30,880 results

1. pCCL-ReDNAP (Y427A)

   Plasmid

   #52346

   Purpose

   CEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)

   Depositor

   Chang Liu

   Article

   Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

   Insert

   Recoded TP-DNAP
   

   Expression

   Bacterial and Yeast

   Promoter

   REV1

   Available Since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-ATAD2-plx303

   Plasmid

   #188635

   Purpose

   ATAD2 overexpression

   Depositor

   Lorenz Studer

   Article

   Baggiolini et al Science. 2021 Sep 3;373(6559):eabc1048.

   Insert

   ATAD2 (ATAD2 Human)
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. R26-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP

   Plasmid

   #180153

   Purpose

   td-sfGFP dre reporter with Rosa26 recombineering homology arms

   Depositor

   Mario Capecchi

   Insert

   td-sfGFP
   

   Use

   Mouse Targeting; Dre/rox

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpyCas9(WT)

   Plasmid

   #101044

   Purpose

   Express Streptococcus pyogenes Cas9

   Depositor

   Zhiwei Huang

   Article

   Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

   Insert

   SpyCas9
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Clover-pBAD

   Plasmid

   #54575

   Purpose

   Localization: Protein Expression Vector , Excitation: 505, Emission: 515. This plasmid encodes Clover H231L based on reference from Bajar et. al. Sci Rep. 2016.

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Type

   Empty backbone

   Tags

   6xHis and Clover

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pXPE

   Plasmid

   #15284

   Depositor

   Ron Vale

   Article

   Navone et al J Cell Biol. 1992 Jun . 117(6):1263-75.

   Insert

   ubiquitous kinesin heavy chain (KIF5B Human)
   

   Expression

   Bacterial

   Mutation

   The insert is missing the 1st 180 bp of 5' u…

   Available Since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pGI3EM09

   Plasmid

   #135482

   Purpose

   Agrobacterium T-DNA with SpunHsp70pr driving hph-GFP

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   SpunHsp70pr hph-GFP ADH1t
   

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Promoter

   SpunHsp70pr

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNP1 eforRed toehold switch

   Plasmid

   #107353

   Purpose

   Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter when pNP1 eforRed trigger is co-expressed

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   toehold switch 1 + eforRed
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. 4LEC

   Plasmid

   #60091

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   METTL21A:JMC072-B07:C227766 (METTL21A Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available Since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pTriEX-Antenna-GDI Rac1

    Plasmid

    #83359

    Purpose

    Expression of Antenna-Rac1

    Depositor

    Klaus Hahn

    Article

    Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

    Insert

    Rac1 (RAC1 Human)
    

    Tags

    FRET Antenna (mCerulean-cpVenus) and His

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-His6-Sumo-MBP-CfaN

    Plasmid

    #126016

    Purpose

    Bacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)

    Depositor

    Thomas Muir

    Insert

    His5-Sumo-MBP-CfaN
    

    Tags

    5xHis-SUMO

    Expression

    Bacterial

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTriEx-mCherry-LINXa3

    Plasmid

    #73614

    Purpose

    Light-induced nuclear export switch for microscopy in mammalian cells.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    LINXa3
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    Chicken B-Actin

    Available Since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. p7XC3H

    Plasmid

    #47065

    Purpose

    FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site and 10x His tag

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site) and decaHis (…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. PTPRK

    Plasmid

    #38968

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PTPRK (PTPRK Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pGBW-m4046747

    Plasmid

    #149249

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    May 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pTH1-SpSrtA

    Plasmid

    #64987

    Purpose

    Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    S. pyogenes sortase A
    

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-81

    Promoter

    T7 promoter

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKM631

    Plasmid

    #17727

    Depositor

    David Waugh

    Article

    Kapust et al Biochem Biophys Res Commun. 2002 Jun 28.

    Insert

    NusG (nusG Aquifex aeolicus)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Available Since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM6-Amp-T7-roGFP

    Plasmid

    #202464

    Purpose

    Expresses redox sensitive GFP (roGFP) with T7 induction

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    reduction-oxidation sensitive green fluorescent protein
    

    Tags

    6X-His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACYCDUET-StrepSUMO-DaPFOR

    Plasmid

    #217819

    Purpose

    Expresses DaPFOR in E. coli

    Depositor

    Pamela Silver

    Article

    Ng et al ACS Chem Biol. 2024 Feb 16;19(2):380-391

    Insert

    pACYCDUET-StrepSUMO-DaPFOR
    

    Tags

    StrepSUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. p4286

    Plasmid

    #198152

    Purpose

    bacterial expression of sseJ::HaloTag fusion

    Depositor

    Michael Hensel

    Insert

    PsseJ sseJ::L16::HaloTag::HA (sseJ Salmonella enterica serovar Typhimurium)
    

    Tags

    HaloTag-HA

    Expression

    Bacterial

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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