30,249 results

1. p28BIOH-LIC

   Plasmid

   #62352

   Purpose

   E. coli expression vector with N-terminal AviTag and a C-terminal Hexa-HIS tag

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   6xHis and AviTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-51b(+) ChemoG5

   Plasmid

   #193805

   Purpose

   Protein production of ChemoG5 in bacteria

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoG5
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   EGFP:A206K, T225R; HT7: E143R, E147R, L271E

   Promoter

   T7

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. lacI-Ptac-gusA-pBAV1k

   Plasmid

   #30503

   Depositor

   Ichiro Matsumura

   Article

   Murin et al Appl Environ Microbiol. 2011 Oct 21.

   Insert

   beta-glucuronidase (gusA Escherichia coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOP3MT

   Plasmid

   #112601

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and MBP fusions

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   Avi - 8His-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-4T1-MBP-TEV-NAP1 (WT)

   Plasmid

   #208871

   Purpose

   For expression in E. coli of NAP1

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Use

   Tags

   MBP-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6

   Plasmid

   #192789

   Purpose

   aGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   antiGFP nanobody minimizer
   

   Use

   Tags

   H6 and ybbR

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. GST cdc25c

   Plasmid

   #10969

   Depositor

   Toren Finkel

   Article

   Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

   Insert

   cdc25c (CDC25C Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pET11b-SpyCatcher-M2e

   Plasmid

   #165990

   Purpose

   SpyCatcher-fused influenza M2 ectodomain with removable HisTag

   Depositor

   Vesa Hytönen

   Article

   Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

   Insert

   SpyCatcher-M2e
   

   Use

   Tags

   HisTag and SpyCatcher

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHH0103_PIKFYVE_DEP

   Plasmid

   #109789

   Purpose

   Protein expression and purification of PIKFYVE_DEP

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   PIKFYVE_DEP (PIKFYVE Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET30a-His6-ABP-LOXCATmut

    Plasmid

    #139972

    Purpose

    A control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Mutation in H265A and R268A in LOX (used original…

    Promoter

    T7

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pReporter_5

    Plasmid

    #60566

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET30a-SARS-CoV2-NP-Nt(47-173)

    Plasmid

    #165091

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pBTRCk-pVhb-maACR

    Plasmid

    #215651

    Purpose

    pBBR1 Origin, KanR, with maACR gene under Vhb promoter

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    acyl-CoA reductase
    

    Use

    Tags

    Maltose binding protein and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Vhb

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACYC-pVhb-vfFadBA

    Plasmid

    #215650

    Purpose

    pACYC Origin, CmR, with vfFadBA gene under Vhb promoter

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    thiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Vhb

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK_ToeRepG2_N19_trigger

    Plasmid

    #132747

    Purpose

    T7 RNAP-driven expression of second-generation toehold repressor index 19 trigger RNA

    Depositor

    Alexander Green, Peng Yin

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    ToeRepG2_N19_trigger
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTriEx-mCherry::LANS1

    Plasmid

    #60784

    Purpose

    Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

    Insert

    LANS1
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMM555

    Plasmid

    #68880

    Purpose

    NanoLuc expressed constiutively from PcfxA, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Insert

    NanoLuc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSL0426 (CRISPR with spc3)

    Plasmid

    #130642

    Purpose

    Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    CRISPR(spacer-3)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEX-A-U6-TelgRNA_PuroR

    Plasmid

    #84782

    Purpose

    Expresses telomere-specific sgRNA.

    Depositor

    Heinrich Leonhardt

    Article

    Schmidtmann et al Nucleus. 2016 Sep 27:0.

    Insert

    telomere-specific sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJMD14

    Plasmid

    #138654

    Purpose

    phiOZJ integrating Streptomyces expression vector, intact RDF

    Depositor

    Joshua Blodgett

    Article

    Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PermE* variant sequence from pDA1652

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge