30,535 results
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Plasmid#169020PurposeExpresses His-p97 in bacteriaDepositorInsertp97
UseTagsHisExpressionBacterialMutationPromoterT7Available SinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-hALIX
Plasmid#21504DepositorAvailable SinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
HTATIP (2OU2)
Plasmid#33338PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHTATIP (KAT5 Human)
UseTagsHexahistidine tag with Thrombin cleavageExpressionBacterialMutationPromoterT7Available SinceJan. 11, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSP64T (DM#111)
Plasmid#15030DepositorInsertbeta-globin 5' and 3' UTR
UseTagsExpressionBacterialMutationPromoterAvailable SinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNeae2
Plasmid#168300PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
UseTags6xHis, E-tag, and myc-tagExpressionBacterialMutationPromoterLacI-PlacAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-CL7-2NLS-SpyCas9(dS)-A22p3star
Plasmid#222970PurposeBacterial expression of A22p-fused SpyCas9 constructDepositorInsertHis-CL7-2NLS-SpyCas9(dS)-A22p3
UseCRISPRTagsCL7 and HisExpressionBacterialMutationPromotertacAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUS116
Plasmid#84578PurposeShuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceJan. 17, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYSD061
Plasmid#212915PurposeEncodes the S. cerevisiae SUC2 (Invertase) protein (minus signal peptide) as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET283xFlagPP7CPHis
Plasmid#28174DepositorInsertPP7 coat protein optimized
UseTags6xhistidine and triple FLAG peptideExpressionBacterialMutationC68A,C73APromoterAvailable SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDF-5xT7-TtCsm
Plasmid#128572PurposeFor expression of T. thermophilus Csm in E. coli (Csm1-5)DepositorInsertsCsm1
Csm2, Csm3, Csm4, Csm5
UseTagsHRV 3C protease cleavage site and 10xHis tag on C…ExpressionBacterialMutationCodon optimized for expression in E. coli and Cod…PromoterT7 promoter, lac operatorAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
LCC-ICCG_pET21b
Plasmid#215408PurposeExpression construct for LCC-ICCG gene, codon optimized for expression in E. coliDepositorInsertLCC-ICCG
UseTagsExpressionBacterialMutationF243I/D238C/S283C/Y127GPromoterAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
1GFP/RNase H1 WT
Plasmid#174447PurposeExpress GFP-tagged RNase H1 WT in E. coli.DepositorInsertRNase H1 (RNASEH1 Human)
UseTagsHis-tag, GFPExpressionBacterialMutationPromoterT7 promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pG1K
Plasmid#71741PurposeModular shuttle vector for Geobacillus and E. coliDepositorTypeEmpty backboneUseSynthetic Biology; Shuttle vectorTagsExpressionBacterialMutationPromoterAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
hUbc13
Plasmid#51131Purposehuman ubc13, E2 for synthesis k63 linked ubiquitin chainDepositorAvailable SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINJ
Plasmid#26045DepositorInsertHisGST3C-lacZ
UseTagsMAHHHHHH_GST_LEVLFQGTExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable SinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
plRL19
Plasmid#163634PurposeL5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).DepositorInsertL5 Integrase
UseTagsExpressionBacterialMutationPromoterMycobacterial Optimized Promoter (MOP) and Tet-OnAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Hyg)
Plasmid#110084PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCWU6-xylR-E-luciferase
Plasmid#190721Purposefine tune expression of luciferase under the control of xylose- and theophylline- inducible systemDepositorInsertxylR-E-luciferase
UseLuciferaseTagsExpressionBacterialMutationPromoterAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM1s3AsG
Plasmid#137921PurposeE. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expressionDepositorInsertSuperfolder GFP
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETDuet S2
Plasmid#171921PurposeExpress RNA-based (p)ppGpp sensor and a NirFPDepositorInsertsRNA-based (p)ppGpp sensor
NirFP
UseTagsExpressionBacterialMutationPromoterT7Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only