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Plasmid#174811PurposeBacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomainDepositorInsertPARP1-CATdeltaHD (PARP1 Human)
UseTags6xHisExpressionBacterialMutationResidues 678-787 replaced by eight-residue linker…PromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mAmetrine-CNA35
Plasmid#61604Purposebacterial expression of N-terminal fusion protein of CNA35 with mAmetrineDepositorInsertmAmetrine and CNA35
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPM2213
Plasmid#167553PurposeEncodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12DepositorInsertrfb region of Shigella flexneri serotype 2a
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
His6TEV-UbG76C
Plasmid#75299PurposeBacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV proteaseDepositorInserthuman Ubiquitin mutant G76 to C
UseTagsHisExpressionBacterialMutationchanged Glycine 76 to CystinePromoterT7Available sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRAD1
Plasmid#47697PurposeGeneral cloning vector for use in E. coli or D. radioduransDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterrepUAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
PAJ698
Plasmid#178947PurposeExpresses P2 tail fiber (gpH) in fusion with S16 tail fiber (gp37)DepositorInsertChimeric Tail Fiber Fusion P2-gpH and S16 gp37
UseUnspecifiedTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
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Nck
Plasmid#186133PurposeFor expression of recombinant Nck in bacteria cells.DepositorInsertNCK1 (NCK1 Human)
UseTagsGSTExpressionBacterialMutationAmino acid residues 1-377, with mutations: C139S,…PromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-IPP1-His
Plasmid#124137PurposeBacterial expression plasmid for PURE system componentsDepositorInsertInorganic pyrophosphatase (IPP1 Budding Yeast)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PAS528 (TJF059)
Plasmid#65602PurposepETDuet-1 Containing FadD-HisDepositorInsertFadD
UseTags6x HisExpressionBacterialMutationPromoterT7 lacOAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b-zDjCas13d-His
Plasmid#234481PurposePlasmid for bacterial expression and purification of DjCas13d protein (zebrafish codon-optimized)DepositorInsertDjCas13d
UseCRISPRTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNC02
Plasmid#233644PurposeFor bacterial expression of sfGFP c-terminally tagged with VitelloTagB.DepositorInsertsfGFP with c-terminal VitelloTagB
UseTags6-HisExpressionBacterialMutationPromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-T4uvsW
Plasmid#228201PurposeExpression in BL21(DE3) of T4 3' to 5' helicase/single-strand DNA annealaseDepositorInsertuvsW
UseTagshexahistidine and 10 amino acid linkerExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas9-CR4
Plasmid#62655PurposeCas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetRDepositorInsertCas9 nuclease
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tn7 helper plasmid
Plasmid#141161PurposeTemperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoterDepositorInsertTn7 transposon machinery
UseTagsExpressionBacterialMutationPromoterArabinose-inducible pBADAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCJ190
Plasmid#162667PurposepET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCitrine-pBAD
Plasmid#54723PurposeLocalization: Protein Expression Vector , Excitation: 516, Emission: 529DepositorTypeEmpty backboneUseTags6xHis and mCitrineExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-Bxb1-His
Plasmid#123132PurposeBacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.DepositorInsertBxb1 integrase
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-GluRS-His
Plasmid#124109PurposeBacterial expression plasmid for PURE system componentsDepositorInsertGlutamyl-tRNA ligase (gltX Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTR47m4-GFP
Plasmid#102436PurposeFormaldehyde biosensor plasmid with sGFP reporter and modified frm promoterDepositorInsertsFormaldehyde repressor protein (frmR)
Superfolder GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only