31,828 results

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pScaf-10080.1

Plasmid

#111410

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 10080 bases in length

Depositor

Shawn Douglas

Article

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-ACKN

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pOPO717

Plasmid

#195302

Purpose

pBAD322-MycaIDCas-lacZsp5, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is lacZ spacer 5.

Depositor

Joseph Peters

Article

Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

Insert

McCAST Cascade and lacZ spacer 5 in native array

Expression

Bacterial

Promoter

Arabinose inducible arapBAD

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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GFP S2TAG

Plasmid

#68296

Purpose

S2TAG GFP reporter

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

GFP S2TAG

Tags

His

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pShoCAST-sgRNA_entry (BO1)

Plasmid

#181786

Purpose

Expresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k

Expression

Bacterial

Promoter

Lac and J23119

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pShoHELIX-sgRNA_entry (BO3)

Plasmid

#181784

Purpose

Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k

Expression

Bacterial

Mutation

nAniI = K227M, F80K, L232K

Promoter

Lac and J23119

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe-PI(WT)-Src(YF)-mCherry

Plasmid

#87357

Purpose

PI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutation

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Src isoform 2 (Src Mouse)

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Y535F

Promoter

CMV

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLacI241-Wasabi

Plasmid

#86102

Purpose

mWasabi reporter gene gated by temperature-sensitive LacI A241T

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

LacIts
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

A241T

Promoter

T7A1O4/O3 and pLacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBEST-PLtetO-1-UTR1-TetR-deGFP-T500

Plasmid

#45774

Depositor

Richard Murray

Insert

tetR-deGFP

Use

Synthetic Biology

Tags

Fusion protein of TetR and deGFP, with glycine-se…

Expression

Bacterial

Promoter

PLtetO-1

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only

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pET-3c-rhVTN-C

Plasmid

#30224

Depositor

James Thomson

Article

Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

Insert

rhVitronectin with C terminus cleavage (VTN Human)

Expression

Bacterial

Mutation

deleted amino acids: 399-478

Available Since

May 24, 2011

Availability

Academic Institutions and Nonprofits only

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Reb_207 aka PAS630

Plasmid

#71235

Purpose

Reb_206 with RebA(S88P)

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

mNeonGreen to second copy RebB, soluble mCherry a…

Expression

Bacterial

Mutation

RebA(S88P)

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pRS413-GAL1-luc*(-SKL)

Plasmid

#40234

Depositor

Michael Benton

Insert

luc*(-SKL)

Expression

Bacterial

Promoter

GAL1

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pET-45b-RUNX2(WT)-IDR-mCherry

Plasmid

#145286

Purpose

Bacterial Expression of 6xHis RUNX2(WT) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2(WT)-IDR (RUNX2 Human)

Expression

Bacterial

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pTriEx-mVenus-Zdk1-Rac1 Q61L

Plasmid

#81137

Purpose

mammalian expression of Zdk1 tagged Rac1 Q61L

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
Rac1 (RHOA Human)

Tags

mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Q61L

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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R989-X09-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D

Plasmid

#159541

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G12D

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCoofy5

Plasmid

#43989

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6-Sumo1

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX ShcD-SH2

Plasmid

#46488

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

ShcD (SHC4 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 512-623

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pIT5_KT

Plasmid

#173773

Purpose

Bacterial genomic integration vector

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pIT5_KH

Plasmid

#173772

Purpose

Bacterial genomic integration vector

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-His6-Ubc9-K14R

Plasmid

#185759

Purpose

Bacterial expression of His6-Ubc9-K14R

Depositor

Jeffrey Bode

Article

Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

K14R

Promoter

T7

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pTE104

Plasmid

#59399

Purpose

MethyloBrick expression vector, coxB promoter; TcR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PcoxB

Available Since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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