31,184 results

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pIM1708-surT-PsurP-agaN-pNW33N

Plasmid

#44009

Depositor

Ichiro Matsumura

Article

Blanchard et al Appl Microbiol Biotechnol. 2014 May 2.

Insert

surT-PsurP-agaN

Expression

Bacterial

Mutation

Deleted EcoRI site (synonymous change, C to T at …

Available Since

May 15, 2014

Availability

Academic Institutions and Nonprofits only

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Sc eRF3∆165N (WT)

Plasmid

#175418

Purpose

Encodes His-MBP-TEV-WT eRF3∆165N

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF3 (SUP35 Budding Yeast)

Tags

His-MBP-TEV

Expression

Bacterial

Mutation

∆165N

Promoter

T7

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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T7RNAP Cyslight V174C

Plasmid

#205078

Purpose

T7RNAP protein expression

Depositor

Smita Patel

Article

Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

Insert

T7RNAP Cyslight V174C (T7p07 Bacteriophage T7)

Expression

Bacterial

Mutation

C125S, C347S, C510S, C515S, C530S, C723S V174C

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-PhoCl

Plasmid

#87688

Purpose

Express PhoCl in E. coli

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PhoCl

Tags

6xHis

Expression

Bacterial

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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human p53-(1-393)-N268D

Plasmid

#24861

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Asparagine 268 mutated to Aspartate

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
PET28a-cpLumiLuc-Rapamycin sensor

Plasmid

#186292

Purpose

Expression Rapamycin sensor in e coli

Depositor

Kirill Alexandrov

Article

Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

Insert

cpLumiLuc-Rapamycin sensor

Tags

HisTag

Expression

Bacterial

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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RRAS2

Plasmid

#38816

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

RRAS2 (Rras2 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pCOM4

Plasmid

#49798

Purpose

ePathBrick vector for pathway engineering in E. coli.

Depositor

Mattheos Koffas

Article

Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

Type

Empty backbone

Tags

S-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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RTA

Plasmid

#64029

Purpose

Expression of Ricin A Chain (RTA) in E. coli

Depositor

Vern Schramm

Article

Ho et al Proc Natl Acad Sci U S A. 2009 Dec 1;106

Insert

Ricin A Chain

Tags

none

Expression

Bacterial

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pETNKI-his-SUMO-RNF168ring (1-189)

Plasmid

#63570

Purpose

Expression of human RNF168 ring domain (residues 1-189) tagged with His-SUMO

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

RNF168 (RNF168 Human)

Tags

6xHis and SUMO3

Expression

Bacterial

Mutation

Ring domain only (residues 1-189)

Promoter

T7

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pBIG1b_10xHisTEVATG5_ATG12

Plasmid

#190861

Purpose

Plasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG5 (ATG5 Human)
ATG12 (ATG12 Human)

Tags

6xHis-TEV

Expression

Bacterial and Insect

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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ReactionC_pBIG1a_ATG7_ATG10

Plasmid

#190860

Purpose

Plasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG7 (ATG7 Human)
ATG10 (ATG10 )

Expression

Bacterial and Insect

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PUM1_PUM_pGEX

Plasmid

#135129

Purpose

Recombinant expression of PUM1 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

PUM1 (PUM1 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pSEVA12TEC-I

Plasmid

#58308

Purpose

GeneGuard genomic cassette: for constructing ΔthyA::RBS I repA, Epsilon, FRT-kanR-FRT (medium-copy support for ColE2 COR). Will support pSEVAT7Z.

Depositor

Tom Ellis

Article

Wright et al ACS Synth Biol. 2014 May 13.

Insert

Genomic cassette

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 6, 2015

Availability

Academic Institutions and Nonprofits only

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pQTEV-PSMD10

Plasmid

#31332

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

PSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin) (PSMD10 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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CBX4

Plasmid

#25237

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

CBX4_02 (CBX4 Human)

Tags

6x His and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 13, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pBS hIhh (CT#402)

Plasmid

#13995

Depositor

Cliff Tabin

Article

Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

Insert

Ihh (IHH Human)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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ftsZ-RFP

Plasmid

#63842

Purpose

This plasmid is designed as a bacterial cell division sensor

Depositor

Howard Salis

Insert

ftsZ, mRFP1 and GFPmut3b

Tags

mRFP1

Expression

Bacterial

Mutation

Gene is translationally fused to RFP

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pProEx Rab1A(S20C) CL

Plasmid

#224272

Purpose

Protein purification of Rab1A (S20C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rab1A (RAB1A Human)

Expression

Bacterial

Mutation

S20C

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLY071f

Plasmid

#227942

Purpose

SICLOPPS precursor for cyclo-CRCVFSG

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Insert

Evolved Ssp SICLOPPS-CRCVFSG

Expression

Bacterial

Mutation

Evolved Ssp split inteins with multiple mutations…

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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