30,810 results

1. pGex-4T-2_GEC1

   Plasmid

   #73945

   Purpose

   For recombinant expression of human GEC1/GABARAPL1 in E. coli

   Depositor

   Dieter Willbold

   Article

   Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

   Insert

   GEC1 (GABARAPL1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pSrpR-S3

   Plasmid

   #74693

   Purpose

   SrpR-S3 gate

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

   Insert

   PTac-RBSS3-SrpR-PSrpR-YFP
   

   Expression

   Bacterial

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGBW-m4136464

   Plasmid

   #149534

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pBTK509

   Plasmid

   #110610

   Purpose

   BTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Expression

   Bacterial

   Available Since

   Aug. 21, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. PANK1

   Plasmid

   #25517

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PANK1 (PANK1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 24, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pKCyR5

   Plasmid

   #149463

   Purpose

   Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CymR(AM)-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PCymRC

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. puC19 preACT1(WT)

   Plasmid

   #111323

   Purpose

   for T7 transcription of wt actin pre-mRNA

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Expression

   Bacterial

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBW400hrpL-gfp

   Plasmid

   #61437

   Purpose

   pBW400hrpL-gfp

   Depositor

   Martin Buck, Baojun Wang

   Article

   Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

   Insert

   hrpL-rbs30-gfp-ter
   

   Expression

   Bacterial

   Promoter

   HrpL

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX2TK-Num1-PH

   Plasmid

   #71369

   Purpose

   bacterial expression of PH domain for phosphoinositide binding assay

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Num1-PH
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa2563-2692

   Promoter

   tac

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pTD103luxI_sfGFP -R

    Plasmid

    #48887

    Purpose

    Positive feedback plasmid from oscillator without luxR

    Depositor

    Jeff Hasty

    Article

    Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

    Insert

    sfGFP and luxI
    

    Use

    Synthetic Biology

    Tags

    ssrA tag (AANDENYALAA)

    Expression

    Bacterial

    Promoter

    pLux

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-DUET-GST-eIF4G1-His6, eIF4E

    Plasmid

    #37232

    Depositor

    Jon Lorsch

    Article

    Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

    Inserts

    TIF4631 (TIF4631 Budding Yeast)
    CDC33 (CDC33 Budding Yeast)
    

    Tags

    GST and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

12. HC89 pComb3xss anti-Homer1 nanobody

    Plasmid

    #134719

    Purpose

    Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-Homer1 (Mouse) recombinant llama nanobody HC89 (Homer1 L. glama (llama))
    

    Tags

    6xHis-HA

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a His6Flag2AUF1(p37)

    Plasmid

    #135939

    Purpose

    Expresses AUF1p37 in E. coli

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
    

    Tags

    Flag2 and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHL 1903

    Plasmid

    #53020

    Purpose

    AmpR + fimS::gfpAAV::Asp terminator + CamR + ColE1

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    AAV, Asp terminator, and fimS

    Expression

    Bacterial

    Promoter

    fimS

    Available Since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pHis-zfCSF3a

    Plasmid

    #65612

    Purpose

    express soluble His-tagged zebrafish CSF3a/Gcsfa in E. coli

    Depositor

    Petr Bartunek

    Article

    Stachura et al Blood. 2011 Aug 4;118(5):1274-82. doi: 1

    Insert

    Csf3a (csf3a Zebrafish)
    

    Tags

    (His)6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pZE27GFP5

    Plasmid

    #75458

    Purpose

    Plasmid for constitutive expression of gfp-pdt5

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    gfp-pdt#5
    

    Expression

    Bacterial

    Promoter

    placIq promoter

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid

    #107582

    Purpose

    B. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

    Depositor

    Paul Freemont

    Article

    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

    Insert

    GFP+
    

    Use

    Synthetic Biology; E. coli and bacillus shuttle v…

    Tags

    B. megaterium DSM319 xylA leader sequence (MTSSKI…

    Expression

    Bacterial

    Mutation

    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

    Promoter

    fba promoter Bacillus megaterium DSM319

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHH0103_PIKFYVE_DEP

    Plasmid

    #109789

    Purpose

    Protein expression and purification of PIKFYVE_DEP

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PIKFYVE_DEP (PIKFYVE Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SRpHi1

    Plasmid

    #102541

    Purpose

    Bacterial expression of EYFP fused to the cell penetrating peptide TAT with a GST tag for purification

    Depositor

    Stefan Hell

    Article

    Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

    Insert

    Tat-EYFP
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pH6HTC_BTF3-Halo

    Plasmid

    #175324

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    BTF3 (BTF3 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge