30,249 results

1. pSC301

   Plasmid

   #31851

   Depositor

   Yossef Av-Gay

   Article

   Cowley et al Gene. 2001 Feb 21;264(2):225-31.

   Insert

   GFP (including ATG start codon)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   SOD

   Available since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pFTK020

   Plasmid

   #171292

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   PalcA syn_NoCrea (ANIA_08979) promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Promoter

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDB24

   Plasmid

   #91959

   Purpose

   empty expression vector for 10His-SUMO tag protein purification

   Depositor

   Christopher Bahl

   Type

   Empty backbone

   Use

   Tags

   10-His and Smt3

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   T7

   Available since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOPINEneo-3C-GFP

   Plasmid

   #53534

   Purpose

   pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system

   Depositor

   Ray Owens

   Insert

   GFP
   

   Use

   Tags

   GFP-His8

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS chick islet1 [TJ#44]

   Plasmid

   #16273

   Depositor

   Thomas Jessell

   Insert

   islet1 (ISL1 Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pGEX2TK-Num1-PH

   Plasmid

   #71369

   Purpose

   bacterial expression of PH domain for phosphoinositide binding assay

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Num1-PH
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa2563-2692

   Promoter

   tac

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

7. Reb_140 aka PAS625

   Plasmid

   #71230

   Purpose

   Reb_1 with RebA(S88P)

   Depositor

   Pamela Silver

   Article

   Polka et al ACS Synth Biol. 2016 Feb 3.

   Insert

   Reb locus
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   RebA(S88P)

   Promoter

   Available since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pTn7xKS-dTomato

   Plasmid

   #117395

   Purpose

   Tn7 tagging vector pTn7xKS with dTomato expression scaffold

   Depositor

   Karen Guillemin

   Article

   Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

   Insert

   dTomato
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MBP-His6-TEV-MMS2

   Plasmid

   #25465

   Depositor

   Cynthia Wolberger

   Article

   Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

   Insert

   MMS2 (MMS2, UBC13 Budding Yeast)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 21, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pECT3

    Plasmid

    #75450

    Purpose

    Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS-SK-QS

    Plasmid

    #24370

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    QS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. XRCC1-c003

    Plasmid

    #173090

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    XRCC1 (XRCC1 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E315-P406

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX CrkL-SH2

    Plasmid

    #46419

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Crkl (CRKL Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 9-108

    Promoter

    Tac

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCellFree_G06_ZIKV NS5

    Plasmid

    #145358

    Purpose

    Cell-free expression vector for Zika virus NS5 protein with C-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS5
    

    Use

    Tags

    mCherry and 8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHH0103_CSK-1/1

    Plasmid

    #91542

    Purpose

    Protein expression and purification of human SH3 domain construct CSK-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CSK-1/1 (CSK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. SRpHi1

    Plasmid

    #102541

    Purpose

    Bacterial expression of EYFP fused to the cell penetrating peptide TAT with a GST tag for purification

    Depositor

    Stefan Hell

    Article

    Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

    Insert

    Tat-EYFP
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMOD3-mWasabi-nat

    Plasmid

    #120331

    Purpose

    Bacterial mWasabi promoter trap

    Depositor

    James Kirby

    Article

    Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

    Insert

    mWasabi
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    (None)

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIntegrase_12

    Plasmid

    #60583

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. DinoIII

    Plasmid

    #128100

    Purpose

    Dinoflagellate expression vector backbone. XbaI and BglII restriction sites for gene insertion.

    Depositor

    Senjie Lin

    Article

    Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m

    Insert

    RNA complex & upstream/downstream of LHC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Inserts are potential promoters

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC19-T7-Top-T

    Plasmid

    #87309

    Purpose

    Expresses the "Top" transcript of the Split-Broccoli system under the control of the T7 promoter.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system "Top" transcript
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge