial cells

   Depositor

   David Chan

   Article

   Ngo et al Nat Commun. 2014;5:3077. doi: 10.1038/nc

   Insert

   Human TFAM No MTS (TFAM Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Missing 1st 42 aas (Mitochondrial targeting seque…

   Promoter

   T7

   Available Since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEVA12TEC-I

   Plasmid

   #58308

   Purpose

   GeneGuard genomic cassette: for constructing ΔthyA::RBS I repA, Epsilon, FRT-kanR-FRT (medium-copy support for ColE2 COR). Will support pSEVAT7Z.

   Depositor

   Tom Ellis

   Article

   Wright et al ACS Synth Biol. 2014 May 13.

   Insert

   Genomic cassette
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   March 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_Pro-IL-36γ

   Plasmid

   #214345

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL36G (IL36G Human)
   

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOPS0750

   Plasmid

   #115511

   Purpose

   Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA.

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Constitutive promoter pNeo pOGG001

   Available Since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNP1 sfGFP trigger

   Plasmid

   #107359

   Purpose

   Trigger sequence for pNP1 sfGFP toehold switch

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   toehold trigger 7
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRE146

   Plasmid

   #118498

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mCardinal
   

   Expression

   Bacterial

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCON1-ProC.gfp

   Plasmid

   #112517

   Purpose

   ProC driven gfpmut3 expression with synTT

   Depositor

   Olivia Steele-Mortimer

   Article

   Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

   Insert

   ProC gfpmut3 TT
   

   Expression

   Bacterial

   Promoter

   ProC

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKSS

   Plasmid

   #39272

   Depositor

   Peter Kast

   Article

   Kast et al Gene. 1994 Jan 28;138(1-2):109-14.

   Type

   Empty backbone

   Use

   Positive-selection cloning vector

   Expression

   Bacterial

   Available Since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACYC-PLlambda-alkS(WT)

   Plasmid

   #44382

   Depositor

   Hal Alper

   Article

   Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. d

   Insert

   AlkS(WT)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PLlambda

   Available Since

   July 15, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pGEX-PAK2 wt

    Plasmid

    #31671

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    p21-activated protein kinase 2 (PAK2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pTriEx-PA-Rac1-C450A

    Plasmid

    #22025

    Purpose

    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    

    Tags

    6X His

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV domain contains light-insensitive mutation C4…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pET30-2-PHGDH-C234S

    Plasmid

    #107724

    Purpose

    Expression of PHGDH C234S in bacteria

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    PHGDH (PHGDH Human)
    

    Expression

    Bacterial

    Mutation

    C234S

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. p_SNIPR_sensor_LacZ

    Plasmid

    #139464

    Purpose

    T7 RNAP-driven expression of SNIPR RNA with a LacZ reporter

    Depositor

    Alexander Green

    Article

    Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

    Insert

    SNIPR_lacZ
    

    Expression

    Bacterial

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTn7-SCOUT13.G

    Plasmid

    #201001

    Purpose

    pLv1-pUC18R6KT-miniTn7-Tc-sfGFP, TcR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Insert

    superfolder GFP
    

    Expression

    Bacterial

    Promoter

    J23104

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTn7-SCOUT14

    Plasmid

    #201015

    Purpose

    pLv1-pUC18R6KT-miniTn7-aad9, SpR, StR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTn7-SCOUT10

    Plasmid

    #200989

    Purpose

    pL0V-Lv1-pUC18R6KT-miniTn7-GG-Esp3I sites for Antb modules, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEB1-mClover3

    Plasmid

    #103994

    Purpose

    Plasmid encoding mClover3

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mClover3
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S30R, Y39N, …

    Promoter

    proC

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCold I-Hero11

    Plasmid

    #187928

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero11

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero11 (C19orf53 Human)
    

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-His10-SHP1_PTPase (243-541aa)

    Plasmid

    #180812

    Purpose

    Expression of 10*His tagged human SHP1 phosphatase domain (243-541aa)

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    SHP1_PTPase (243-541aa) (PTPN6 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. L1-P941L

    Plasmid

    #15498

    Depositor

    Vance Lemmon

    Article

    Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

    Insert

    L1-P941L (L1CAM Human)
    

    Expression

    Bacterial

    Mutation

    Proline 941 changed to Leucine

    Available Since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only