30,880 results

1. HS-mKate2

   Plasmid

   #105945

   Purpose

   heat shock inducible transgenesis, cytosolic mKate2

   Depositor

   Caren Norden

   Article

   Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

   Insert

   mKate2
   

   Expression

   Bacterial

   Available Since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAB035h

   Plasmid

   #79191

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Plac62 gIII, luxAB; Ptet rpoZ-HA4
   

   Expression

   Bacterial

   Promoter

   Plac62; Ptet

   Available Since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-phzAG

   Plasmid

   #141106

   Purpose

   Contains a seven gene phzABCDEFG operon BioBrick

   Depositor

   Martin Buck

   Article

   Clifford et al Chem Sci. 2021 Jan 15;12(9):3328-3338. d

   Insert

   phzABCDEFG
   

   Expression

   Bacterial

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBW-m4046754

   Plasmid

   #145647

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 3C-like proteinase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pGEX-4T1-WDR5

   Plasmid

   #59969

   Purpose

   Bacterial Expression GST-WDR5

   Depositor

   Debu Chakravarti

   Article

   Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

   Insert

   WDR5 (WDR5 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

6. 6C1A (MBD2)

   Plasmid

   #119958

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   MBD2 (MBD2 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pGEX-E6AP Hect

   Plasmid

   #165102

   Purpose

   bacterial expression of a GST fusion of Human E6AP Hect domain

   Depositor

   Arthur Haas

   Article

   Ronchi et al Methods Mol Biol. 2012;832:197-218. doi:

   Insert

   E6AP Hect (UBE3A Human)
   

   Tags

   GST from plasmid.

   Expression

   Bacterial

   Mutation

   E6AP Hect domain Amino acids 495-738 (5'QLNP…

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a(ICOS)

   Plasmid

   #177903

   Purpose

   Bacterial expression of his10-tagged human ICOS Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   ICOS (aa 162-199 only) (ICOS Human)
   

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. proinsulin-msGFP2 (C48S C70S)

   Plasmid

   #218956

   Purpose

   Expresses proinsulin fused to msGFP2 (C48S C70S) in bacteria

   Depositor

   Benjamin Glick

   Insert

   proinsulin-msGFP2 C48S C70S
   

   Tags

   proinsulin

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEThT-SAHS2

    Plasmid

    #90026

    Purpose

    To express His-tagged SAHS2 in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Yamaguchi et al PLoS One. 2012;7(8):e44209.

    Insert

    SAHS2
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMP4518

    Plasmid

    #107778

    Purpose

    Expression of EYFP

    Depositor

    Herman Spaink

    Article

    Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    pLacZ-EYFP in pBBR1-mcs5
    

    Tags

    pLacZ-EYFP

    Expression

    Bacterial

    Promoter

    pLacZ

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28b-MBP-TEV-PWWP_DNMT3A

    Plasmid

    #186970

    Purpose

    Bacterial expression of human DNMT3A PWWP domain (residues 278–427) with His-MBP tag and TEV protease cleavage site.

    Depositor

    Brian Liau

    Article

    Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

    Insert

    DNMT3A PWWP domain (DNMT3A Synthetic, Human)
    

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    PWWP domain (residues 278–427)

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE2-TetOminiCMV-cMyc

    Plasmid

    #136614

    Purpose

    Dox-inducible lentiviral vector expressing mouse cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    cMyc (Myc Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-CDC27 wt

    Plasmid

    #31673

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    cell division cycle 27 homolog (CDC27 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pGEM-HE-Glyco-Venus-bPAC(S27A)

    Plasmid

    #165490

    Purpose

    Xenopus oocyte expression of a photoactivatable adenylyl cyclase derived from bPAC using glycophorin for anchoring to the membrane with reduced dark activity

    Depositor

    Thomas Oertner

    Article

    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

    Insert

    Glyco-Venus-bPAC(S27A)-Myc
    

    Use

    Cdna expression, xenopus oocyte

    Tags

    Myc and Venus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET22b-mEB2-eGFP-6xHis

    Plasmid

    #130982

    Purpose

    Bacterial expression of mouse EB2-GFP-6xHis for protein purification.

    Depositor

    Anne Straube

    Article

    Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

    Insert

    EB2-GFP-6xHis (Mapre2 Mouse)
    

    Tags

    eGFP-6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28-OmpT

    Plasmid

    #68862

    Purpose

    construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

    Depositor

    Neil Kelleher

    Article

    Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

    Insert

    OmpT (ompT )
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHCKAn-phoAp-GRFT

    Plasmid

    #158747

    Purpose

    low phosphate phoAp induction of Griffithsin

    Depositor

    Michael Lynch

    Article

    Decker et al Front Bioeng Biotechnol. 2020 Aug 21;8:1

    Insert

    phoAp Griffithsin
    

    Expression

    Bacterial

    Promoter

    phoA

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET51b(+)_LAMA-G97 (for GFP)

    Plasmid

    #130715

    Purpose

    Plasmid encoding the bacterial expression of LAMA-G97 for GFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    LAMA-G97
    

    Tags

    Hisx10

    Expression

    Bacterial

    Mutation

    Deleted first Met, Ala and Last Ser from the Enha…

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZH514

    Plasmid

    #102667

    Purpose

    Constitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Expression

    Bacterial

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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