33,128 results
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Plasmid#71755PurposeHis-TEV-Aurein1.2+36GFP-LPETG-His expression in E.ColiDepositorInsertAurein1.2_(pos36_GFP)-LPETG-His
Tags6XHis, Sortase (LPETG), and TEV ProteaseExpressionBacterialAvailable SinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
(GGS)9_(+36GFP)-Cre.ape
Plasmid#71747Purpose+36-GFP-Cre fusion expression in E.ColiDepositorInsert(GGS)9_+36GFP-Cre
Tags6XHisExpressionBacterialAvailable SinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hDcp2 E147Q, E148Q
Plasmid#72215Purposebacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)DepositorAvailable SinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc2-STRNDHFR
Plasmid#63960PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneTagsStrep II (STR); TEV cleavableExpressionBacterialPromoterT7Available SinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc2-DHFRHIS
Plasmid#63967PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneTags6xHisExpressionBacterialPromoterT7Available SinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP6
Plasmid#71469PurposeEncodes human full length SENP6 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 6 (SENP6 Human)
TagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-FLAGNDHFR
Plasmid#63979PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneTagsFLAG; TEV cleavableExpressionBacterialPromoterT7Available SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
p045
Plasmid#70057PurposeCo-expresses Syt1-SNARE37aa-linker complex with pDC184DepositorInsertsC2AB-linker-SNAP-25_C
SNAP-25_N
TagsNo tagsExpressionBacterialPromoterT7Available SinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP-P9
Plasmid#69668PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR ugi APOBEC1
ExpressionBacterialPromoterE. coli PBADAvailable SinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
5C7M (ITCH)
Plasmid#69966PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertITCH:SDC094-D06:C29524 (ITCH Human)
TagsHis and TEV cleavage siteExpressionBacterialPromoterT7-lacOAvailable SinceOct. 7, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
5C7J
Plasmid#69965PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertNEDD4:SDC094-F02:C29544 (NEDD4 Human)
TagsHis and TEV cleavage siteExpressionBacterialPromoterT7-lacOAvailable SinceOct. 7, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAT895
Plasmid#68934PurposeInt12 expressed constitutively from PAM4 and the rpiL* RBS, pNBU2 backbone, AmpRDepositorInsertInt12
ExpressionBacterialAvailable SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT891
Plasmid#68932PurposeInt8 expressed constitutively from PAM4 and the rpiL* RBS, pNBU2 backbone, AmpRDepositorInsertInt8
ExpressionBacterialAvailable SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT796
Plasmid#68929PurposeNanoLuc expressed constiutively from PAM4 and rpiL* RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
ExpressionBacterialAvailable SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMON-HsNMT2
Plasmid#67476PurposeExpression of human N-myristoyltransferase 2 for N-myristoylation of ARF family proteins in bacteriaDepositorInsertHsNMT2 (NMT2 Human)
ExpressionBacterialAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARF6
Plasmid#67314PurposehArf6 with stop codon in Gateway Entry vectorDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT892
Plasmid#68933PurposeInt9 expressed constitutively from PAM4 and the rpiL* RBS, pNBU2 backbone, AmpRDepositorInsertInt9
ExpressionBacterialAvailable SinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRII-1.1-1.10a
Plasmid#69589PurposeOT-II TCR a chain cDNA in pCRIIDepositorInsertTCR alpha clone 1.1 (Tcra Mouse)
ExpressionBacterialAvailable SinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
MBP-GTSE1(long)-His6
Plasmid#69110PurposeFor bacterial expression of recombinant human GTSE1 (longer isoform) tagged with MBP and His.DepositorAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
BASIC_7_J23101-RBS34-mCherry-B0015
Plasmid#68141Purposestorage vector for BASIC part: RFP expression cassetteDepositorInsertJ23101-RBS34-mcherry-B0015
ExpressionBacterialAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only