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Bacterial Expression

30,535 results

Showing: 921 - 940 of 30535 results

  2. pERM2-nhpSer

    Plasmid
    #201922
    Purpose
    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.
    Depositor
    Richard Cooley, Ryan Mehl
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    E412P, E414F, T417K, P495M, I496W, F529S and H67R…
    Promoter
    GlnS (constitutive), OXB20 (constitutive), lpp (c…
    Available Since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201922-plasmid-map-sequence-id-395413

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  4. pKAR5

    Plasmid
    #149461
    Purpose
    Broad-Host range expression vector, RK2 and pUC origin, AraC activator/repressor, PBAD promoter, mRFP, Gentamicin Resistance Marker.
    Depositor
    Christopher Reisch
    Article
    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629
    Insert
    AraC-mRFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available Since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    149461-plasmid-map-sequence-id-311913

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  5. pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)

    Plasmid
    #114070
    Purpose
    T7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    bacteria codon optimized LbCas12a with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114070-plasmid-map-sequence-id-220511

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  6. pL99

    Plasmid
    #78577
    Purpose
    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains inducible PnitA-NitR system
    Depositor
    Xuming Mao
    Article
    Sun et al Annals of Microbiology Dec 2012, Volume
    Type
    Empty backbone
    Use
    Streptomyces expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    inducible PnitA-NitR system
    Available Since
    May 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    78577-plasmid-map-sequence-id-263121

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  11. pET28a Gx-NL

    Plasmid
    #169105
    Purpose
    Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.
    Depositor
    Maarten Merkx
    Article
    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66
    Insert
    protein G - NanoLuc
    Use
    Tags
    his-tag and strep-tag
    Expression
    Bacterial
    Mutation
    Ala 24 stop
    Promoter
    T7
    Available Since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    169105-plasmid-map-sequence-id-335658

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Showing: 921 - 940 of 30535 results