34,239 results

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pET22b_PfSSB

Plasmid

#103000

Purpose

The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB

Depositor

Martin Webb

Publication

Chisty et al PLoS One. 2018 Feb 21;13(2):e0193272. do

Insert

PfSSB

Expression

Bacterial

Promoter

T7

Available since

May 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX-SF3B1-NTerm

Plasmid

#97425

Purpose

Expresses human SF3B1 (AA 1-500) with N-term GST tag for inducible expression in E.coli

Depositor

Alex Minella

Publication

Murthy et al J Biol Chem. 2018 May 15. pii: RA118.001

Insert

SF3B1-N-term (AA1-500)

Tags

GST

Expression

Bacterial

Mutation

Silent mutation at nt876, aa289 (AGG to AGA)

Promoter

tac

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMB1-spect-PthrC-eGFP

Plasmid

#107408

Purpose

This plasmid contain promoter PthrC, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

Depositor

Kang Zhou

Publication

Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

Insert

eGFP

Expression

Bacterial

Promoter

PthrC

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDB026e

Plasmid

#109261

Purpose

Accessory plasmid for PACE of MjTyrRS variants

Depositor

David Liu

Publication

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp_pIII(P29*, Y184*); luxAB; PproK_TyrT(Opt, CUA);

Expression

Bacterial

Mutation

amber codon at position 29 and 184 of gIII

Promoter

Ppsp and PproK

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAB094e

Plasmid

#79245

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Publication

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PBAD rpoZ-HA4 (Y87A); PC araC

Expression

Bacterial

Promoter

PBAD; PC

Available since

May 12, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-NmCas9-Flag

Plasmid

#109030

Purpose

Wild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Publication

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Tags

Flag Tag

Expression

Bacterial

Promoter

T7

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pIB167

Plasmid

#90190

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pspac promoter

Depositor

Indranil Biswas

Publication

Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

Type

Empty backbone

Tags

His-tag and Strep-tag

Expression

Bacterial

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pWT018a

Plasmid

#107885

Purpose

R3 plasmid. Contains PBAD-EGFP and is part of the CAMERA system for rewritable multi-event analog recording in bacteria

Depositor

David Liu

Publication

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PBAD-EGFP

Expression

Bacterial

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pBL132

Plasmid

#104333

Purpose

Expresses taz from a LacI regulated promoter under inducible IPTG control.

Depositor

Jeffrey Tabor

Publication

Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

Insert

Taz

Expression

Bacterial

Promoter

PtacI

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT046a

Plasmid

#107897

Purpose

W2.2, W2.4-1 plasmid. Contains PTetO-SD8-BE2, PBAD-sgRNA3 and is part of CAMERA 2.2 and 2.4

Depositor

David Liu

Publication

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PBAD-sgRNA3

Expression

Bacterial

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmMaple2-CAM

Plasmid

#101149

Purpose

Fluorescent protein for superresolution imaging

Depositor

Xiaowei Zhuang

Publication

Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

Insert

E. coli codon optimized mMaple2

Expression

Bacterial

Promoter

pBAD

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBBR6k-J23109-GFPuv

Plasmid

#106392

Purpose

pBBR6k-GFPuv with Anderson promoter J23109 expressing lacI

Depositor

Brian Pfleger

Publication

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

LlacO-1

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBBR6k-J23101-GFPuv

Plasmid

#106390

Purpose

pBBR6k-GFPuv with Anderson promoter J23101 expressing lacI

Depositor

Brian Pfleger

Publication

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

LlacO-1

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJ4M/TDP-43 S48E

Plasmid

#104481

Purpose

bacterial expression of TDP-43 S48E

Depositor

Nicolas Fawzi

Publication

Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

TDP-43 (TARDBP Human)

Tags

MBP-6xHis

Expression

Bacterial

Mutation

S48E

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET_BGL2

Plasmid

#101911

Purpose

Expresses a β-glucosidase gene from Thermobaculum terrenum in E. coli under the control of the pET system.

Depositor

Steve Shih

Publication

Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944

Insert

β-glucosidase

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7+LacO

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHH0103_CTTN-1/1

Plasmid

#91550

Purpose

Protein expression and purification of human SH3 domain construct CTTN-1/1

Depositor

Sachdev Sidhu

Publication

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CTTN-1/1 (CTTN Human)

Expression

Bacterial

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_GRB2-1/2

Plasmid

#91480

Purpose

Protein expression and purification of human SH3 domain construct GRB2-1/2

Depositor

Sachdev Sidhu

Publication

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

GRB2-1/2 (GRB2 Human)

Expression

Bacterial

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SNX18-1/1

Plasmid

#91369

Purpose

Protein expression and purification of human SH3 domain construct SNX18-1/1

Depositor

Sachdev Sidhu

Publication

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SNX18-1/1 (SNX18 Human)

Expression

Bacterial

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRSETa-mMaple3-V157I

Plasmid

#98577

Purpose

photoconvertible fluorescent protein mMaple3-V157I in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

Depositor

Ulrike Endesfelder

Publication

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

mMaple3-V157I

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAP1698-4

Plasmid

#105242

Purpose

mammalian eGFP

Depositor

Geraldine Seydoux

Publication

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

eGFP

Expression

Bacterial

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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