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TRAV20

Plasmid

#123392

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV20 (TRAV20 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV3-1

Plasmid

#123410

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV3-1 (TRBV3-1 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV8-2

Plasmid

#123376

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV8-2 (TRAV8-2 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV4

Plasmid

#123371

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV4 (TRAV4 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV1-2

Plasmid

#123368

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV1-2 (TRAV1-2 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pJK226

Plasmid

#71125

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pJBL727

Plasmid

#140397

Purpose

Expresses CsoR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-CsoR-T

Tags

6XHis-TEV

Expression

Bacterial

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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PERK 5: PERK.K618R.pGEX4T-1

Plasmid

#21818

Depositor

David Ron

Article

Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

Insert

PERK (537-1114) K618R (Eif2ak3 Mouse)

Tags

GST and Myc (9E10)

Expression

Bacterial

Mutation

PERK Kinase Domain. Amino acids 537-1114. Kinase …

Available Since

March 29, 2010

Availability

Academic Institutions and Nonprofits only

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pMK90

Plasmid

#13323

Depositor

Martin Gellert

Article

Mizuuchi et al J Biol Chem. 1984 Jul 25. 259(14):9199-2

Insert

gyrA

Expression

Bacterial

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
B3-2

Plasmid

#111472

Purpose

translational elongation factor 4

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational elongation factor 4

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pPbuCas13b

Plasmid

#184565

Purpose

Inducible expression of PbuCas13b nuclease in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Cas13b nuclease from Prevotella buccae

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3(D3A,V266E)

Plasmid

#90091

Purpose

Expresses caspase D3A and V266E mutant in bacterial cells

Depositor

Clay Clark

Article

Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

Insert

Caspase 3 (CASP3 Human)

Tags

His tag

Expression

Bacterial

Mutation

D175A D28A D9A V266E

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pED17x11

Plasmid

#134814

Purpose

sfGFP expression plasmid for characterizing qtRNA charging

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

sfGFP-151-TAGA

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

151-TAGA

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET-28a RUVBL2 Delete

Plasmid

#159143

Purpose

Expression and protien purification of a mutated RUVBL2

Depositor

Eric Erquan Zhang

Article

Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

Insert

RUVBL2 (RUVBL2 Human)

Expression

Bacterial

Mutation

amino acid 2-456, Thr127-Glu233 mutated to GPPG

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRKO

Plasmid

#49456

Purpose

loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptions

Depositor

Marc Gartenberg

Article

Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1

Insert

loxP-kanMX-loxP cassette

Use

Cre/Lox

Expression

Bacterial

Promoter

none

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pSB1C3 I0500 LuxI

Plasmid

#107965

Purpose

Arabinose-inducible 3OC6HSL sender plasmid

Depositor

Jim Haseloff

Article

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

luxI

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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PDLIM4_PDZ_1

Plasmid

#103941

Purpose

Protein expression and purification of RIL BGLII PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PDLIM4 (PDLIM4 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBluescript GPX-1

Plasmid

#13038

Depositor

Peter Newburger

Article

Shen et al J Biol Chem. 1993 May 25. 268(15):11463-

Insert

GPX-1 (GPX1 Human)

Expression

Bacterial

Mutation

Additional GCG upstream of codon 11. Codon 92 is …

Available Since

Nov. 7, 2006

Availability

Academic Institutions and Nonprofits only
Caspase-6

Plasmid

#100102

Purpose

Bacterial expression of Caspase-6

Depositor

Jim Wells

Article

Seaman et al Cell Death Differ. 2016 Oct;23(10):1717-

Insert

Caspase-6 (CASP6 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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Paxillin

Plasmid

#186142

Purpose

For expresseion of recombinant paxillin in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Tags

GST

Expression

Bacterial

Mutation

Paxillin C1997A (silent mutation for cloning)

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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