30,880 results

1. pTorPE-G-GECO1.2

   Plasmid

   #32468

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   G-GECO1.2
   

   Use

   Lab constructed

   Tags

   6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

   Expression

   Bacterial

   Mutation

   GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSET-B MT

   Plasmid

   #108855

   Purpose

   Encodes for the fluorescent protein mTurqoise

   Depositor

   Kalina Hristova

   Article

   Sarabipour et al Anal Biochem. 2014 Mar 15;449:155-7. doi

   Insert

   mTurqoise
   

   Expression

   Bacterial

   Available Since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. AKAP13

   Plasmid

   #73232

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   AKAP13 (AKAP13 Human)
   

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 1976-2211

   Promoter

   T7

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. Reb_221 aka PAS628

   Plasmid

   #71233

   Purpose

   Reb_1 with RebA(G90A)

   Depositor

   Pamela Silver

   Article

   Polka et al ACS Synth Biol. 2016 Feb 3.

   Insert

   Reb locus
   

   Tags

   none

   Expression

   Bacterial

   Mutation

   RebA(G53A, G90A)

   Available Since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pQE30-roGFP1-iL

   Plasmid

   #83313

   Purpose

   Inducible expression of redox sensitive GFP in bacteria

   Depositor

   S. James Remington

   Article

   Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

   Insert

   roGFP1-iL
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   ro1(C48S/S147C/Q204C) plus X insertion, iL betwe…

   Promoter

   T5

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pPBO.ACT005

   Plasmid

   #182715

   Purpose

   Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region

   Depositor

   Joseph Schoeniger

   Article

   Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

   Insert

   CasRx crRNA cloning backbone
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   Catalytically deactivated R295A, H300A, R849A, H8…

   Promoter

   pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

   Available Since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. M13cp-dg1 hp

   Plasmid

   #218093

   Purpose

   Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pI

   Depositor

   Kevin Esvelt

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSET FLIPPi-260n

   Plasmid

   #13552

   Depositor

   Wolf Frommer

   Article

   Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

   Insert

   FLIPPi WT
   

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTE103

   Plasmid

   #59398

   Purpose

   MethyloBrick expression vector, fumC promoter; TcR

   Depositor

   Tobias Erb

   Article

   Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PfumC

   Available Since

   Dec. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pH6-spTorA-mCherry

    Plasmid

    #169040

    Purpose

    mCherry preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag in pET28a.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    H6-spTorA-mCherry
    

    Tags

    6xHis and spTorA (signal peptide of E. coli TorA)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPICZa-STE2-1D4

    Plasmid

    #133436

    Purpose

    Mutant form of yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.

    Depositor

    Gerhard Wagner

    Insert

    STE2 (STE2 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    C59S/C252A

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas_5+7_gfp

    Plasmid

    #60595

    Purpose

    encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Cas_5+7_gfp
    

    Expression

    Bacterial

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas_2+5

    Plasmid

    #60594

    Purpose

    encodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Cas_2+5
    

    Expression

    Bacterial

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOPINK-OTUD5 (OTU, aa 171-358)

    Plasmid

    #61415

    Purpose

    Expresses human OTUD5 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD5 (OTUD5 Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-170 and aa 359-571.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRE-Tn5-154

    Plasmid

    #118536

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mOrange2
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJKR-L-cdaR

    Plasmid

    #62558

    Purpose

    Glucarate biosensor. Low copy plasmid that expresses GFP proportional to glucarate concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2B-57

    Plasmid

    #177927

    Purpose

    Expresses yeGFP under control of doxycycline-responsive TET3 promoter (contains three rtTA binding sites)

    Depositor

    Vadim Gladyshev

    Article

    Barre et al (unpublished)

    Insert

    EGFP
    

    Expression

    Bacterial and Yeast

    Promoter

    TET3 (contains three rtTA binding sites)

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-6P-1-DmPABPC1_J

    Plasmid

    #146647

    Purpose

    Bacterial Expression of DmPABPC1

    Depositor

    Elisa Izaurralde

    Insert

    DmPABPC1 (pAbp Fly)
    

    Expression

    Bacterial

    Mutation

    G26S relative to NM_166265.2/NP_725750.1

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. SRpHi2

    Plasmid

    #102542

    Purpose

    Bacterial expression of EGFP fused to the cell penetrating peptide TAT with a GST tag for purification

    Depositor

    Stefan Hell

    Article

    Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

    Insert

    Tat-EGFP
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Top7 V48V

    Plasmid

    #12393

    Depositor

    David Baker

    Article

    Kuhlman et al Science. 2003 Nov 21. 302(5649):1364-8.

    Insert

    Top7 V48V
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only