30,256 results

1. NM-01

   Plasmid

   #69799

   Purpose

   Bacterial expression of Cas9 with His, MBP, and Strep tags

   Depositor

   Gerard Cagney

   Insert

   Cas9
   

   Use

   Tags

   HIS, MBP, and StrepII

   Expression

   Bacterial

   Mutation

   Aspartate 10 to Alanine (D10A) and Histidine 840 …

   Promoter

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pColdII-SIRPT1-Loxodonta Africana

   Plasmid

   #75351

   Purpose

   Nt His-tag Loxodonta Africana 89-1368

   Depositor

   Kalle Gehring

   Insert

   Sacsin Loxodonta Africana 89-1368
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFAB815

   Plasmid

   #47854

   Purpose

   BIOFAB reporter plasmid for measuring rnpB T1 termination efficiency

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   rnpB T1 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLTetO9

   Available since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. yqhD-RFP

   Plasmid

   #63845

   Purpose

   This plasmid is designed as a bacterial oxidative stress sensor

   Depositor

   Howard Salis

   Insert

   yqhD, mRFP1 and GFPmut3b
   

   Use

   Tags

   mRFP1

   Expression

   Bacterial

   Mutation

   Gene is translationally fused to RFP

   Promoter

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSNA

   Plasmid

   #135192

   Purpose

   Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassette

   Depositor

   Hongqing Fang

   Article

   Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

   Insert

   I-SceI lamba Red
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   araBAD

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. TBXTA-c043

   Plasmid

   #139766

   Purpose

   Protein expression/Streptavidin pull-down

   Depositor

   Opher Gileadi

   Insert

   TBXT, DNA-binding domain, WT (TBXT Human)
   

   Use

   Tags

   Avi-tag (Biotin) and His6-TEV

   Expression

   Bacterial

   Mutation

   Contains only amino acids E41- D225

   Promoter

   T7

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. C331

   Plasmid

   #141123

   Purpose

   RhlI expressing plasmid

   Depositor

   Matthew Bennett

   Article

   Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

   Insert

   RhlI (rhlI )
   

   Use

   Tags

   ssrA degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   engineered lac promoter

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX-4T-1-β4 hinge-ear

   Plasmid

   #196692

   Purpose

   Expresses GST-AP-4 β4 hinge-ear (residues 534-739) in bacteria

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

   Insert

   AP-4 β4 hinge-ear (534-739) (AP4B1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX SHP-2(N)-SH2

   Plasmid

   #46498

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   SHP-2(N) (PTPN11 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1-104

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDZ585 pKAN 2x-mCherry

    Plasmid

    #72236

    Purpose

    Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 2x-mCherry

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    2x-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EcRho

    Plasmid

    #113121

    Purpose

    Expresses E. coli Rho

    Depositor

    James Berger

    Article

    Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

    Insert

    Rho
    

    Use

    Tags

    MGH

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHS1929

    Plasmid

    #205968

    Purpose

    JAGBLH010000220 Cas8-HNH crRNA expression in pCOLADuet-1

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8-HNH crRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. His6x-EX-HA-FRB-MBD_pYFJ16

    Plasmid

    #120917

    Purpose

    Expresses His6-EX-HA-FRB-MBD in bacteria for protein purification

    Depositor

    Alice Ting

    Article

    Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

    Insert

    His6-EX-HA-FRB-MBD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28b-LanCL2 (6xHis-LanCL2)

    Plasmid

    #73407

    Purpose

    Expresses His tagged LanCL2

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    LanCL2 (LANCL2 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

15. mNG-sfTq2

    Plasmid

    #124218

    Purpose

    Cytoplasmic mNeongreen superfolder mTurquoise2 tandem, ~60 % energy transfer. pNM044 - pSAV057-mNG-sfTq2

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    mNG-sfTq2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Ikaros

    Plasmid

    #11261

    Depositor

    Lisa Steiner

    Article

    Willett et al Dev Dyn. 2001 Dec . 222(4):694-8.

    Insert

    ikaros (ikzf1 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pMS1: pHelper(AvCAST)_AvPSP1

    Plasmid

    #168133

    Purpose

    Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST minimal CRISPR array (with spacer for AvPSP1)
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    AvTnsD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hOtub1

    Plasmid

    #26959

    Depositor

    Cynthia Wolberger

    Article

    Wang et al J Mol Biol. 2009 Mar 6. 386(4):1011-23.

    Insert

    Otub1 (OTUB1 Human)
    

    Use

    Tags

    His6 (TEV cleavable)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pHH0103_ALDOA_Glycolytic

    Plasmid

    #109854

    Purpose

    Protein expression and purification of ALDOA_Glycolytic

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    ALDOA_Glycolytic (ALDOA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTarget-RFP-GFP operon

    Plasmid

    #192279

    Purpose

    RFP GFP fusion protein expressed under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    RFP GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacIq

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge