30,249 results

1. pGEX Slp76-SH2

   Plasmid

   #46503

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Slp76 (LCP2 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 417-533

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-EF1_Cre

   Plasmid

   #201198

   Purpose

   AAV vector for cre expression under EF1 promoter

   Depositor

   Tetsuo Yamamori

   Article

   Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

   Inserts

   cre
   EF1alpha promoter
   

   Use

   AAV

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   nuclear targeting cre

   Promoter

   N/A and hEF1alpha promoter

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTricHis-PLY

   Plasmid

   #160537

   Purpose

   Expression of inserted PLY variant

   Depositor

   Doryen Bubeck

   Article

   Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

   Insert

   PLY-WT
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CP2-TCC

   Plasmid

   #179679

   Purpose

   Complementary plasmid

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   [sd2] T7 RNAP-TCC linker-degron
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNSSCP

   Plasmid

   #183188

   Purpose

   PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T

   Depositor

   Hsiao-Chun Huang

   Article

   Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

   Inserts

   DivIVA and superfolder GFP fusion protein
   SpmX450 and T7 181+ fusion protein
   T7 RNAP 1-179 variant and SpmX450 fusion protein
   mRFP1 and popZ fusion protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHL 1903

   Plasmid

   #53020

   Purpose

   AmpR + fimS::gfpAAV::Asp terminator + CamR + ColE1

   Depositor

   Han Lim

   Article

   Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   AAV, Asp terminator, and fimS

   Expression

   Bacterial

   Mutation

   Promoter

   fimS

   Available since

   May 14, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pAV10.KN

   Plasmid

   #63209

   Purpose

   pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJMP1104

   Plasmid

   #119248

   Purpose

   knockdown pqsC in P. aeruginosa

   Depositor

   Jason Peters, Oren Rosenberg, Carol Gross

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA pqsC (P. aeruginosa)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQE80l A-CC-A

   Plasmid

   #196478

   Purpose

   Protein expression of SpyTag-ELP-CarHCC-ELP SpyTag, with GRGD site linked with (GGGGS)2 before CarHCC and after first ELP

   Depositor

   Fei Sun

   Article

   Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

   Insert

   A-CC-A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28b-PaODH

    Plasmid

    #110802

    Purpose

    Expresses PaODH and opine dehydrogenase from Pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

    Insert

    Pa4835
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET15TV-L-SaNAS

    Plasmid

    #110803

    Purpose

    Expresses SaNAS a nicotianamine synthase from Staphylcoccus aureus

    Depositor

    Audrey Lamb

    Article

    McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

    Insert

    SAV2469 (SAV2469 )
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28b-PaNAS

    Plasmid

    #110801

    Purpose

    Expresses PaNAS nicotianamine synthase from pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

    Insert

    Pa4836
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET15TV-L-SaODH

    Plasmid

    #110804

    Purpose

    Expresses SaODH and opine dehydrogenase from Staphylococcus aureus

    Depositor

    Audrey Lamb

    Article

    McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

    Insert

    SAV2468 (SAV2468 )
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. bactin:GFP-PCNA

    Plasmid

    #105979

    Purpose

    transgenesis, ubiquitous expression

    Depositor

    Caren Norden

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHH0103_TNK2-1/1

    Plasmid

    #91377

    Purpose

    Protein expression and purification of human SH3 domain construct TNK2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    TNK2-1/1 (TNK2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pWA PAS-E BAD K-GAFm

    Plasmid

    #39866

    Depositor

    Vladislav Verkhusha

    Article

    Filonov et al Chem Biol. 2013 Jul 23. pii: S1074-5521(

    Inserts

    iRFP protein PAS domain
    iRFP protein GAF domain (mutated)
    

    Use

    Tags

    E coil and K coil

    Expression

    Bacterial

    Mutation

    F165Y, D232Y, W308R

    Promoter

    arabinose inducible and rhamnose inducible

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRAU171

    Plasmid

    #86835

    Purpose

    bacterial expression of anti-CRISPR protein AcrIIA2

    Depositor

    Joseph Bondy-Denomy

    Article

    Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

    Insert

    acrIIA2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBSMrnaStrp

    Plasmid

    #67508

    Purpose

    Express the tRNAMet scaffold with the streptavidin aptamer tag

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

    Insert

    modified tRNAMet with streptavidin aptamer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

19. TLS 1: hTLS.pBSKS(+)

    Plasmid

    #21827

    Depositor

    David Ron

    Article

    Crozat et al Nature. 1993 Jun 17;363(6430):640-4.

    Insert

    TLS (FUS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HC89 pComb3xss anti-Homer1 nanobody

    Plasmid

    #134719

    Purpose

    Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-Homer1 (Mouse) recombinant llama nanobody HC89 (Homer1 L. glama (llama))
    

    Use

    Tags

    6xHis-HA

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge