Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
Bacterial Expression Clear filter

Applied Filters

Clear All Filters
Bacterial Expression Clear filter

33,532 results

Showing: 9721 - 9740 of 33532 results

  3. pJL33

    Plasmid
    #227430
    Purpose
    Kanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)
    Depositor
    Pierre Santucci
    Publication
    Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:
    Insert
    mWasabi
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    227430-plasmid-map-sequence-id-455969

    Enlarge

  4. pJL34

    Plasmid
    #227431
    Purpose
    Kanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)
    Depositor
    Pierre Santucci
    Publication
    Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:
    Insert
    tdTomato
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    227431-plasmid-map-sequence-id-455970

    Enlarge

  5. pZP06B

    Plasmid
    #232321
    Purpose
    Make sacB a counterselection marker to replace galK
    Depositor
    Chenggang Wu
    Insert
    PrpsJ-sacB
    Expression
    Bacterial
    Available since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    232321-plasmid-map-sequence-id-462085

    Enlarge

  8. pET-Impact-T4gp43(exo-)

    Plasmid
    #228202
    Purpose
    Expression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219A
    Depositor
    Stephen Benkovic
    Insert
    43
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Mutated aspartate 219 to alanine (D219A), elimina…
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228202-plasmid-map-sequence-id-453943

    Enlarge

  11. pCWU50

    Plasmid
    #232318
    Purpose
    A conditional plasmid system where RepA, required for pCWU6-based plasmid replication, is regulated by an inducible fdx promoter and theophylline-responsive riboswitch
    Depositor
    Chenggang Wu
    Insert
    Pfdx-E
    Expression
    Bacterial
    Promoter
    fdx
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232318-plasmid-map-sequence-id-462281

    Enlarge

  13. ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain

    Plasmid
    #228663
    Purpose
    Bacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)
    Depositor
    ASAP Discovery, Frank von Delft
    Insert
    Zika virus portion of NS2B protease coexpressed with NS3 protease
    Tags
    His-GST-TEV
    Expression
    Bacterial
    Available since
    Feb. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    228663-plasmid-map-sequence-id-461604

    Enlarge

  14. pGLO-Yellow-AZA

    Plasmid
    #220783
    Purpose
    Expresses a yellow chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)
    Depositor
    Angela Leu
    Insert
    yellow chromoprotein
    Tags
    6xHis
    Expression
    Bacterial
    Available since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    220783-plasmid-map-sequence-id-444833

    Enlarge

  15. pEND-350_Cacnes_ori

    Plasmid
    #225605
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)
    Depositor
    Marc Güell
    Publication
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225605-plasmid-map-sequence-id-451372

    Enlarge

  18. pSW031

    Plasmid
    #231144
    Purpose
    Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.
    Depositor
    Philip Cole
    Publication
    Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021
    Insert
    S. aureus Sortase A mutant
    Tags
    Nostoc punctiform split intein C-terminal fragmen…
    Expression
    Bacterial
    Mutation
    P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…
    Promoter
    T7
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231144-plasmid-map-sequence-id-460063

    Enlarge

  20. pET28a_SYK

    Plasmid
    #224240
    Purpose
    Expression of human SYK Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    syk (SYK Human)
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 promotor
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    224240-plasmid-map-sequence-id-459259

    Enlarge

Showing: 9721 - 9740 of 33532 results