30,249 results

1. pCOLA-T7-WspR:G249A

   Plasmid

   #79163

   Purpose

   Expresses inactive WspR G249A under a T7 promoter

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Insert

   WspR (wspR P. areuginosa)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G249A

   Promoter

   T7

   Available since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. puC19 preACT1(WT)

   Plasmid

   #111323

   Purpose

   for T7 transcription of wt actin pre-mRNA

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZ001 (AacC2c1 locus)

   Plasmid

   #70166

   Purpose

   Expresses AacC2c1 full locus

   Depositor

   Feng Zhang

   Article

   Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

   Insert

   AacC2c1 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pH6HTC_mmHUR-Halo

   Plasmid

   #175311

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   HUR (Elavl1 Mouse)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMJ915v2 + sfGFP

   Plasmid

   #88918

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXPE

   Plasmid

   #15284

   Depositor

   Ron Vale

   Article

   Navone et al J Cell Biol. 1992 Jun . 117(6):1263-75.

   Insert

   ubiquitous kinesin heavy chain (KIF5B Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The insert is missing the 1st 180 bp of 5' u…

   Promoter

   Available since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pGBK CUP1 EGFP

   Plasmid

   #170210

   Purpose

   Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing EGFP

   Depositor

   Bruce Hamilton

   Article

   Hernandez et al bioRxiv

   Insert

   enhanced green fluorescent protein
   

   Use

   Tags

   GAL4 DNA Binding Domain and Myc

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   CUP1

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pExp-PpiB

   Plasmid

   #112572

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8xHis-PpiB and Strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. YARS_pNIC-CTB10H

   Plasmid

   #153059

   Purpose

   Expression construct for producing Avi tagged Tyrosyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   YARS (YARS1 Human)
   

   Use

   Tags

   TEV-Avi-His10

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. USPL1cat-pETM30

    Plasmid

    #85768

    Purpose

    bacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusion

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, catalytic domain (aa 212-514) (USPL1 Human)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    catalytic domain (aa 212-514)

    Promoter

    T7-lac

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOD3-mClover-nat

    Plasmid

    #120328

    Purpose

    Bacterial mClover promoter trap

    Depositor

    James Kirby

    Article

    Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

    Insert

    mClover
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D103N (see comments below)

    Promoter

    (None)

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pELlR1

    Plasmid

    #172569

    Purpose

    Inducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Chloramphenicol Resistance Marker.

    Depositor

    Christopher Reisch

    Insert

    LacI-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLlacO-1

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pELlR2

    Plasmid

    #172555

    Purpose

    Inducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Kanamycin Resistance Marker.

    Depositor

    Christopher Reisch

    Insert

    LacI-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLlacO-1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

    Plasmid

    #101211

    Purpose

    Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/E60C/D273C/N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, E60C, D273C, N497A, R661A, Q695A and…

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

    Plasmid

    #101204

    Purpose

    Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, E60C, D273C, M1-N497, GGS, V713-D1368

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. FDX1

    Plasmid

    #39055

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    FDX1 (FDX1 Human)
    

    Use

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pBAD-pHmGL

    Plasmid

    #191257

    Purpose

    Bacterial expression plasmids for pH sensitive fluorescent protein pHmGL

    Depositor

    Robert Campbell

    Insert

    pHmGL
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJT122

    Plasmid

    #31396

    Depositor

    Christopher Voigt

    Article

    Tabor et al J Mol Biol. 2011 Jan 14. 405(2):315-24.

    Insert

    pJT122
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

19. TwinStrep-SUMO-Pdp1

    Plasmid

    #198320

    Purpose

    For affinity purification of Pdp1 payload

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    TwinStrep-SUMO-Pdp1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Reb_80 aka PAS621

    Plasmid

    #71226

    Purpose

    Reb_1 with RebB(I67T, N84I)

    Depositor

    Pamela Silver

    Article

    Polka et al ACS Synth Biol. 2016 Feb 3.

    Insert

    Reb locus
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    RebB(I67T, N84I)

    Promoter

    Available since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only